Construct: ORF ccsbBroad304_15620

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011860.1_s304c1, BRDN0000403266
Derived from:
ccsbBroadEn_15620
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ZAP70 (7535)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15620
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7535 ZAP70 zeta chain of T cell recept... NM_001079.3 79.3% 78.5% (many diffs)
2 human 7535 ZAP70 zeta chain of T cell recept... XM_017004867.1 66.2% 65.4% (many diffs)
3 human 7535 ZAP70 zeta chain of T cell recept... XM_017004868.1 65.4% 64.6% (many diffs)
4 human 7535 ZAP70 zeta chain of T cell recept... NM_207519.1 63.2% 63.2% 0_1ins543
5 human 7535 ZAP70 zeta chain of T cell recept... XM_017004869.1 62.3% 60.7% (many diffs)
6 human 7535 ZAP70 zeta chain of T cell recept... XM_017004870.1 61.4% 59.9% (many diffs)
7 human 7535 ZAP70 zeta chain of T cell recept... XR_001738927.1 39.1% (many diffs)
8 human 7535 ZAP70 zeta chain of T cell recept... XR_001738926.1 33.3% (many diffs)
9 human 7535 ZAP70 zeta chain of T cell recept... XR_001738925.1 29.7% (many diffs)
10 mouse 22637 Zap70 zeta-chain (TCR) associated... NM_001289765.1 69.3% 72.5% (many diffs)
11 mouse 22637 Zap70 zeta-chain (TCR) associated... NM_009539.3 69.3% 72.5% (many diffs)
12 mouse 22637 Zap70 zeta-chain (TCR) associated... XM_006495896.2 69.3% 72.5% (many diffs)
13 mouse 22637 Zap70 zeta-chain (TCR) associated... NM_001289766.1 66.8% 69.8% (many diffs)
14 mouse 22637 Zap70 zeta-chain (TCR) associated... NM_001289612.1 55.3% 59% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1545
ORF length:
1479
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg cttggggccg cgctggaagg gcgaggccct ggagcaggcc atcatcagcc
121aggccccgca ggtggagaag ctcattgcta cgacggccca cgagcggatg ccctggtacc
181acagcagcct gacgcgtgag gaggccgagc gcaaacttta ctctggggcg cagaccgacg
241gcaagttcct gctgaggccg cggaaggagc agggcacata cgccctgtcc ctcatctatg
301ggaagacggt gtaccactac ctcatcagcc aagacaaggc gggcaagtac tgcattcccg
361agggcaccaa gtttgacacg ctctggcagc tggtggagta tctgaagctg aaggcggacg
421ggctcatcta ctgcctgaag gaggcctgcc ccaacagcag tgccagcaac gcctcagggg
481ctgctgctcc cacactccca gcccacccat ccacgttgac tcatcctcag agacgaatcg
541acaccctcaa ctcagatgga tacacccctg agccagcacg cataacgtcc ccagacaaac
601cgcggccgat gcccatggac acgagcgtgt atgagagccc ctacagcgac ccagaggagc
661tcaaggacaa gaagctcttc ctgaagcgcg ataacctcct catagctgac attgaacttg
721gctgcggcaa ctttggctca gtgcgccagg gcgtgtaccg catgcgcaag aagcagatcg
781acgtggccat caaggtgctg aagcagggca cggagaaggc agacacggaa gagatgatgc
841gcgaggcgca gatcatgcac cagctggaca acccctacat cgtgcggctc attggcgtct
901gccaggccga ggccctcatg ctggtcatgg agatggctgg gggcgggccg ctgcacaagt
961tcctggtcgg caagagggag gagatccctg tgagcaatgt ggccgagctg ctgcaccagg
1021tgtccatggg gatgaagtac ctggaggaga agaactttgt gcaccgtgac ctggcggccc
1081gcaacgtcct gctggttaac cggcactacg ccaagatcag cgactttggc ctctccaaag
1141cactgggtgc cgacgacagc tactacactg cccgctcagc agggaagtgg ccgctcaagt
1201ggtacgcacc cgaatgcatc aacttccgca agttctccag ccgcagcgat gtctggagct
1261atggggtcac catgtgggag gccttgtcct acggccagaa gccctacaag aagatgaaag
1321ggccggaggt catggccttc atcgagcagg gcaagcggat ggagtgccca ccagagtgtc
1381cacccgaact gtacgcactc atgagtgact gctggatcta caagtgggag gatcgccccg
1441acttcctgac cgtggagcag cgcatgcgag cctgttacta cagcctggcc agcaaggtgg
1501aagggccccc aggcagcaca cagaaggctg aggctgcctg tgcctnncca actttcttgt
1561acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1621gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.