Construct: ORF ccsbBroad304_15276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006830.2_s304c1, BRDN0000459522
- Derived from:
- ccsbBroadEn_15276
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCLK2 (166614)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15276
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_017007832.2 | 96.4% | 95.8% | (many diffs) |
| 2 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_005262786.3 | 90.7% | 90% | (many diffs) |
| 3 | human | 166614 | DCLK2 | doublecortin like kinase 2 | NM_001040260.4 | 90.6% | 89.9% | (many diffs) |
| 4 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_005262785.3 | 88.7% | 88.1% | (many diffs) |
| 5 | human | 166614 | DCLK2 | doublecortin like kinase 2 | NM_001040261.5 | 88.6% | 88% | (many diffs) |
| 6 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_024453914.1 | 85.1% | 85% | 1776_1776delGins310 |
| 7 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XR_244621.3 | 82.1% | (many diffs) | |
| 8 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_024453915.1 | 79.3% | 79.3% | 959_1009del;1106_1108delAGC;1752_1752delGins388 |
| 9 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_024453913.1 | 78.9% | 78.3% | (many diffs) |
| 10 | human | 166614 | DCLK2 | doublecortin like kinase 2 | XM_017007833.2 | 77.2% | 76.6% | (many diffs) |
| 11 | human | 166614 | DCLK2 | doublecortin like kinase 2 | NR_036614.2 | 50.1% | 1_583del;2669_4155del | |
| 12 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502071.2 | 89.9% | 96.5% | (many diffs) |
| 13 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_017319718.1 | 87.9% | 94.3% | (many diffs) |
| 14 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502069.2 | 87.8% | 94.2% | (many diffs) |
| 15 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195499.1 | 87% | 92.7% | (many diffs) |
| 16 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195498.1 | 86.8% | 92.6% | (many diffs) |
| 17 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502074.2 | 84.3% | 90.5% | (many diffs) |
| 18 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502068.2 | 84.1% | 88.2% | (many diffs) |
| 19 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502072.2 | 84.1% | 90.1% | (many diffs) |
| 20 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502073.2 | 84.1% | 90.1% | (many diffs) |
| 21 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502075.2 | 83.8% | 90% | (many diffs) |
| 22 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195497.1 | 82.5% | 87.9% | (many diffs) |
| 23 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_027539.5 | 82.4% | 87.8% | (many diffs) |
| 24 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195496.1 | 80.8% | 86.1% | (many diffs) |
| 25 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502065.2 | 80.7% | 86% | (many diffs) |
| 26 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502070.2 | 80.7% | 84.7% | (many diffs) |
| 27 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502076.2 | 80.6% | 86.5% | (many diffs) |
| 28 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502077.2 | 80.6% | 86.5% | (many diffs) |
| 29 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502078.2 | 80.2% | 86.3% | (many diffs) |
| 30 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502067.2 | 79.1% | 84.4% | (many diffs) |
| 31 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_011240226.1 | 78.1% | 78.9% | (many diffs) |
| 32 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_006502066.2 | 77.5% | 82.7% | (many diffs) |
| 33 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_011240227.1 | 76.7% | 79.3% | (many diffs) |
| 34 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XM_017319719.1 | 76.5% | 77.9% | (many diffs) |
| 35 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | NM_001195500.1 | 76.1% | 81.7% | (many diffs) |
| 36 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XR_867231.2 | 58% | (many diffs) | |
| 37 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XR_867230.2 | 51.5% | (many diffs) | |
| 38 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XR_001783745.1 | 45% | (many diffs) | |
| 39 | mouse | 70762 | Dclk2 | doublecortin-like kinase 2 | XR_867229.1 | 28.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2151
- ORF length:
- 2085
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcaccagg agtatcgagc tggagcactt tgaggaacgg gacaaaaggc 121 cgcggccggg gtcgcggaga ggggccccca gctcctccgg gggcagcagc agctcgggcc 181 ccaaggggaa cgggctcatc cccagtccgg cgcacagtgc ccactgcagc ttctaccgca 241 cgcggaccct gcaggccctc agctcggaga agaaggccaa gaaggcgcgc ttctaccgga 301 acggggaccg ctacttcaag ggcctggtgt ttgccatctc cagcgaccgc ttccggtcct 361 tcgatgcgct cctcatagag ctcacccgct ccctgtcgga caacgtgaac ctgccccagg 421 gtgtccgcac tatctacacc atcgacggca gccggaaggt caccagcctg gacgagctgc 481 tggaaggtga gagttacgtg tgtgcatcca atgaaccatt tcgtaaagtc gattacacca 541 aaaatattaa tccaaactgg tctgtgaaca tcaagggtgg gacatcccga gcgctggctg 601 ctgcctcctc tgtgaaaagt gaagtaaaag aaagtaaaga tttcatcaaa cccaagttag 661 tgactgtgat tcgaagtgga gtgaagccta gaaaagccgt gcggatcctt ctgaataaaa 721 agactgctca ttcctttgaa caagtcttaa cagatatcac cgaagccatt aaactagact 781 caggagtcgt caagaggctc tgcaccctgg atggaaagca ggttacttgt ctgcaagact 841 tttttggtga tgacgatgtt tttattgcat gtggaccaga aaaatttcgt tatgcccaag 901 atgactttgt cctggatcat agtgaatgtc gtgtcctgaa gtcatcttat tctcgatcct 961 cagctgttaa gtattctgga tccaaaagcc ctgggccctc tcgacgcagc aaatcaccag 1021 cttcagttaa tggaactccc agcagccaac tttctactcc taaatctacg aaatcctcca 1081 gttcctctcc aactagtcca ggaagtttca gaggattaaa gatttctgct catggcagat 1141 cttcttccaa tgtaaacggt ggacctgagc ttgaccgttg cataagtcct gaaggtgtga 1201 atggaaacag atgctctgaa tcatcaactc ttcttgagaa atacaaaatt ggaaaggtca 1261 ttggtgatgg caattttgca gtagtcaaag agtgtataga caggtccact ggaaaggagt 1321 ttgccctaaa gattatagac aaagccaaat gttgtggaaa ggaacacctg attgagaatg 1381 aagtgtcaat actgcgccga gtgaaacatc ccaatatcat tatgctggtc gaggagatgg 1441 aaacagcaac tgagctcttt ctggtgatgg aattggtcaa aggtggagat ctctttgatg 1501 caattacttc gtcgaccaag tacactgaga gagatggcag tgccatggtg tacaacttag 1561 ccaatgccct caggtatctc catggcctca gcatcgtgca cagagacatc aaaccagaga 1621 atctcttggt gtgtgaatat cctgatggaa ccaagtcttt gaaactggga gactttgggc 1681 ttgcgactgt ggtagaaggc cctttataca cagtctgtgg cacacccact tatgtggctc 1741 cagaaatcat tgctgaaact ggctatggcc tgaaggtgga catttgggca gctggtgtga 1801 tcacatacat acttctctgt ggattcccac cattccgaag tgagaacaat ctccaggaag 1861 atctcttcga ccagatcttg gctgggaagc tggagtttcc ggccccctac tgggataaca 1921 tcacggactc tgccaaggaa ttaatcagtc aaatgcttca ggtaaatgtt gaagctcggt 1981 gtaccgcggg acaaatcctg agtcacccct gggtgtcaga tgatgcctcc caggagaata 2041 acatgcaagc tgaggtgaca ggtaaactaa aacagcactt taataatgcg ctccccaaac 2101 agaacagcac taccaccggg gtctccgtca tcatgtttga tttgacagtt tgcccaactt 2161 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2221 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta