Construct: ORF ccsbBroad304_15311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009579.1_s304c1, BRDN0000408445
- Derived from:
- ccsbBroadEn_15311
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCE2A (353139)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15311
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 353139 | LCE2A | late cornified envelope 2A | NM_178428.4 | 98.7% | 97.1% | 173G>A;295T>G;298_299delCAinsTC |
| 2 | human | 353141 | LCE2D | late cornified envelope 2D | NM_178430.4 | 87.5% | 85.4% | (many diffs) |
| 3 | human | 26239 | LCE2B | late cornified envelope 2B | NM_014357.5 | 86.6% | 84.5% | (many diffs) |
| 4 | human | 353140 | LCE2C | late cornified envelope 2C | NM_178429.3 | 86.6% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 387
- ORF length:
- 318
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcctgccag caaaaccagc agcagtgcca gccccctccc aagtgccccc 121 caaaatgccc acccaagtgt cctccaaagt gccgacctca gtgcccagcc ccatgcccac 181 ctccagtctc ttcctgctgt ggtcccagct ctgggggctg ctgcggctcc agctctgggg 241 actgctgcag ctctgggggt ggcggctgct gcctgagcca ccacaggccc cgtctcttcc 301 accggcaccg gcaccagagc cccgattgtt gtgagtgtga accttctggg ggctctggct 361 gcggctccag ctctggggac tgctgcttgc caactttctt gtacaaagtg gttggtaagc 421 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 481 ggcgcgttaa gtcgacaatc aacctctgga tta