Construct: ORF ccsbBroad304_15412

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011272.1_s304c1, BRDN0000407590
Derived from:
ccsbBroadEn_15412
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EWSR1 (2130)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15412
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2130 EWSR1 EWS RNA binding protein 1 NM_001163285.2 99.9% 100% 480G>A
2 human 2130 EWSR1 EWS RNA binding protein 1 NM_005243.4 99.7% 99.8% 480G>A;974_976delGCA
3 human 2130 EWSR1 EWS RNA binding protein 1 XM_011529998.2 99.7% 99.8% 100_102delCAG;483G>A
4 human 2130 EWSR1 EWS RNA binding protein 1 XM_011529997.2 99.6% 99.6% 100_102delCAG;483G>A;977_979delGCA
5 human 2130 EWSR1 EWS RNA binding protein 1 NM_013986.4 99% 99% 226_243del;498G>A
6 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028644.2 98% 98% 480G>A;1578_1616del
7 human 2130 EWSR1 EWS RNA binding protein 1 XM_005261389.4 97.8% 97.9% 480G>A;974_976delGCA;1581_1619del
8 human 2130 EWSR1 EWS RNA binding protein 1 XM_011529996.3 97.8% 97.9% 100_102delCAG;483G>A;1581_1619del
9 human 2130 EWSR1 EWS RNA binding protein 1 XM_011529995.3 97.7% 97.7% (many diffs)
10 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028648.2 97.3% 97.4% 480G>A;1624_1625ins51
11 human 2130 EWSR1 EWS RNA binding protein 1 XM_011530000.2 97% 97.1% (many diffs)
12 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028647.2 95.4% 95.5% 480G>A;1578_1616del;1663_1664ins51
13 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028645.2 95.3% 95.3% (many diffs)
14 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028646.2 95.3% 95.3% (many diffs)
15 human 2130 EWSR1 EWS RNA binding protein 1 XM_011529999.3 95.1% 95.2% (many diffs)
16 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028653.2 93.6% 93.7% 480G>A;1290_1291ins123
17 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028651.2 93.5% 93.5% 480G>A;974_976delGCA;1293_1294ins123
18 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028652.2 93.5% 93.5% 100_102delCAG;483G>A;1293_1294ins123
19 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028650.2 93.4% 93.4% (many diffs)
20 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028649.2 91.5% 91.6% (many diffs)
21 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028659.1 91.4% 91.4% 411_412ins168
22 human 2130 EWSR1 EWS RNA binding protein 1 NM_001163286.2 91.3% 91.3% 411_412ins168;806_808delGCA
23 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028658.1 91.3% 91.3% 100_102delCAG;414_415ins168
24 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028660.2 91% 91.1% 480G>A;1290_1291ins123;1501_1502ins51
25 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028655.1 89.6% 89.6% 411_412ins168;1410_1448del
26 human 2130 EWSR1 EWS RNA binding protein 1 XM_005261390.4 89.5% 89.5% 411_412ins168;806_808delGCA;1413_1451del
27 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028654.1 89.5% 89.5% 100_102delCAG;414_415ins168;1413_1451del
28 human 2130 EWSR1 EWS RNA binding protein 1 XM_011530001.2 89.4% 89.4% (many diffs)
29 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028661.2 89.2% 88.7% (many diffs)
30 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028656.2 89.1% 89.2% (many diffs)
31 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028662.2 88.8% 88.8% 411_412ins168;1456_1457ins51
32 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028657.2 87.3% 86.8% (many diffs)
33 human 2130 EWSR1 EWS RNA binding protein 1 XM_011530002.3 87% 86.9% (many diffs)
34 human 2130 EWSR1 EWS RNA binding protein 1 XM_024452181.1 86.3% 86.2% 480G>A;793_794ins216;1408_1409ins51
35 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028663.1 85.1% 85.1% 411_412ins168;1122_1123ins123
36 human 2130 EWSR1 EWS RNA binding protein 1 XM_024452180.1 84.5% 84.4% (many diffs)
37 human 2130 EWSR1 EWS RNA binding protein 1 NM_001163287.2 52.3% 48% (many diffs)
