Construct: ORF ccsbBroad304_15559

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007325.1_s304c1, BRDN0000407803
Derived from:
ccsbBroadEn_15559
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RAC1 (5879)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15559
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5879 RAC1 Rac family small GTPase 1 NM_006908.5 100% 100%
2 human 5879 RAC1 Rac family small GTPase 1 NM_018890.4 90.9% 90.9% 226_282del
3 human 5880 RAC2 Rac family small GTPase 2 NM_002872.5 76.3% 92.1% (many diffs)
4 human 5881 RAC3 Rac family small GTPase 3 NM_005052.3 76% 92.7% (many diffs)
5 mouse 19353 Rac1 RAS-related C3 botulinum su... NM_009007.2 91.6% 100% (many diffs)
6 mouse 19353 Rac1 RAS-related C3 botulinum su... NM_001347530.1 83.4% 90.9% (many diffs)
7 mouse 102631998 Gm30181 predicted gene, 30181 XR_384352.1 55.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
642
ORF length:
576
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca ggccatcaag tgtgtggtgg tgggagacgg agctgtaggt aaaacttgcc
121tactgatcag ttacacaacc aatgcatttc ctggagaata tatccctact gtctttgaca
181attattctgc caatgttatg gtagatggaa aaccggtgaa tctgggctta tgggatacag
241ctggacaaga agattatgac agattacgcc ccctatccta tccgcaaaca gatgtgttct
301taatttgctt ttcccttgtg agtcctgcat catttgaaaa tgtccgtgca aagtggtatc
361ctgaggtgcg gcaccactgt cccaacactc ccatcatcct agtgggaact aaacttgatc
421ttagggatga taaagacacg atcgagaaac tgaaggagaa gaagctgact cccatcacct
481atccgcaggg tctagccatg gctaaggaga ttggtgctgt aaaatacctg gagtgctcgg
541cgctcacaca gcgaggcctc aagacagtgt ttgacgaagc gatccgagca gtcctctgcc
601cgcctcccgt gaagaagagg aagagaaaat gcctgctgtt gtnnccaact ttcttgtaca
661aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
721ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)