Construct: ORF ccsbBroad304_15693
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001200.1_s304c1, BRDN0000405432
- Derived from:
- ccsbBroadEn_15693
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PREB (10113)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15693
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10113 | PREB | prolactin regulatory elemen... | NM_001330484.1 | 100% | 100% | |
| 2 | human | 10113 | PREB | prolactin regulatory elemen... | NM_013388.6 | 82.7% | 82.7% | 1_216del |
| 3 | human | 10113 | PREB | prolactin regulatory elemen... | NM_001330485.1 | 68.8% | 68.8% | 1_216del;749_750ins174 |
| 4 | human | 10113 | PREB | prolactin regulatory elemen... | NM_001330486.1 | 66.6% | 62.2% | 1_216del;999_1000ins160;1050_1051ins41 |
| 5 | human | 10113 | PREB | prolactin regulatory elemen... | XR_939649.3 | 58.1% | (many diffs) | |
| 6 | human | 10113 | PREB | prolactin regulatory elemen... | NM_001330487.1 | 52.7% | 48.4% | (many diffs) |
| 7 | human | 10113 | PREB | prolactin regulatory elemen... | NR_138479.1 | 46.2% | 1_470del;1179_1204del;1532_2238del | |
| 8 | mouse | 50907 | Preb | prolactin regulatory elemen... | NM_016703.3 | 71.5% | 72.9% | (many diffs) |
| 9 | mouse | 50907 | Preb | prolactin regulatory elemen... | NM_001294302.1 | 57.4% | 55.6% | (many diffs) |
| 10 | mouse | 50907 | Preb | prolactin regulatory elemen... | XM_006504015.2 | 52.4% | 50.3% | (many diffs) |
| 11 | mouse | 50907 | Preb | prolactin regulatory elemen... | XM_006504017.3 | 48.2% | 48.6% | (many diffs) |
| 12 | mouse | 50907 | Preb | prolactin regulatory elemen... | XR_376814.3 | 15.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1104
- ORF length:
- 1035
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacttggca ctggctggtg acatccttgc tgcagggcag gatgcccact 121 gtcagctcct gcgcttccag gcacatcaac agcagggcaa caaggcagag aaggccggtt 181 ccaaggagca ggggcctcga caaaggaagg gagcagcccc agcagagaag aaatgtggag 241 cggaaaccca gcacgagggg ctagaactca gggtagagaa tttgcaggcg gtgcagacag 301 actttagctc cgatccactg cagaaagttg tgtgcttcaa ccacgataat accctgcttg 361 ccactggagg aacagatggc tacgtccgtg tctggaaggt gcccagcctg gagaaggttc 421 tggagttcaa agcccacgaa ggggagattg aagacctggc tttagggcct gatggcaagt 481 tggtaaccgt gggccgggac cttaaggcct ctgtgtggca gaaggatcag ctggtgacac 541 agctgcactg gcaagaaaat ggacccacct tttccagcac accttaccgc taccaggcct 601 gcaggtttgg gcaggttcca gaccagcctg ctggcctgcg actcttcaca gtgcaaattc 661 cccacaagcg cctgcgccag ccccctccct gctacctcac agcctgggat ggctccaact 721 tcttgcccct tcggaccaag tcctgtggcc atgaagtcgt ctcctgcctc gatgtcagtg 781 aatccggcac cttcctaggc ctgggcacag tcactggctc tgttgccatc tacatagctt 841 tctctctcca gtgcctctac tacgtgaggg aggcccatgg cattgtggtg acggatgtgg 901 cctttctacc tgagaagggt cgtggtccag agctccttgg gtcccatgaa actgccctgt 961 tctctgtggc tgtggacagt cgttgccagc tgcatctgtt gccctcacgg cggagtgttc 1021 ctgtgtggct cctgctcctg ctgtgtgtcg ggcttattat tgtgaccatc ctgctgctcc 1081 agagtgcctt tccaggtttc ctttnnccaa ctttcttgta caaagtggtt ggtaagccta 1141 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1201 gcgttaagtc gacaatcaac ctctggatta