Construct: ORF ccsbBroad304_15459
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013406.1_s304c1, BRDN0000406564
- Derived from:
- ccsbBroadEn_15459
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ICA1 (3382)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15459
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350838.2 | 65.7% | 54.4% | (many diffs) |
| 2 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350831.2 | 65.2% | 60.2% | (many diffs) |
| 3 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350832.2 | 65.2% | 60.2% | (many diffs) |
| 4 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350833.2 | 65.2% | 60.2% | (many diffs) |
| 5 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350834.2 | 65% | 60% | (many diffs) |
| 6 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350830.2 | 63.2% | 54% | (many diffs) |
| 7 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001136020.3 | 62.8% | 57.2% | (many diffs) |
| 8 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350819.2 | 62.8% | 57.2% | (many diffs) |
| 9 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350820.2 | 62.8% | 57.2% | (many diffs) |
| 10 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_004968.3 | 62.8% | 57.2% | (many diffs) |
| 11 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_022307.3 | 62.8% | 57.2% | (many diffs) |
| 12 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001276478.2 | 62.6% | 56.9% | (many diffs) |
| 13 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350821.2 | 62.6% | 56.9% | (many diffs) |
| 14 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350823.2 | 62.6% | 56.9% | (many diffs) |
| 15 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350824.2 | 62.6% | 56.9% | (many diffs) |
| 16 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350825.2 | 62.6% | 56.9% | (many diffs) |
| 17 | human | 3382 | ICA1 | islet cell autoantigen 1 | XR_002956427.1 | 61.5% | 1_160del;866_962del;1045_1046ins235 | |
| 18 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350826.2 | 60% | 52.1% | (many diffs) |
| 19 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350827.2 | 60% | 52.1% | (many diffs) |
| 20 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_011515347.3 | 60% | 52.1% | (many diffs) |
| 21 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_011515348.1 | 60% | 52.1% | (many diffs) |
| 22 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_011515349.1 | 60% | 52.1% | (many diffs) |
| 23 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350828.2 | 59.8% | 51.9% | (many diffs) |
| 24 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350829.2 | 59.8% | 51.9% | (many diffs) |
| 25 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_011515351.1 | 59.8% | 51.9% | (many diffs) |
| 26 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_011515353.2 | 59.8% | 51.9% | (many diffs) |
| 27 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_017012116.1 | 59.8% | 51.9% | (many diffs) |
| 28 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_024446741.1 | 59.8% | 51.9% | (many diffs) |
| 29 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_024446740.1 | 54.2% | 47.1% | (many diffs) |
| 30 | human | 3382 | ICA1 | islet cell autoantigen 1 | XR_002956426.1 | 51.8% | (many diffs) | |
| 31 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350835.2 | 47.4% | 39.9% | (many diffs) |
| 32 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350836.2 | 47.4% | 39.9% | (many diffs) |
| 33 | human | 3382 | ICA1 | islet cell autoantigen 1 | NM_001350837.2 | 47.4% | 39.9% | (many diffs) |
| 34 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_017012125.1 | 47.4% | 39.9% | (many diffs) |
| 35 | human | 3382 | ICA1 | islet cell autoantigen 1 | XM_011515354.1 | 45.5% | 36.4% | (many diffs) |
| 36 | human | 3382 | ICA1 | islet cell autoantigen 1 | NR_146927.2 | 42% | (many diffs) | |
| 37 | human | 3382 | ICA1 | islet cell autoantigen 1 | NR_146929.2 | 38.6% | (many diffs) | |
| 38 | human | 3382 | ICA1 | islet cell autoantigen 1 | NR_146926.2 | 38.5% | (many diffs) | |
| 39 | human | 3382 | ICA1 | islet cell autoantigen 1 | NR_146928.2 | 37.5% | (many diffs) | |
| 40 | mouse | 15893 | Ica1 | islet cell autoantigen 1 | NM_001252266.1 | 62.4% | 56.3% | (many diffs) |
| 41 | mouse | 15893 | Ica1 | islet cell autoantigen 1 | XM_006504995.3 | 62.4% | 56.3% | (many diffs) |
| 42 | mouse | 15893 | Ica1 | islet cell autoantigen 1 | NM_010492.3 | 60.5% | 54% | (many diffs) |
| 43 | mouse | 15893 | Ica1 | islet cell autoantigen 1 | XM_006504993.3 | 60.5% | 54% | (many diffs) |
| 44 | mouse | 15893 | Ica1 | islet cell autoantigen 1 | XM_006504994.3 | 60.5% | 54% | (many diffs) |
| 45 | mouse | 15893 | Ica1 | islet cell autoantigen 1 | XR_001785099.1 | 30% | (many diffs) | |
| 46 | mouse | 15893 | Ica1 | islet cell autoantigen 1 | XR_001785098.1 | 29.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1089
- ORF length:
- 1023
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc aggacacaaa tgcagttatc cctgggactt acaggatcga tatgctcaag 121 ataagtcagt tgtaaataag atgcaacaga aatattggga gacgaagcag gcctttatta 181 aagccacagg gaagaaggaa gatgaacatg ttgttgcctc tgacgcggac ctggatgcca 241 agctagagct gtttcattca attcagagaa cctgtctgga cttatcgaaa gcaattgtac 301 tctatcaaaa gaggatatgt ttcttgtctc aagaagaaaa cgaactggga aaatttcttc 361 gatcccaagg tttccaagat aaaaccagag caggaaagat gatgcaagcg acaggaaagg 421 ccctctgctt ttcttcccag caaaggttgg ccttacgaaa tcctttgtgt cgatttcacc 481 aagaagtgga gacttttcgg catcgggcca tctcagatac ttggctgacg gtgaaccgca 541 tggaacagtg caggacggaa tatagaggag cactattatg gatgaaggac gtgtctcagg 601 agcttgatcc agacctctac aagcaaatgg agaagttcag gaaggtacaa acacaagtgc 661 gccttgcaaa aaaaaacttt gacaaattga agatggatgt ttgtcaaaaa gtggatcttc 721 ttggagcgag cagatgcaat ctcttgtctc acatgctagc aacataccag accactctgc 781 ttcatttttg ggagaaaact tctcacacta tggcagccat ccatgagagt ttcaaaggtt 841 atcaaccata tgaatttact actttaaagg tatggaagct agaggggcca ctagaaaatc 901 ttgagaaaag taaagttttg tttatatcac aagcaaagtc cattgcagaa agcatcatta 961 ttccaggact cagaatgctg aagagagaac tgtgccccca gcagagtctt tttctttctt 1021 tctttctttt tttttactgc atcaggattc aatacaatga gaatctactg caatccaaaa 1081 gcgcgaattn nccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1141 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1201 tcaacctctg gatta