Construct: ORF ccsbBroad304_15861
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011479.1_s304c1, BRDN0000408391
- Derived from:
- ccsbBroadEn_15861
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BS1 (54145)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15861
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54145 | H2BS1 | H2B.S histone 1 | NM_017445.3 | 99.2% | 99.2% | 83G>A;153C>T;354C>T |
| 2 | human | 102724334 | LOC102724334 | histone H2B type F-S-like | XM_006723918.4 | 99.2% | 99.2% | 83G>A;153C>T;354C>T |
| 3 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_001312653.1 | 98.6% | 99.2% | (many diffs) |
| 4 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_080593.2 | 98.6% | 99.2% | (many diffs) |
| 5 | human | 8344 | H2BC6 | H2B clustered histone 6 | NM_003523.2 | 93.3% | 98.4% | (many diffs) |
| 6 | human | 8970 | H2BC11 | H2B clustered histone 11 | NM_021058.3 | 92.3% | 98.4% | (many diffs) |
| 7 | human | 8340 | H2BC13 | H2B clustered histone 13 | NM_003519.3 | 92% | 96.8% | (many diffs) |
| 8 | human | 8341 | H2BC15 | H2B clustered histone 15 | NM_003520.3 | 91.5% | 97.6% | (many diffs) |
| 9 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001024599.4 | 91.5% | 96.8% | (many diffs) |
| 10 | human | 8343 | H2BC7 | H2B clustered histone 7 | NM_003522.3 | 90.7% | 98.4% | (many diffs) |
| 11 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 90.7% | 96.8% | (many diffs) |
| 12 | human | 8345 | H2BC9 | H2B clustered histone 9 | NM_003524.2 | 90.4% | 97.6% | (many diffs) |
| 13 | human | 8349 | H2BC21 | H2B clustered histone 21 | NM_003528.2 | 90.4% | 97.6% | (many diffs) |
| 14 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_021063.3 | 90.4% | 97.6% | (many diffs) |
| 15 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_138720.2 | 90.4% | 97.6% | (many diffs) |
| 16 | human | 8346 | H2BC10 | H2B clustered histone 10 | NM_003525.2 | 90.2% | 98.4% | (many diffs) |
| 17 | human | 8347 | H2BC4 | H2B clustered histone 4 | NM_003526.2 | 89.9% | 98.4% | (many diffs) |
| 18 | human | 8339 | H2BC8 | H2B clustered histone 8 | NM_003518.3 | 87.8% | 98.4% | (many diffs) |
| 19 | human | 8342 | H2BC14 | H2B clustered histone 14 | NM_003521.2 | 87.5% | 95.2% | (many diffs) |
| 20 | human | 128312 | H2BU1 | H2B.U histone 1 | NM_175055.2 | 87.5% | 93.6% | (many diffs) |
| 21 | human | 3018 | H2BC3 | H2B clustered histone 3 | NM_021062.2 | 86.5% | 96% | (many diffs) |
| 22 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001161334.1 | 86% | 91% | (many diffs) |
| 23 | human | 3017 | H2BC5 | H2B clustered histone 5 | XM_005249039.4 | 76% | 82% | (many diffs) |
| 24 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160944.2 | 68.9% | (many diffs) | |
| 25 | human | 337873 | H2BC20P | H2B clustered histone 20, p... | NR_036461.1 | 58.1% | (many diffs) | |
| 26 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160915.1 | 21.4% | (many diffs) | |
| 27 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_027337.2 | 18.8% | (many diffs) | |
| 28 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 17.4% | (many diffs) | |
| 29 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160942.1 | 16.1% | (many diffs) | |
| 30 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 15.7% | (many diffs) | |
| 31 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160913.1 | 14% | (many diffs) | |
| 32 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160914.1 | 11.5% | (many diffs) | |
