Construct: ORF ccsbBroad304_15620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011860.1_s304c1, BRDN0000403266
- Derived from:
- ccsbBroadEn_15620
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZAP70 (7535)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15620
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7535 | ZAP70 | zeta chain of T cell recept... | NM_001079.3 | 79.3% | 78.5% | (many diffs) |
| 2 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004867.1 | 66.2% | 65.4% | (many diffs) |
| 3 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004868.1 | 65.4% | 64.6% | (many diffs) |
| 4 | human | 7535 | ZAP70 | zeta chain of T cell recept... | NM_207519.1 | 63.2% | 63.2% | 0_1ins543 |
| 5 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004869.1 | 62.3% | 60.7% | (many diffs) |
| 6 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XM_017004870.1 | 61.4% | 59.9% | (many diffs) |
| 7 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XR_001738927.1 | 39.1% | (many diffs) | |
| 8 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XR_001738926.1 | 33.3% | (many diffs) | |
| 9 | human | 7535 | ZAP70 | zeta chain of T cell recept... | XR_001738925.1 | 29.7% | (many diffs) | |
| 10 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_001289765.1 | 69.3% | 72.5% | (many diffs) |
| 11 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_009539.3 | 69.3% | 72.5% | (many diffs) |
| 12 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | XM_006495896.2 | 69.3% | 72.5% | (many diffs) |
| 13 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_001289766.1 | 66.8% | 69.8% | (many diffs) |
| 14 | mouse | 22637 | Zap70 | zeta-chain (TCR) associated... | NM_001289612.1 | 55.3% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1545
- ORF length:
- 1479
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg cttggggccg cgctggaagg gcgaggccct ggagcaggcc atcatcagcc 121 aggccccgca ggtggagaag ctcattgcta cgacggccca cgagcggatg ccctggtacc 181 acagcagcct gacgcgtgag gaggccgagc gcaaacttta ctctggggcg cagaccgacg 241 gcaagttcct gctgaggccg cggaaggagc agggcacata cgccctgtcc ctcatctatg 301 ggaagacggt gtaccactac ctcatcagcc aagacaaggc gggcaagtac tgcattcccg 361 agggcaccaa gtttgacacg ctctggcagc tggtggagta tctgaagctg aaggcggacg 421 ggctcatcta ctgcctgaag gaggcctgcc ccaacagcag tgccagcaac gcctcagggg 481 ctgctgctcc cacactccca gcccacccat ccacgttgac tcatcctcag agacgaatcg 541 acaccctcaa ctcagatgga tacacccctg agccagcacg cataacgtcc ccagacaaac 601 cgcggccgat gcccatggac acgagcgtgt atgagagccc ctacagcgac ccagaggagc 661 tcaaggacaa gaagctcttc ctgaagcgcg ataacctcct catagctgac attgaacttg 721 gctgcggcaa ctttggctca gtgcgccagg gcgtgtaccg catgcgcaag aagcagatcg 781 acgtggccat caaggtgctg aagcagggca cggagaaggc agacacggaa gagatgatgc 841 gcgaggcgca gatcatgcac cagctggaca acccctacat cgtgcggctc attggcgtct 901 gccaggccga ggccctcatg ctggtcatgg agatggctgg gggcgggccg ctgcacaagt 961 tcctggtcgg caagagggag gagatccctg tgagcaatgt ggccgagctg ctgcaccagg 1021 tgtccatggg gatgaagtac ctggaggaga agaactttgt gcaccgtgac ctggcggccc 1081 gcaacgtcct gctggttaac cggcactacg ccaagatcag cgactttggc ctctccaaag 1141 cactgggtgc cgacgacagc tactacactg cccgctcagc agggaagtgg ccgctcaagt 1201 ggtacgcacc cgaatgcatc aacttccgca agttctccag ccgcagcgat gtctggagct 1261 atggggtcac catgtgggag gccttgtcct acggccagaa gccctacaag aagatgaaag 1321 ggccggaggt catggccttc atcgagcagg gcaagcggat ggagtgccca ccagagtgtc 1381 cacccgaact gtacgcactc atgagtgact gctggatcta caagtgggag gatcgccccg 1441 acttcctgac cgtggagcag cgcatgcgag cctgttacta cagcctggcc agcaaggtgg 1501 aagggccccc aggcagcaca cagaaggctg aggctgcctg tgcctnncca actttcttgt 1561 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1621 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a