38 human 2130 EWSR1 EWS RNA binding protein 1 XR_002958676.1 50% (many diffs)
39 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028665.2 48.3% 48.3% 0_1ins1014
40 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028666.2 48.3% 48.3% 0_1ins1014
41 human 2130 EWSR1 EWS RNA binding protein 1 XM_017028664.2 47.4% 47.4% 0_1ins1014;564_602del
42 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... NM_007968.3 93.4% 97.8% (many diffs)
43 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... NM_001283061.1 93.3% 97.7% (many diffs)
44 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... XM_006514512.3 92.6% 96.9% (many diffs)
45 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... XM_017314255.1 92.6% 96.9% (many diffs)
46 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... XM_006514510.2 92.4% 96.8% (many diffs)
47 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... XM_006514511.2 92.4% 96.8% (many diffs)
48 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... XM_006514509.2 91.8% 96.1% (many diffs)
49 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... XM_006514508.2 91.6% 95.9% (many diffs)
50 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... NM_001283062.1 88% 92.6% (many diffs)
51 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... XR_001779874.1 73.8% (many diffs)
52 mouse 14030 Ewsr1 Ewing sarcoma breakpoint re... NM_001283063.1 46.7% 48% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2031
ORF length:
1965
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gtccacggat tacagtacct atagccaagc tgcagcgcag cagggctaca
121gtgcttacac cgcccagccc actcaaggat atgcacagac cacccaggca tatgggcaac
181aaagctatgg aacctatgga cagcccactg atgtcagcta tacccaggct cagaccactg
241caacctatgg gcagaccgcc tatgcaactt cttatggaca gcctcccact ggttatacta
301ctccaactgc cccccaggca tacagccagc ctgtccaggg gtatggcact ggtgcttatg
361ataccaccac tgctacagtc accaccaccc aggcctccta tgcagctcag tctgcatatg
421gcactcagcc tgcttatcca gcctatgggc agcagccagc agccactgca cctacaagac
481cgcaggatgg aaacaagccc actgagacta gtcaacctca atctagcaca gggggttaca
541accaacccag cctaggatat ggacagagta actacagtta tccccaggta cctgggagct
601accccatgca gccagtcact gcacctccat cctaccctcc taccagctat tcctctacac
661agccgactag ttatgatcag agcagttact ctcagcagaa cacctatggg caaccgagca
721gctatggaca gcagagtagc tatggtcaac aaagcagcta tgggcagcag cctcccacta
781gttacccacc ccaaactgga tcctacagcc aagctccaag tcaatatagc caacagagca
841gcagctacgg gcagcagagt tcattccgac aggaccaccc cagtagcatg ggtgtttatg
901ggcaggagtc tggaggattt tccggaccag gagagaaccg gagcatgagt ggccctgata
961accggggcag gggaagaggg ggatttgatc gtggaggcat gagcagaggt gggcggggag
1021gaggacgcgg tggaatgggc gctggagagc gaggtggctt caataagcct ggtggaccca
1081tggatgaagg accagatctt gatctaggcc cacctgtaga tccagatgaa gactctgaca
1141acagtgcaat ttatgtacaa ggattaaatg acagtgtgac tctagatgat ctggcagact
1201tctttaagca gtgtggggtt gttaagatga acaagagaac tgggcaaccc atgatccaca
1261tctacctgga caaggaaaca ggaaagccca aaggcgatgc cacagtgtcc tatgaagacc
1321cacccactgc caaggctgcc gtggaatggt ttgatgggaa agattttcaa gggagcaaac
1381ttaaagtctc ccttgctcgg aagaagcctc caatgaacag tatgcggggt ggtctgccac
1441cccgtgaggg cagaggcatg ccaccaccac tccgtggagg tccaggaggc ccaggaggtc
1501ctgggggacc catgggtcgc atgggaggcc gtggaggaga tagaggaggc ttccctccaa
1561gaggaccccg gggttcccga gggaacccct ctggaggagg aaacgtccag caccgagctg
1621gagactggca gtgtcccaat ccgggttgtg gaaaccagaa cttcgcctgg agaacagagt
1681gcaaccagtg taaggcccca aagcctgaag gcttcctccc gccacccttt ccgcccccgg
1741gtggtgatcg tggcagaggt ggccctggtg gcatgcgggg aggaagaggt ggcctcatgg
1801atcgtggtgg tcccggtgga atgttcagag gtggccgtgg tggagacaga ggtggcttcc
1861gtggtggccg gggcatggac cgaggtggct ttggtggagg aagacgaggt ggccctgggg
1921ggccccctgg acctttgatg gaacagatgg gaggaagaag aggaggacgt ggaggacctg
1981gaaaaatgga taaaggcgag caccgtcagg agcgcagaga tcggccctac tnnccaactt
2041tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
2101agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)