Construct: ORF ccsbBroad304_15587
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017462.1_s304c1, BRDN0000407897
- Derived from:
- ccsbBroadEn_15587
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHMT1 (6470)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15587
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | NM_148918.2 | 100% | 100% | |
| 2 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | XM_017024958.1 | 100% | 100% | |
| 3 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | NM_004169.5 | 91.9% | 91.9% | 814_930del |
| 4 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | XM_005256767.3 | 91.9% | 91.9% | 814_930del |
| 5 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | XM_017024957.1 | 91.9% | 91.9% | 814_930del |
| 6 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | XM_011523992.3 | 90.7% | 90.7% | 812_813ins123 |
| 7 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | XM_024450887.1 | 90.7% | 90.7% | 812_813ins123 |
| 8 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | XM_024450889.1 | 68.9% | 68.9% | 0_1ins414 |
| 9 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | NM_001281786.1 | 63.3% | 63.3% | 0_1ins414;400_516del |
| 10 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | XM_024450888.1 | 63.3% | 63.3% | 0_1ins414;400_516del |
| 11 | human | 6470 | SHMT1 | serine hydroxymethyltransfe... | XM_024450890.1 | 59.6% | 59.6% | 0_1ins414;398_399ins123 |
| 12 | mouse | 20425 | Shmt1 | serine hydroxymethyltransfe... | NM_009171.2 | 77.7% | 82.8% | (many diffs) |
| 13 | mouse | 20425 | Shmt1 | serine hydroxymethyltransfe... | XM_006532644.3 | 77.7% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac gatgccagtc aacggggccc acaaggatgc tgacctgtgg tcctcacatg 121 acaagatgct ggcacaaccc ctcaaagaca gtgatgttga ggtttacaac atcattaaga 181 aggagagtaa ccggcagagg gttggattgg agctgattgc ctcggagaat ttcgccagcc 241 gagcagtttt ggaggcccta ggctcttgct taaataacaa atactctgag gggtacccgg 301 gccagagata ctatggcggg actgagttta ttgatgaact ggagaccctc tgtcagaagc 361 gagccctgca ggcctataag ctggacccac agtgctgggg ggtcaacgtc cagccctact 421 caggctcccc tgcaaacttt gctgtgtaca ctgccctggt ggaaccccat gggcgcatca 481 tgggcctgga ccttccggat gggggccacc tgacccatgg gttcatgaca gacaagaaga 541 aaatctctgc cacgtccatc ttctttgaat ctatgcccta caaggtgaac ccagatactg 601 gctacatcaa ctatgaccag ctggaggaga acgcacgcct cttccacccg aagctgatca 661 tcgcaggaac cagctgctac tcccgaaacc tggaatatgc ccggctacgg aagattgcag 721 atgagaacgg ggcgtatctc atggcggaca tggctcacat cagcgggctg gtggcggctg 781 gcgtggtgcc ctccccattt gaacactgcc atgtggtgac caccaccact cacaagaccc 841 tgcgaggctg ccgagctggc atgatcttct acaggaaagg ggttgctgtg gcactgaagc 901 aagctatgac tctggaattt aaagtttatc aacaccaggt ggtggccaac tgcagggctc 961 tgtctgaggc cctgacggag ctgggctaca aaatagtcac aggtggttct gacaaccatt 1021 tgatccttgt ggatctccgt tccaaaggca cagatggtgg aagggctgag aaggtgctag 1081 aagcctgttc tattgcctgc aacaagaaca cctgtccagg tgacagaagc gctctgcggc 1141 ccagtggact gcggctgggg accccagcac tgacgtcccg tggacttttg gaaaaagact 1201 tccaaaaagt agcccacttt attcacagag ggatagagct gaccctgcag atccagagcg 1261 acactggtgt cagagccacc ctgaaagagt tcaaggagag actggcaggg gataagtacc 1321 aggcggccgt gcaggctctc cgggaggagg ttgagagctt cgcctctctc ttccctctgc 1381 ctggcctgcc tgacttctnn ccaactttct tgtacaaagt ggttggtaag cctatcccta 1441 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1501 agtcgacaat caacctctgg atta