Construct: ORF ccsbBroad304_15312
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006421.1_s304c1, BRDN0000401377
- Derived from:
- ccsbBroadEn_15312
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCE2D (353141)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15312
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 353141 | LCE2D | late cornified envelope 2D | NM_178430.4 | 100% | 100% | |
| 2 | human | 353140 | LCE2C | late cornified envelope 2C | NM_178429.3 | 97.8% | 96.3% | (many diffs) |
| 3 | human | 26239 | LCE2B | late cornified envelope 2B | NM_014357.5 | 96.3% | 94.5% | (many diffs) |
| 4 | human | 353139 | LCE2A | late cornified envelope 2A | NM_178428.4 | 88.7% | 88.1% | (many diffs) |
| 5 | human | 254910 | LCE5A | late cornified envelope 5A | NM_178438.5 | 70.1% | 68.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 399
- ORF length:
- 330
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcttgccag caaaaccagc agcagtgcca gccccctccc aaatgtcctc 121 ccaagtgtac cccaaaatgt ccacctaagt gtccccccaa atgcccacca cagtgcccag 181 ctccatgttc ccctgcagtc tcttcctgct gtggtcccag ctctgggagc tgctgtggtc 241 ccagctctgg gggctgctgc agctctgggg gtggtggctg ctgcctgagc caccacaggc 301 cccgtctctt ccaccggcgc cggcaccaga gccccgattg ctgtgagagt gaaccttctg 361 gggcctctgg ctgctgccac agctctgggg gctgctgctt gccaactttc ttgtacaaag 421 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 481 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta