Construct: ORF ccsbBroad304_15441

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011379.1_s304c1, BRDN0000404134
Derived from:
ccsbBroadEn_15441
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HGF (3082)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15441
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3082 HGF hepatocyte growth factor NM_001010933.3 100% 100%
2 human 3082 HGF hepatocyte growth factor XM_017012098.1 100% 100%
3 human 3082 HGF hepatocyte growth factor NM_001010931.3 98.2% 98.2% 481_495del
4 human 3082 HGF hepatocyte growth factor XM_017012097.1 98.2% 98.2% 481_495del
5 human 3082 HGF hepatocyte growth factor NM_001010934.3 70.4% 70% (many diffs)
6 human 3082 HGF hepatocyte growth factor NM_001010932.3 39.3% 39.1% 851_852delCTinsA;857_2169del
7 human 3082 HGF hepatocyte growth factor XM_011516115.2 39.3% 39.1% 851_852delCTinsA;857_2169del
8 human 3082 HGF hepatocyte growth factor NM_000601.6 39.1% 38.8% 481_495del;866_867delCTinsA;872_2184del
9 human 3082 HGF hepatocyte growth factor XM_006715956.2 39.1% 38.8% 481_495del;866_867delCTinsA;872_2184del
10 mouse 15234 Hgf hepatocyte growth factor NM_001289460.2 62% 62.1% (many diffs)
11 mouse 15234 Hgf hepatocyte growth factor NM_001289461.1 35.1% 35.5% (many diffs)
12 mouse 15234 Hgf hepatocyte growth factor NM_001289458.1 34.9% 35.3% (many diffs)
13 mouse 15234 Hgf hepatocyte growth factor NM_001289459.1 34.9% 35.3% (many diffs)
14 mouse 15234 Hgf hepatocyte growth factor NM_010427.5 34.9% 35.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
921
ORF length:
855
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtg ggtgaccaaa ctcctgccag ccctgctgct gcagcatgtc ctcctgcatc
121tcctcctgct ccccatcgcc atcccctatg cagagggaca aaggaaaaga agaaatacaa
181ttcatgaatt caaaaaatca gcaaagacta ccctaatcaa aatagatcca gcactgaaga
241taaaaaccaa aaaagtgaat actgcagacc aatgtgctaa tagatgtact aggaataaag
301gacttccatt cacttgcaag gcttttgttt ttgataaagc aagaaaacaa tgcctctggt
361tccccttcaa tagcatgtca agtggagtga aaaaagaatt tggccatgaa tttgacctct
421atgaaaacaa agactacatt agaaactgca tcattggtaa aggacgcagc tacaagggaa
481cagtatctat cactaagagt ggcatcaaat gtcagccctg gagttccatg ataccacacg
541aacacagcta tcggggtaaa gacctacagg aaaactactg tcgaaatcct cgaggggaag
601aagggggacc ctggtgtttc acaagcaatc cagaggtacg ctacgaagtc tgtgacattc
661ctcagtgttc agaagttgaa tgcatgacct gcaatgggga gagttatcga ggtctcatgg
721atcatacaga atcaggcaag atttgtcagc gctgggatca tcagacacca caccggcaca
781aattcttgcc tgaaagatat cccgacaagg gctttgatga taattattgc cgcaatcccg
841atggccagcc gaggccatgg tgctatactc ttgaccctca cacccgctgg gagtactgtg
901caattaaaac atgcgagaca tnnccaactt tcttgtacaa agtggttggt aagcctatcc
961ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1021ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)