Construct: ORF ccsbBroad304_15367
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006579.1_s304c1, BRDN0000401487
- Derived from:
- ccsbBroadEn_15367
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BARD1 (580)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15367
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | NM_000465.4 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | NM_001282543.2 | 97.3% | 97.1% | (many diffs) |
| 3 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | XM_017004613.1 | 95.7% | 95.4% | (many diffs) |
| 4 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | XR_002959322.1 | 81.9% | (many diffs) | |
| 5 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | XM_017004614.1 | 78% | 77.9% | (many diffs) |
| 6 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | NM_001282545.2 | 41.8% | 41.8% | 70C>T;84G>T;215_216ins1353 |
| 7 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | NR_104212.2 | 39.7% | (many diffs) | |
| 8 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | NR_104215.2 | 38.7% | (many diffs) | |
| 9 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | NM_001282548.2 | 36% | 39.3% | (many diffs) |
| 10 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | NM_001282549.2 | 33.8% | 33.7% | 70C>T;84G>T;364_365ins1539 |
| 11 | human | 580 | BARD1 | BRCA1 associated RING domain 1 | NR_104216.2 | 25.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2400
- ORF length:
- 2331
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gccggataat cggcagccga ggaaccggca gccgaggatc cgctccggga 121 acgagcctcg ttccgcgtcc gccatggaac ctgatggtcg cggtgcctgg gcccacagtc 181 gcgccgcgct cgaccgcctg gagaagctgc tgcgctgctc gcgttgtact aacattctga 241 gagagcctgt gtgtttagga ggatgtgagc acatcttctg tagtaattgt gtaagtgact 301 gcattggaac tggatgtcca gtgtgttaca ccccggcctg gatacaagac ttgaagataa 361 atagacaact ggacagcatg attcaacttt gtagtaagct tcgaaatttg ctacatgaca 421 atgagctgtc agatttgaaa gaagataaac ctaggaaaag tttgtttaat gatgcaggaa 481 acaagaagaa ttcaattaaa atgtggttta gccctcgaag taagaaagtc agatatgttg 541 tgagtaaagc ttcagtgcaa acccagcctg caataaaaaa agatgcaagt gctcagcaag 601 actcatatga atttgtttcc ccaagtcctc ctgcagatgt ttctgagagg gctaaaaagg 661 cttctgcaag atctggaaaa aagcaaaaaa agaaaacttt agctgaaatc aaccaaaaat 721 ggaatttaga ggcagaaaaa gaagatggtg aatttgactc caaagaggaa tctaagcaaa 781 agctggtatc cttctgtagc caaccatctg ttatctccag tcctcagata aatggtgaaa 841 tagacttact agcaagtggc tccttgacag aatctgaatg ttttggaagt ttaactgaag 901 tctctttacc attggctgag caaatagagt ctccagacac taagagcagg aatgaagtag 961 tgactcctga gaaggtctgc aaaaattatc ttacatctaa gaaatctttg ccattagaaa 1021 ataatggaaa acgtggccat cacaatagac tttccagtcc catttctaag agatgtagaa 1081 ccagcattct gagcaccagt ggagattttg ttaagcaaac ggtgccctca gaaaatatac 1141 cattgcctga atgttcttca ccaccttcat gcaaacgtaa agttggtggt acatcaggga 1201 gcaaaaacag taacatgtcc gatgaattca ttagtctttc accaggtaca ccaccttcta 1261 cattaagtag ttcaagttac aggcgagtga tgtctagtcc ctcagcaatg aagctgttgc 1321 ccaatatggc tgtgaaaaga aatcatagag gagagacttt gctccatatt gcttctatta 1381 agggcgacat accttctgtt gaataccttt tacaaaatgg aagtgatcca aatgttaaag 1441 accatgctgg atggacacca ttgcatgaag cttgcaatca tgggcacctg aaggtagtgg 1501 aattattgct ccagcataag gcattggtga acaccaccgg gtatcaaaat gactcaccac 1561 ttcacgatgc agccaagaat gggcacatgg atatagtcaa gctgttactt tcctatggag 1621 cctccagaaa tgctgttaat atatttggtc tgcggcctgt cgattataca gatgatgaaa 1681 gtatgaaatc gctattgctg ctaccagaga agaatgaatc atcctcagct agccactgct 1741 cagtaatgaa cactgggcag cgtagggatg gacctcttgt acttataggc agtgggctgt 1801 cttcagaaca acagaaaatg ctcagtgagc ttgcagtaat tcttaaggct aaaaaatata 1861 ctgagtttga cagtacagta actcatgttg ttgttcctgg tgatgcagtt caaagtacct 1921 tgaagtgtat gcttgggatt ctcaatggat gctggattct aaaatttgaa tgggtaaaag 1981 catgtctacg aagaaaagta tgtgaacagg aagaaaagta tgaaattcct gaaggtccac 2041 gcagaagcag gctcaacaga gaacagctgt tgccaaagct gtttgatgga tgctacttct 2101 atttgtgggg aaccttcaaa caccatccaa aggacaacct tattaagctc gtcactgcag 2161 gtgggggcca gatcctcagt agaaagccca agccagacag tgacgtgact cagaccatca 2221 atacagtcgc ataccatgcg agacccgatt ctgatcagcg cttctgcaca cagtatatca 2281 tctatgaaga tttgtgtaat tatcacccag agagggttcg gcagggcaaa gtctggaagg 2341 ctccttcgag ctggtttata gactgtgtga tgtcctttga gttgcttcct cttgacagct 2401 nnccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 2461 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 2521 ggatta