Construct: ORF ccsbBroad304_15622

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014066.1_s304c1, BRDN0000407908
Derived from:
ccsbBroadEn_15622
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ZKSCAN1 (7586)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15622
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7586 ZKSCAN1 zinc finger with KRAB and S... NM_001346581.2 99.9% 99.8% 77T>C
2 human 7586 ZKSCAN1 zinc finger with KRAB and S... NM_003439.4 99.9% 99.8% 77T>C
3 human 7586 ZKSCAN1 zinc finger with KRAB and S... XM_011516559.3 99.9% 99.8% 77T>C
4 human 7586 ZKSCAN1 zinc finger with KRAB and S... NM_001287054.3 93.6% 93.6% 0_1ins108
5 human 7586 ZKSCAN1 zinc finger with KRAB and S... NM_001346580.2 93.6% 93.6% 0_1ins108
6 human 7586 ZKSCAN1 zinc finger with KRAB and S... NM_001287055.2 62.1% 62.1% 0_1ins639
7 human 7586 ZKSCAN1 zinc finger with KRAB and S... NM_001346579.1 51.5% 43.7% (many diffs)
8 human 7586 ZKSCAN1 zinc finger with KRAB and S... NR_144477.2 17.4% (many diffs)
9 human 7586 ZKSCAN1 zinc finger with KRAB and S... XR_927529.2 16.6% (many diffs)
10 human 7586 ZKSCAN1 zinc finger with KRAB and S... XR_002956485.1 16.1% (many diffs)
11 mouse 74570 Zkscan1 zinc finger with KRAB and S... NM_133906.4 85.4% 89.5% (many diffs)
12 mouse 74570 Zkscan1 zinc finger with KRAB and S... NM_029869.1 74.2% 78.3% (many diffs)
13 mouse 74570 Zkscan1 zinc finger with KRAB and S... XM_006504628.3 74.2% 78.3% (many diffs)
14 mouse 74570 Zkscan1 zinc finger with KRAB and S... XM_011240958.2 53.1% 54.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1755
ORF length:
1689
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat gactgctgaa tcacgggaag ccacgggtct gtccccacag gctgcacagg
121agaaggatgg tatcgtaata gcgaaggtgg aagaggaaga tgaggaagac cacatgtggg
181ggcaggattc caccctacag gacacgcctc ctccagaccc agagatattc cgccaacgct
241tcaggcgctt ctgttaccag aacacttttg ggccccgaga ggctctcagt cggctgaagg
301aactttgtca tcagtggctg cggccagaaa taaacaccaa ggaacagatc ctggagcttc
361tggtgctaga gcagtttctt tccatcctgc ccaaggagct ccaggtctgg ctgcaggaat
421accgccccga tagtggagag gaggccgtga cccttctaga agacttggag cttgatttat
481caggacaaca ggtcccaggt caagttcatg gacctgagat gctcgcaagg gggatggtgc
541ctctggatcc agttcaggag tcctcgagct ttgaccttca tcacgaggcc acccagtccc
601acttcaaaca ttcgtctcgg aaaccccgcc tcttacagtc acgagctctt cctgctgccc
661acattcctgc accccctcat gagggtagtc ccagagacca ggcgatggca tctgcactat
721tcacagcgga ttcccaggca atggtgaaga tcgaggacat ggctgtgtcc ctcattctgg
781aggaatgggg atgtcagaat ctggctcgga ggaatctcag tagggacaac aggcaggaga
841attatgggag cgcatttccc cagggtggtg aaaacaggaa tgagaacgag gagtcaacct
901caaaggctga aacctcggaa gattcagcat cacgcgggga gacaacagga agatcccaga
961aagagtttgg agagaaacgt gaccaggagg gcaaaacagg agaaagacag cagaaaaacc
1021ctgaggagaa aaccaggaaa gagaaaagag attcagggcc agctatagga aaggacaaaa
1081aaaccatcac aggagagaga ggtccaaggg agaaggggaa aggattggga agaagcttca
1141gtctgagctc caacttcacc acccctgaag aagttcccac gggaacaaag tctcacagat
1201gtgatgaatg tggtaaatgc ttcacgagaa gttcaagcct tatccgccat aaaataatcc
1261acactggaga aaagccctat gaatgtagtg agtgtgggaa agccttcagt cttaactcca
1321accttgtcct gcatcagagg atccacacag gagagaaacc tcatgaatgt aacgagtgtg
1381gcaaggcctt cagccacagt tccaatctca tcctccatca gcgcatccac tctggagaga
1441aaccttatga atgtaatgag tgcgggaagg ccttcagcca gagctcggac ctcaccaagc
1501atcagagaat tcacacgggg gagaaaccct atgaatgtag tgaatgtgga aaagctttca
1561accgaaactc atacctgatt ttgcatcgga gaattcacac tcgagaaaag ccctacaagt
1621gcactaagtg tggcaaggcc ttcacccgca gctccaccct cactctgcat cacagaatcc
1681atgccagaga gagagcctct gagtacagcc cagcctccct tgatgcattt ggcgcgttcc
1741tgaaaagttg tgtgtnncca actttcttgt acaaagtggt tggtaagcct atccctaacc
1801ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1861cgacaatcaa cctctggatt a

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