Transcript: Human NM_148918.2

Homo sapiens serine hydroxymethyltransferase 1 (SHMT1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
SHMT1 (6470)
Length:
2436
CDS:
212..1546

Additional Resources:

NCBI RefSeq record:
NM_148918.2
NBCI Gene record:
SHMT1 (6470)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_148918.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000034764 GCAAGCTATGACTCTGGAATT pLKO.1 1045 CDS 100% 0.000 0.000 N SHMT1 n/a
2 TRCN0000034766 CCTAGGCTCTTGCTTAAATAA pLKO.1 403 CDS 100% 15.000 10.500 N SHMT1 n/a
3 TRCN0000034765 CCCAGATACTGGCTACATCAA pLKO.1 736 CDS 100% 4.950 3.465 N SHMT1 n/a
4 TRCN0000034768 CTTGTGGATCTCCGTTCCAAA pLKO.1 1172 CDS 100% 4.950 3.465 N SHMT1 n/a
5 TRCN0000034767 CCACTTTATTCACAGAGGGAT pLKO.1 1360 CDS 100% 2.640 1.848 N SHMT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_148918.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15587 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15587 pLX_304 0% 100% 100% V5 n/a
3 ccsbBroadEn_06946 pDONR223 100% 91.8% 91.7% None 813_814ins117;1303C>T n/a
4 ccsbBroad304_06946 pLX_304 0% 91.8% 91.7% V5 813_814ins117;1303C>T n/a
5 TRCN0000480756 ACAGGATACATGATTACATGCCCC pLX_317 26.4% 91.8% 91.7% V5 813_814ins117;1303C>T n/a
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