Transcript: Human NM_001322012.2

Homo sapiens PMS1 homolog 2, mismatch repair system component (PMS2), transcript variant 11, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
PMS2 (5395)
Length:
5039
CDS:
910..2565

Additional Resources:

NCBI RefSeq record:
NM_001322012.2
NBCI Gene record:
PMS2 (5395)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001322012.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000420228 TGATTCAGAATGCGTTGATAT pLKO_005 957 CDS 100% 13.200 18.480 N PMS2 n/a
2 TRCN0000078549 CGTGTGTGAAGAGTACGGTTT pLKO.1 726 5UTR 100% 4.050 5.670 N PMS2 n/a
3 TRCN0000078551 GACCTCTTTGATAGGAATGTT pLKO.1 1044 CDS 100% 5.625 3.938 N PMS2 n/a
4 TRCN0000425805 GGACTATGGAGTGGATCTTAT pLKO_005 202 5UTR 100% 13.200 7.920 N PMS2 n/a
5 TRCN0000423170 CACCTCAGACTCTCAACTTAA pLKO_005 2150 CDS 100% 13.200 6.600 Y PMS2 n/a
6 TRCN0000256790 CTGTGCGCCATAAGGAATTTC pLKO_005 525 5UTR 100% 13.200 6.600 Y PMS2P9 n/a
7 TRCN0000078552 CCAGTCACTGAAAGGGCTAAA pLKO.1 2254 CDS 100% 10.800 5.400 Y PMS2 n/a
8 TRCN0000078548 CCAGGAAGATACCGGATGTAA pLKO.1 1638 CDS 100% 5.625 2.813 Y PMS2 n/a
9 TRCN0000107284 CGGAAGTCAGTCCATCAGATT pLKO.1 83 5UTR 100% 4.950 2.475 Y PMS2P3 n/a
10 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 4100 3UTR 100% 4.950 2.475 Y n/a
11 TRCN0000078550 CGAGAAGTATAACTTCGAGAT pLKO.1 2088 CDS 100% 4.050 2.025 Y PMS2 n/a
12 TRCN0000118012 CCATAAGGAATTTCAAAGGAA pLKO.1 532 5UTR 100% 3.000 1.500 Y PMS2P5 n/a
13 TRCN0000240631 AGTCACTGAAAGGGCTAAATT pLKO_005 2256 CDS 100% 15.000 7.500 Y Pms2 n/a
14 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4173 3UTR 100% 5.625 2.813 Y KLHL30 n/a
15 TRCN0000130146 CAGGTTCAAGTGATTCTCCTA pLKO.1 4009 3UTR 100% 2.640 1.320 Y DICER1-AS1 n/a
16 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4173 3UTR 100% 5.625 2.813 Y EID2B n/a
17 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 3007 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001322012.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14772 pDONR223 92.2% 63.8% 63.6% None 0_1ins933;688A>G;933G>A n/a
2 ccsbBroad304_14772 pLX_304 0% 63.8% 63.6% V5 0_1ins933;688A>G;933G>A n/a
3 TRCN0000480058 GCTATCTCATTGCACGAGGCGAAG pLX_317 12.6% 63.8% 63.6% V5 0_1ins933;688A>G;933G>A n/a
4 ccsbBroadEn_15328 pDONR223 73.3% 34.5% 34.4% None 1_1080del;1320T>C;1391A>G n/a
5 ccsbBroad304_15328 pLX_304 0% 34.5% 34.4% V5 1_1080del;1320T>C;1391A>G n/a
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