| 33 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_001290380.1 | 92% | 98.4% | (many diffs) |
| 34 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_023422.3 | 92% | 98.4% | (many diffs) |
| 35 | mouse | 319181 | Hist1h2bg | histone cluster 1, H2bg | NM_178196.4 | 92% | 98.4% | (many diffs) |
| 36 | mouse | 319180 | Hist1h2bf | histone cluster 1, H2bf | NM_178195.2 | 92% | 97.6% | (many diffs) |
| 37 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001177653.1 | 91.7% | 98.4% | (many diffs) |
| 38 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001290530.1 | 91.7% | 98.4% | (many diffs) |
| 39 | mouse | 319184 | Hist1h2bk | histone cluster 1, H2bk | NM_175665.2 | 91.7% | 98.4% | (many diffs) |
| 40 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_178194.4 | 91.7% | 98.4% | (many diffs) |
| 41 | mouse | 319186 | Hist1h2bm | histone cluster 1, H2bm | NM_178200.2 | 91.7% | 97.6% | (many diffs) |
| 42 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001313878.1 | 91.5% | 97.6% | (many diffs) |
| 43 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001313880.1 | 91.5% | 97.6% | (many diffs) |
| 44 | mouse | 319182 | Hist1h2bh | histone cluster 1, H2bh | NM_178197.2 | 91.5% | 97.6% | (many diffs) |
| 45 | mouse | 319185 | Hist1h2bl | histone cluster 1, H2bl | NM_178199.2 | 91.5% | 97.6% | (many diffs) |
| 46 | mouse | 319187 | Hist1h2bn | histone cluster 1, H2bn | NM_178201.2 | 91.2% | 97.6% | (many diffs) |
| 47 | mouse | 319183 | Hist1h2bj | histone cluster 1, H2bj | NM_178198.2 | 91% | 97.6% | (many diffs) |
| 48 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_001290466.1 | 90.4% | 96% | (many diffs) |
| 49 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | XM_006516690.2 | 90.4% | 96% | (many diffs) |
| 50 | mouse | 319178 | Hist1h2bb | histone cluster 1, H2bb | NM_175664.3 | 89.6% | 95.2% | (many diffs) |
| 51 | mouse | 319189 | Hist2h2bb | histone cluster 2, H2bb | NM_175666.2 | 88.8% | 96.8% | (many diffs) |
| 52 | mouse | 78303 | Hist3h2ba | histone cluster 3, H2ba | NM_030082.4 | 86.6% | 92% | (many diffs) |
| 53 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001097979.2 | 85.8% | 91% | (many diffs) |
| 54 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001110555.1 | 85.8% | 91% | (many diffs) |
| 55 | mouse | 319190 | Hist2h2be | histone cluster 2, H2be | NM_178214.4 | 85.4% | 96% | (many diffs) |
| 56 | mouse | 319177 | Hist1h2ba | histone cluster 1, H2ba | NM_175663.2 | 83.7% | 85.8% | (many diffs) |
| 57 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_178202.2 | 82.3% | 87.6% | (many diffs) |
| 58 | mouse | 382522 | Hist3h2bb-ps | histone cluster 3, H2bb, ps... | NR_134309.1 | 70.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 447
- ORF length:
- 378
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gccggagcca gcgaagtccg ctcccgcgcc caagaagggc tcgaagaaag 121 ccgtgactaa ggcgcagaag aaggacggca agaagcgcaa gcgcagccgc aaggagagct 181 actccgtata cgtgtacaag gtgctgaagc aggtccaccc tgacaccggc atctcctcta 241 aggccatggg aatcatgaac tccttcgtca acgacatctt cgaacgcatc gcaggtgagg 301 cttcccgcct gccgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg 361 ccgtgcgcct gctgctgccc ggggagttgg ccaagcacgc cgtgtccgag ggcaccaagg 421 ctgtcaccaa gtacaccagc gctaagtnnc caactttctt gtacaaagtg gttggtaagc 481 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 541 ggcgcgttaa gtcgacaatc aacctctgga tta