Construct: ORF ccsbBroad304_15593
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017234.1_s304c1, BRDN0000404411
- Derived from:
- ccsbBroadEn_15593
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC4A2 (6522)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15593
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | NM_001199694.2 | 88.5% | 86.7% | (many diffs) |
| 2 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | NM_001199693.1 | 88.1% | 86.4% | (many diffs) |
| 3 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | NM_001199692.2 | 87.5% | 85.8% | (many diffs) |
| 4 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | NM_003040.4 | 87.5% | 85.8% | (many diffs) |
| 5 | human | 6522 | SLC4A2 | solute carrier family 4 mem... | XM_006716094.3 | 41.2% | 39.1% | (many diffs) |
| 6 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535656.3 | 77.8% | 82.6% | (many diffs) |
| 7 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535655.3 | 77.5% | 82.2% | (many diffs) |
| 8 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | NM_001253892.1 | 77% | 81.6% | (many diffs) |
| 9 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | NM_009207.3 | 77% | 81.6% | (many diffs) |
| 10 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535650.1 | 77% | 81.6% | (many diffs) |
| 11 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535651.3 | 77% | 81.6% | (many diffs) |
| 12 | mouse | 20535 | Slc4a2 | solute carrier family 4 (an... | XM_006535653.1 | 76.7% | 81.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3327
- ORF length:
- 3261
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gaggaagact ttgagtacca ccgccagtcc tcccaccaca tccatcaccc 121 actgtccacc actgccccac caggaggcga ctcctcgggc ctccaaaggg gcccaggctg 181 gaacccaggt ggaggaggcg gaggcggagg cggtggcggt ggccagtggc actgcagggg 241 gtgacgacgg gggtgcctcg gggcgccccc tgcccaaagc ccagcctggg caccgcagct 301 acaaccttca ggagaggagg cgcatcggga gcatgactgg ggctgagcag gcactgctgc 361 cccgggtccc cacggatgag attgaggccc agacgctggc cacggccgac ctagacctca 421 tgaagagtca ccggtttgag gacgttcctg gggtgcggcg gcacttggtg cggaagaatg 481 ccaaaggttc cacacagagt ggccgagaag ggcgggagcc tggccccaca cctcgggccc 541 gaccccgggc cccccacaag ccccatgagg tgtttgtgga gctgaatgag ttgctcctgg 601 acaaaaacca ggagccccag tggcgggaga cagctcgctg gatcaaattt gaagaggacg 661 tggaggagga gaccgagcgc tgggggaagc cccacgtggc ctccctctcc ttccgcagtc 721 tcctggagct ccgcaggacc ctggcccatg gggctgtgct cttggatctg gaccagcaga 781 ccctgcccgg agtggcccac caggtggtgg agcagatggt catctctgac cagatcaagg 841 ccgaggacag ggccaacgtg ctgcgggctc tgctgttgaa acacagccac ccaagtgatg 901 agaaggactt ctccttcccc cgcaacatct cagctggctc cctgggctcc ctgctggggc 961 atcaccatgg tcagggggct gagagtgacc cccacgtcac cgagcctctc atgggaggtg 1021 ttcctgagac ccggctggag gtggagcgag agcgtgagct gccgcctcca gcaccaccag 1081 ctggcatcac ccgctccaag tccaagcacg agctgaaact gctggagaag attcctgaga 1141 atgccgaggc cacggtggtc cttgtgggct gcgtggagtt cctctcccgc cccaccatgg 1201 cctttgtgcg gctccgggag gctgtggagt tggacgcagt gttggaggtg ccggtgcctg 1261 tgcgtttcct cttcctgctg ctgggcccga gtagtgccaa catggactac cacgagatcg 1321 gccgctccat ctccaccctc atgtcagaca agcaattcca cgaggcagcc tacctggctg 1381 acgagcggga ggacctgctg acggccatca acgccttcct ggactgcagc gtggtgctgc 1441 cgccttcaga agtgcagggc gaggagctgc tgcgctctgt ggcccacttc cagcgccaga 1501 tgctcaagaa gcgagaggag cagggccggc tgctacctac aggggctggg ctggagccca 1561 aatctgccca agataaggcg ctcctgcaga tggtagaggc ggcaggggca gctgaagatg 1621 atccccttcg gcggacgggg cggccctttg gggggctgat ccgagatgtg cggcgccgct 1681 atccccacta cctgagtgac ttccgagatg cacttgaccc tcagtgcctg gccgcagtca 1741 tcttcatcta ctttgccgcc ctgtctcctg ccatcacctt tggggggctg ctgggagaga 1801 agacacagga cctgataggg gtgtcggagc tgattatgtc cacagcgctc cagggcgtgg 1861 tcttctgcct gctgggtgcc cagcccctgt tggtgatcgg cttctcaggg cccctgctgg 1921 tctttgagga ggccttcttc tcgttctgta gcagcaacca cctggagtac ctggtgggcc 1981 gtgtgtggat cggcttctgg ctggtgttcc tggccctgct catggtggcc ctggagggga 2041 gcttcctggt ccgcttcgtc tcccgcttca cccaggagat cttcgccttc ttgatctcac 2101 tcatcttcat ctatgagacc ttctacaagc tggtgaagat cttccaggag caccccctgc 2161 atggctgctc agcctccaac agctcagagg tggacggcgg tgagaacatg acatgggccg 2221 gggcaagacc cacgctgggg ccgggcaaca ggagcttggc tgggcagtct gggcagggga 2281 agccccgggg ccagcccaac acggccctgc tgtcgctggt gctcatggcc ggcaccttct 2341 tcatcgcctt cttcctgcgc aaattcaaga acagccggtt ctttcctggc cggatccggc 2401 gggtgattgg ggactttggg gtgcccatcg ccatcctcat catggtgctt gtggattaca 2461 gtattgagga cacctatacc cagaagctga gcgttcccag tggattctcg gtgactgccc 2521 cagaaaagag gggctgggtc atcaaccccc tgggagagaa gagccccttc cctgtgtgga 2581 tgatggttgc cagcctgctg cccgccatcc tggtcttcat tctcatcttc atggagacac 2641 agatcaccac gctcatcatc tccaagaagg agcgcatgct gcagaagggc tccggcttcc 2701 acctggacct gctgctcatc gtggccatgg gcggcatctg tgccctcttt ggcctgccct 2761 ggttggctgc tgccactgtc cgctctgtca ctcacgccaa cgcgctcact gtcatgagca 2821 aggctgtggc acctggggac aagcccaaga ttcaggaagt caaggagcag cgggtgacgg 2881 ggctgctggt tgccctgctt gtgggcctct ccatagttat cggggatctg ctccggcaga 2941 tccccctggc cgtgctcttt ggaattttcc tgtacatggg agtcacctcc cttaacggga 3001 tccagttcta tgagcggctg catctgctgc tcatgccgcc caaacaccac ccagatgtca 3061 cttacgtcaa gaaggtccgg accctccgta tgcacctgtt cacggccctg cagctgctct 3121 gcctggccct gctctgggcc gtcatgtcca cagctgcctc cctggccttc cccttcatcc 3181 tcatcctcac agtgccgctc cgcatggtgg tgctcacccg tatcttcacc gaccgagaga 3241 tgaaatgtct ggatgctaac gaggcagagc cggtgtttga tgagcgggag ggtgtggacg 3301 agtacaatga gatgcccatg cctgtgtnnc caactttctt gtacaaagtg gttggtaagc 3361 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 3421 ggcgcgttaa gtcgacaatc aacctctgga tta