Construct: ORF ccsbBroad304_15328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009425.1_s304c1, BRDN0000460417
- Derived from:
- ccsbBroadEn_15328
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PMS2CL (441194)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15328
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322011.2 | 34.5% | 34.4% | 1_1080del;1320T>C;1391A>G |
| 2 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322012.2 | 34.5% | 34.4% | 1_1080del;1320T>C;1391A>G |
| 3 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | XM_006715744.4 | 34.5% | 34.4% | 1_1080del;1320T>C;1391A>G |
| 4 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | XM_017012342.2 | 34.5% | 34.4% | 1_1080del;1320T>C;1391A>G |
| 5 | human | 441194 | PMS2CL | PMS2 C-terminal like pseudo... | NR_002217.1 | 32.9% | 1_953del;1527_1738del | |
| 6 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322013.2 | 28.3% | 28.3% | 1_1440del;1680T>C;1751A>G |
| 7 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322010.2 | 28.1% | 28.1% | 1_1452del;1692T>C;1763A>G |
| 8 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | XM_024446800.1 | 28.1% | 28.1% | 1_1452del;1692T>C;1763A>G |
| 9 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322003.2 | 26.1% | 26.1% | 1_1608del;1848T>C;1919A>G |
| 10 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322004.2 | 26.1% | 26.1% | 1_1608del;1848T>C;1919A>G |
| 11 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322005.2 | 26.1% | 26.1% | 1_1608del;1848T>C;1919A>G |
| 12 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322009.2 | 25.7% | 25.7% | (many diffs) |
| 13 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322007.1 | 25.1% | 25.1% | 1_1695del;1935T>C;2006A>G |
| 14 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322008.2 | 25.1% | 25.1% | 1_1695del;1935T>C;2006A>G |
| 15 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322015.2 | 25% | 25% | 1_1704del;1944T>C;2015A>G |
| 16 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322006.2 | 23.4% | 23.4% | 1_1857del;2097T>C;2168A>G |
| 17 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_000535.7 | 22% | 22% | 1_2013del;2253T>C;2324A>G |
| 18 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NM_001322014.2 | 21.8% | 21.7% | (many diffs) |
| 19 | human | 5395 | PMS2 | PMS1 homolog 2, mismatch re... | NR_136154.1 | 10.2% | (many diffs) | |
| 20 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XM_006504812.2 | 24.9% | 26% | (many diffs) |
| 21 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XM_017320710.1 | 24.9% | 26% | (many diffs) |
| 22 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | NM_008886.2 | 19.5% | 20.4% | (many diffs) |
| 23 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XR_001784636.1 | 16.8% | (many diffs) | |
| 24 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XR_001784637.1 | 16.7% | (many diffs) | |
| 25 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XR_377191.3 | 14% | (many diffs) | |
| 26 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XR_001784638.1 | 12.1% | (many diffs) | |
| 27 | mouse | 18861 | Pms2 | postmeiotic segregation inc... | XR_001784639.1 | 12% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 642
- ORF length:
- 573
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttgcagaa atggaaatca ttggtcagtt taacctggga tttataataa 121 ccaaactgaa tgaggatatc ttcatagtgg accagcatgc cacggacgag aagtataact 181 tcgagatgct gcagcagcac accgtgctcc aggggcagag gctcatagca cctcagactc 241 tcaacttaac tgctgttaat gaagctgttc tgatagaaaa tctggaaata tttagaaaga 301 atggcttcga ttttgttatc gatgaaaatg ctccagtcac tgaaagggct aaactgattt 361 ccttgccaac tagtaaaagc tggaccttcg gaccccagga cgtcgatgaa ctgatcttca 421 tgctgagcga cagccctggg gtcatgtgcc ggccttcccg agtcaagcag atgtttgcct 481 ccagagcctg ccggaagtcg gtgatgattg ggactgctct taacacaagc gagatgaaga 541 aactgatcac ccacatgggg gagatggacc acccctggaa ctgtccccat ggaaggccaa 601 ccatgagaca catcgccaac ctgggtgtca tttctcagaa cttgccaact ttcttgtaca 661 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 721 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta