Construct: ORF ccsbBroad304_15657
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013519.1_s304c1, BRDN0000408082
- Derived from:
- ccsbBroadEn_15657
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGEF1 (9138)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15657
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9138 | ARHGEF1 | Rho guanine nucleotide exch... | NM_198977.2 | 100% | 100% | |
| 2 | human | 9138 | ARHGEF1 | Rho guanine nucleotide exch... | NM_004706.4 | 96.3% | 96.3% | 225_323del |
| 3 | human | 9138 | ARHGEF1 | Rho guanine nucleotide exch... | NM_199002.2 | 94.8% | 94.8% | 1_45del;270_368del |
| 4 | mouse | 16801 | Arhgef1 | Rho guanine nucleotide exch... | NM_001130152.1 | 82.1% | 84.1% | (many diffs) |
| 5 | mouse | 16801 | Arhgef1 | Rho guanine nucleotide exch... | NM_001130153.1 | 82.1% | 84.1% | (many diffs) |
| 6 | mouse | 16801 | Arhgef1 | Rho guanine nucleotide exch... | NM_008488.2 | 82% | 84% | (many diffs) |
| 7 | mouse | 16801 | Arhgef1 | Rho guanine nucleotide exch... | NM_001130151.1 | 77.6% | 79.2% | (many diffs) |
| 8 | mouse | 16801 | Arhgef1 | Rho guanine nucleotide exch... | XM_006539564.2 | 77.3% | 78.9% | (many diffs) |
| 9 | mouse | 16801 | Arhgef1 | Rho guanine nucleotide exch... | XM_006539565.3 | 77.3% | 78.9% | (many diffs) |
| 10 | mouse | 16801 | Arhgef1 | Rho guanine nucleotide exch... | XM_017322003.1 | 77.3% | 78.9% | (many diffs) |
| 11 | mouse | 16801 | Arhgef1 | Rho guanine nucleotide exch... | NM_001130150.1 | 77.3% | 78.8% | (many diffs) |
| 12 | mouse | 16801 | Arhgef1 | Rho guanine nucleotide exch... | XR_391047.2 | 64.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2703
- ORF length:
- 2637
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga agacttcgcc cgaggggcgg cctccccagg cccctcccgg cctggcctgg 121 ttcccgtcag catcatcggg gctgaggatg aggattttga gaacgagctg gagacaaact 181 cagaagagca aaacagccag ttccagagcc tggagcaggt gaagcggcgc ccagcccacc 241 tcatggccct cctgcagcac gtggccctgc agtttgagcc aggacccctg gttctccggg 301 tgccggtccc tcccaacgtc gcctttgaac ttgaccgcac tagggctgac ctcatctccg 361 aggatgtcca gcggcggttc gtgcaggagg tggtgcaaag ccagcaggta gccgtgggcc 421 ggcagctgga ggacttccgt tccaagcggc tcatgggcat gacgccctgg gagcaggagc 481 tggcccagct ggaggcttgg gttgggcggg accgagccag ctacgaggcc cgggagcggc 541 acgtggcgga gcggctgctc atgcacctgg aggagatgca acataccatc tctaccgacg 601 aagaaaagag tgctgccgtg gtcaacgcca ttggcctgta catgcgccac cttggggtgc 661 ggaccaagag tggagacaag aagtcgggga ggaacttctt ccggaaaaag gtgatgggga 721 accggcggtc ggacgagcct gccaagacca agaaggggct gagcagcatc ctggatgccg 781 cccgctggaa ccggggagag ccccaggttc cagattttcg acacctcaaa gcagaggttg 841 atgccgagaa gccaggtgct acagaccgga agggaggcgt ggggatgccc tctcgggacc 901 ggaatatcgg ggctcctggg caggacaccc ctggagtctc tctgcaccct ctgtccctgg 961 acagcccaga ccgggaacca ggtgctgacg cccccctgga gctgggggac tcatccccgc 1021 agggcccaat gagcctggag tccttggcgc ccccagagag taccgacgag ggggccgaaa 1081 ccgagagccc cgagcctgga gatgaggggg agccggggcg gtcgggactg gagcttgaac 1141 cagaagagcc tcccggctgg cgggaactcg tccccccaga caccctgcac agcctgccca 1201 agagccaggt gaagcggcag gaggtcatca gcgagctgct ggtgacagag gcggcccacg 1261 tgcgcatgct gcgggtgctg cacgacctct tcttccagcc catggcagaa tgcctgttct 1321 tccccttgga ggagctgcag aacatcttcc ccagcctgga cgagctcatc gaggtgcatt 1381 ccctgttcct cgatcgcctg atgaagcgga ggcaggagag tggctacctc atcgaggaga 1441 tcggagacgt gctgctggcc cggtttgatg gtgctgaggg ctcctggttc cagaaaatct 1501 cctcccgctt ctgcagccgc cagtcatttg ccttagagca gctcaaagcc aagcaacgca 1561 aggaccctcg gttctgtgcc ttcgtgcagg aagctgagag ccgcccgcgg tgccgccgcc 1621 tgcagctgaa ggacatgatc cccacggaga tgcagcggct gaccaagtac cccctgctcc 1681 tgcagagcat cgggcagaac acagaagagc ccacagaacg ggagaaagtg gagctggcag 1741 ccgagtgctg ccgggaaatt ctacaccacg tcaaccaagc cgtgcgtgac atggaggacc 1801 tgctgaggct caaggactat cagcggcgcc tggacttgtc ccaccttcgg cagagcagcg 1861 accctatgct gagcgagttc aagaacctgg acatcaccaa gaagaaattg gtccacgagg 1921 gcccactgac gtggcgggtg actaaggaca aggcagtgga ggtgcatgtg ctgctgctgg 1981 acgacctgct gctgctgctc cagcgccagg acgagcggct gctgctcaag tcccatagcc 2041 ggacactgac gcccacgccc gatggcaaga ccatgctgcg gcccgtgctg cggctcacct 2101 ccgccatgac ccgcgaggtg gccaccgatc acaaagcctt ctacgtcctt tttacctggg 2161 accaggaggc ccagatatac gagctggtgg cacagactgt gtcggagcgg aaaaactggt 2221 gtgctctcat cactgagact gccggatccc tgaaagtccc tgcccctgcc tctcgcccta 2281 agccccggcc cagcccgagc agcacccgag aacccctcct cagcagctct gagaacggca 2341 atggtggccg agagacgtct ccagctgatg cccggaccga gagaatcctc agtgacctcc 2401 tgcccttctg cagaccaggc cccgagggcc agctcgctgc cacggccctt cggaaagtgc 2461 tgtccctgaa gcagcttctg tttccggcgg aggaagacaa tggggcgggg cctcctcgag 2521 atggggatgg ggtcccaggg ggcggccccc tgagcccagc acggacccag gaaatccagg 2581 agaacctgct cagcttggag gagaccatga agcagctgga ggagttggag gaggaatttt 2641 gccgcctgag acccctcctg tctcagcttg gggggaactc tgtcccccag cctggctgca 2701 cttnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 2761 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 2821 tctggatta