Transcript: Human NM_001322006.2

Homo sapiens PMS1 homolog 2, mismatch repair system component (PMS2), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
PMS2 (5395)
Length:
4937
CDS:
31..2463

Additional Resources:

NCBI RefSeq record:
NM_001322006.2
NBCI Gene record:
PMS2 (5395)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001322006.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000078549 CGTGTGTGAAGAGTACGGTTT pLKO.1 780 CDS 100% 4.050 5.670 N PMS2 n/a
2 TRCN0000425805 GGACTATGGAGTGGATCTTAT pLKO_005 207 CDS 100% 13.200 7.920 N PMS2 n/a
3 TRCN0000423170 CACCTCAGACTCTCAACTTAA pLKO_005 2048 CDS 100% 13.200 6.600 Y PMS2 n/a
4 TRCN0000256790 CTGTGCGCCATAAGGAATTTC pLKO_005 530 CDS 100% 13.200 6.600 Y PMS2P9 n/a
5 TRCN0000078552 CCAGTCACTGAAAGGGCTAAA pLKO.1 2152 CDS 100% 10.800 5.400 Y PMS2 n/a
6 TRCN0000078548 CCAGGAAGATACCGGATGTAA pLKO.1 1536 CDS 100% 5.625 2.813 Y PMS2 n/a
7 TRCN0000107284 CGGAAGTCAGTCCATCAGATT pLKO.1 88 CDS 100% 4.950 2.475 Y PMS2P3 n/a
8 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 3998 3UTR 100% 4.950 2.475 Y n/a
9 TRCN0000078550 CGAGAAGTATAACTTCGAGAT pLKO.1 1986 CDS 100% 4.050 2.025 Y PMS2 n/a
10 TRCN0000118012 CCATAAGGAATTTCAAAGGAA pLKO.1 537 CDS 100% 3.000 1.500 Y PMS2P5 n/a
11 TRCN0000240631 AGTCACTGAAAGGGCTAAATT pLKO_005 2154 CDS 100% 15.000 7.500 Y Pms2 n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4071 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000130146 CAGGTTCAAGTGATTCTCCTA pLKO.1 3907 3UTR 100% 2.640 1.320 Y DICER1-AS1 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4071 3UTR 100% 5.625 2.813 Y EID2B n/a
15 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2905 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001322006.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14772 pDONR223 92.2% 93.8% 93.7% None (many diffs) n/a
2 ccsbBroad304_14772 pLX_304 0% 93.8% 93.7% V5 (many diffs) n/a
3 TRCN0000480058 GCTATCTCATTGCACGAGGCGAAG pLX_317 12.6% 93.8% 93.7% V5 (many diffs) n/a
4 ccsbBroadEn_15328 pDONR223 73.3% 23.4% 23.4% None 1_1857del;2097T>C;2168A>G n/a
5 ccsbBroad304_15328 pLX_304 0% 23.4% 23.4% V5 1_1857del;2097T>C;2168A>G n/a
6 ccsbBroadEn_11040 pDONR223 100% 10.2% 8.7% None (many diffs) n/a
7 ccsbBroad304_11040 pLX_304 0% 10.2% 8.7% V5 (many diffs) n/a
8 TRCN0000477902 TCACAGCCGGGTCATAGTAAATTT pLX_317 76.4% 10.2% 8.7% V5 (many diffs) n/a
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