Construct: ORF ccsbBroad304_15900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017012.1_s304c1, BRDN0000401518
- Derived from:
- ccsbBroadEn_15900
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM36 (55521)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15900
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55521 | TRIM36 | tripartite motif containing 36 | NM_018700.4 | 99.9% | 99.7% | 1283A>G;2032G>A |
| 2 | human | 55521 | TRIM36 | tripartite motif containing 36 | NM_001300759.2 | 97.8% | 97.1% | (many diffs) |
| 3 | human | 55521 | TRIM36 | tripartite motif containing 36 | XM_017009621.2 | 97.6% | 97.4% | 298_348del;1334A>G;2083G>A |
| 4 | human | 55521 | TRIM36 | tripartite motif containing 36 | XM_017009622.2 | 95.6% | 94.8% | (many diffs) |
| 5 | human | 55521 | TRIM36 | tripartite motif containing 36 | NM_001300752.2 | 78.6% | 78.4% | 0_1ins465;818A>G;1567G>A |
| 6 | human | 55521 | TRIM36 | tripartite motif containing 36 | XR_002956164.1 | 73.9% | (many diffs) | |
| 7 | human | 55521 | TRIM36 | tripartite motif containing 36 | XM_017009623.2 | 68.6% | 68.4% | (many diffs) |
| 8 | mouse | 28105 | Trim36 | tripartite motif-containing 36 | NM_178872.4 | 86.5% | 92.3% | (many diffs) |
| 9 | mouse | 28105 | Trim36 | tripartite motif-containing 36 | NM_001170855.1 | 85.1% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2253
- ORF length:
- 2184
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcggagtct ggggagatga gtgaatttgg ctacatcatg gaattgatag 121 ctaaaggcaa ggttaccatt aagaatatcg aaagggagct catttgccca gcatgcaagg 181 agctgtttac ccacccattg attctccctt gccaacatag tatctgtcat aaatgtgtaa 241 aagaactcct gctgactctc gatgattcat tcaacgatgt gggatcagac aactccaatc 301 aaagcagtcc tcgacttcgg ctcccctccc ctagtatgga taaaattgac cgaattaaca 361 gaccaggctg gaagcgcaat tcattgaccc cgaggacaac tgttttccct tgccctggct 421 gtgagcatga tgtggatctt ggagaacgag gaatcaatgg tctgtttcga aacttcactt 481 tggaaactat tgtggaaaga tatcgtcaag cagctagggc agccacagcc attatgtgtg 541 acctttgtaa accaccacct caagaatcca caaaaagctg catggactgt agtgcaagtt 601 actgcaatga atgcttcaaa attcatcacc cttggggtac tataaaagct caacatgagt 661 atgttggtcc aactactaac ttcagaccca agattttaat gtgcccagaa catgaaacag 721 agagaataaa catgtactgt gaattatgta ggaggccagt ttgccatctg tgtaagttgg 781 gtggtaatca tgccaaccac cgtgtaacca ctatgagcag tgcctacaaa accttaaagg 841 aaaagctttc aaaggatatt gattacctta ttggtaagga aagccaggtg aagagtcaaa 901 tatctgaact aaacttgtta atgaaagaaa cagagtgtaa tggagagagg gctaaagaag 961 aagcaattac acattttgaa aagctctttg aagttctgga agagaggaaa tcatctgttt 1021 tgaaagcaat tgactcctct aagaaactaa gattagacaa atttcagact caaatggaag 1081 agtaccaggg acttctagag aacaatggac ttgtgggata tgctcaagaa gtgctaaagg 1141 agacagatca gtcttgcttt gtgcagacag caaagcagct ccacctcaga atacagaaag 1201 ccacagaatc tttgaagagc tttagacctg cagctcagac ttcttttgaa gactatgttg 1261 ttaatacctc taaacaaaca gaacttcttg gagaattatc ctttttctct agtggcatag 1321 acgtgccaga gatcaatgag gaacagagca gagtttataa caatgccttg ataaattggc 1381 accatccaga aaaggataaa gctgatagct atgttcttga atatcggaaa atcaatagag 1441 atgatgaaat gtcatggaat gagatagaag tgtgtggaac aagtaaaata attcaagact 1501 tggaaaacag tagtacctat gctttcagag taagagctta caagggttca atctgtagtc 1561 cttgcagcag agaattgatt cttcatactc ctccagctcc agttttcagc ttcctctttg 1621 atgaaaaatg tggctataat aatgaacacc tcctgctgaa cttgaagaga gaccgtgtag 1681 agagtagagc tggatttaat cttctgcttg ctgcagaacg catccaagtg ggttattaca 1741 caagcttaga ctacatcatt ggagatactg gcattacaaa aggaaaacac ttctgggcct 1801 tccgtgtgga accatattca tacctggtaa aagtgggagt tgcttctagc gataaactac 1861 aagaatggct ccgttctccc cgggatgcag ttagtccaag atatgagcaa gacagtgggc 1921 atgacagtgg aagtgaggat gcctgttttg attcttcaca accatttacc ttagttacta 1981 taggcatgca gaaatttttt atacccaagt cacctacttc ttctaatgaa cctgaaaata 2041 gagttctccc tatgccaaca agtattggga ttttccttga ctgtgataaa ggcaaagtaa 2101 atttctatga tatggatcag atgaaatgcc tttatgaacg ccaagtggac tgttcacata 2161 cactgtatcc agcatttgca ttaatgggca gtggaggaat tcagcttgaa gaacccatca 2221 cagcaaaata tctggaatac caagaggaca tgtnnccaac tttcttgtac aaagtggttg 2281 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2341 accggtggcg cgttaagtcg acaatcaacc tctggatta