Construct: ORF ccsbBroad304_15831
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012903.1_s304c1, BRDN0000400045
- Derived from:
- ccsbBroadEn_15831
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUSAP1 (51203)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15831
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_018454.8 | 100% | 100% | |
| 2 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_016359.5 | 99.7% | 99.7% | 659_661delAGC |
| 3 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_001243142.2 | 99.7% | 99.7% | 303_304insCAG |
| 4 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_001301136.2 | 99.5% | 99.5% | 303_304insCAG;656_658delAGC |
| 5 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_005254428.3 | 98.6% | 98.6% | 448_465del |
| 6 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720559.3 | 98.4% | 98.4% | 448_465del;677_679delAGC |
| 7 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720560.4 | 98.2% | 98.2% | 303_304insCAG;445_462del;674_676delAGC |
| 8 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_005254430.5 | 96.5% | 96.5% | 303_304ins45 |
| 9 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_001243143.2 | 96.3% | 96.3% | 303_304ins45;614_616delAGC |
| 10 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720561.3 | 95% | 95% | 303_304ins45;403_420del;632_634delAGC |
| 11 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720563.3 | 91.1% | 91.1% | 1002_1003ins117 |
| 12 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_005254431.3 | 89.9% | 89.9% | 448_465del;1020_1021ins117 |
| 13 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720562.3 | 89.7% | 89.7% | 448_465del;677_679delAGC;1023_1024ins117 |
| 14 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_017022294.2 | 87.7% | 87.7% | 303_304ins45;957_958ins117 |
| 15 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_001243144.2 | 85.9% | 85.9% | 92_93ins69;933_934ins117 |
| 16 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_017022295.1 | 55.5% | 55.5% | 0_1ins585;74_76delAGC |
| 17 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XR_001751299.2 | 51.7% | (many diffs) | |
| 18 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XR_001751300.2 | 50% | (many diffs) | |
| 19 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XR_001751301.2 | 45.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat catcccctct ctagaggagc tggactccct caagtacagt gacctgcaga 121 acttagccaa gagtctgggt ctccgggcca acctgagggc aaccaagttg ttaaaagcct 181 tgaaaggcta cattaaacat gaggcaagaa aaggaaatga gaatcaggat gaaagtcaaa 241 cttctgcatc ctcttgtgat gagactgaga tacagatcag caaccaggaa gaagctgaga 301 gacagccact tggccatgtc accaaaacaa ggagaaggtg caagactgtc cgtgtggacc 361 ctgactcaca gcagaatcat tcagagataa aaataagtaa tcccactgaa ttccagaatc 421 atgaaaagca ggaaagccag gatctcagag ctactgcaaa agttccttct ccaccagacg 481 agcaccaaga agctgagaat gctgtttcct caggtaacag agattcaaag gtaccttcag 541 aaggaaagaa atctctctac acagatgagt catccaaacc tggaaaaaat aaaagaactg 601 caatcactac tccaaacttt aagaagcttc atgaagctca ttttaaggaa atggagtcca 661 ttgatcaata tattgagaga aaaaagaaac attttgaaga acacaattcc atgaatgaac 721 tgaagcagcc catcaataag ggaggggtca ggactccagt acctccaaga ggaagactct 781 ctgtggcttc tactcccatc agccaacgac gctcgcaagg ccggtcttgt ggccctgcaa 841 gtcagagtac cttgggtctg aaggggtcac tcaagcgctc tgctatctct gcagctaaaa 901 cgggtgtcag gttttcagct gctactaaag ataatgagca taagcgttca ctgaccaaga 961 ctccagccag aaagtctgca catgtgaccg tgtctggggg caccccaaaa ggcgaggctg 1021 tgcttgggac acacaaatta aagaccatca cggggaattc tgctgctgtt attaccccat 1081 tcaagttgac aactgaggca acgcagactc cagtctccaa taagaaacca gtgtttgatc 1141 ttaaagcaag tttgtctcgt cccctcaact atgaaccaca caaaggaaag ctaaaaccat 1201 gggggcaatc taaagaaaat aattatctaa atcaacatgt caacagaatt aacttctaca 1261 agaaaactta caaacaaccc catctccaga caaaggaaga gcaacggaag aaacgcgagc 1321 aagaacgaaa ggagaagaaa gcaaaggttt tgggaatgcg aaggggcctc attttggctg 1381 aagattnncc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1441 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1501 acctctggat ta