Construct: ORF ccsbBroad304_15900

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017012.1_s304c1, BRDN0000401518
Derived from:
ccsbBroadEn_15900
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRIM36 (55521)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15900
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55521 TRIM36 tripartite motif containing 36 NM_018700.4 99.9% 99.7% 1283A>G;2032G>A
2 human 55521 TRIM36 tripartite motif containing 36 NM_001300759.2 97.8% 97.1% (many diffs)
3 human 55521 TRIM36 tripartite motif containing 36 XM_017009621.2 97.6% 97.4% 298_348del;1334A>G;2083G>A
4 human 55521 TRIM36 tripartite motif containing 36 XM_017009622.2 95.6% 94.8% (many diffs)
5 human 55521 TRIM36 tripartite motif containing 36 NM_001300752.2 78.6% 78.4% 0_1ins465;818A>G;1567G>A
6 human 55521 TRIM36 tripartite motif containing 36 XR_002956164.1 73.9% (many diffs)
7 human 55521 TRIM36 tripartite motif containing 36 XM_017009623.2 68.6% 68.4% (many diffs)
8 mouse 28105 Trim36 tripartite motif-containing 36 NM_178872.4 86.5% 92.3% (many diffs)
9 mouse 28105 Trim36 tripartite motif-containing 36 NM_001170855.1 85.1% 90.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2253
ORF length:
2184
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtcggagtct ggggagatga gtgaatttgg ctacatcatg gaattgatag
121ctaaaggcaa ggttaccatt aagaatatcg aaagggagct catttgccca gcatgcaagg
181agctgtttac ccacccattg attctccctt gccaacatag tatctgtcat aaatgtgtaa
241aagaactcct gctgactctc gatgattcat tcaacgatgt gggatcagac aactccaatc
301aaagcagtcc tcgacttcgg ctcccctccc ctagtatgga taaaattgac cgaattaaca
361gaccaggctg gaagcgcaat tcattgaccc cgaggacaac tgttttccct tgccctggct
421gtgagcatga tgtggatctt ggagaacgag gaatcaatgg tctgtttcga aacttcactt
481tggaaactat tgtggaaaga tatcgtcaag cagctagggc agccacagcc attatgtgtg
541acctttgtaa accaccacct caagaatcca caaaaagctg catggactgt agtgcaagtt
601actgcaatga atgcttcaaa attcatcacc cttggggtac tataaaagct caacatgagt
661atgttggtcc aactactaac ttcagaccca agattttaat gtgcccagaa catgaaacag
721agagaataaa catgtactgt gaattatgta ggaggccagt ttgccatctg tgtaagttgg
781gtggtaatca tgccaaccac cgtgtaacca ctatgagcag tgcctacaaa accttaaagg
841aaaagctttc aaaggatatt gattacctta ttggtaagga aagccaggtg aagagtcaaa
901tatctgaact aaacttgtta atgaaagaaa cagagtgtaa tggagagagg gctaaagaag
961aagcaattac acattttgaa aagctctttg aagttctgga agagaggaaa tcatctgttt
1021tgaaagcaat tgactcctct aagaaactaa gattagacaa atttcagact caaatggaag
1081agtaccaggg acttctagag aacaatggac ttgtgggata tgctcaagaa gtgctaaagg
1141agacagatca gtcttgcttt gtgcagacag caaagcagct ccacctcaga atacagaaag
1201ccacagaatc tttgaagagc tttagacctg cagctcagac ttcttttgaa gactatgttg
1261ttaatacctc taaacaaaca gaacttcttg gagaattatc ctttttctct agtggcatag
1321acgtgccaga gatcaatgag gaacagagca gagtttataa caatgccttg ataaattggc
1381accatccaga aaaggataaa gctgatagct atgttcttga atatcggaaa atcaatagag
1441atgatgaaat gtcatggaat gagatagaag tgtgtggaac aagtaaaata attcaagact
1501tggaaaacag tagtacctat gctttcagag taagagctta caagggttca atctgtagtc
1561cttgcagcag agaattgatt cttcatactc ctccagctcc agttttcagc ttcctctttg
1621atgaaaaatg tggctataat aatgaacacc tcctgctgaa cttgaagaga gaccgtgtag
1681agagtagagc tggatttaat cttctgcttg ctgcagaacg catccaagtg ggttattaca
1741caagcttaga ctacatcatt ggagatactg gcattacaaa aggaaaacac ttctgggcct
1801tccgtgtgga accatattca tacctggtaa aagtgggagt tgcttctagc gataaactac
1861aagaatggct ccgttctccc cgggatgcag ttagtccaag atatgagcaa gacagtgggc
1921atgacagtgg aagtgaggat gcctgttttg attcttcaca accatttacc ttagttacta
1981taggcatgca gaaatttttt atacccaagt cacctacttc ttctaatgaa cctgaaaata
2041gagttctccc tatgccaaca agtattggga ttttccttga ctgtgataaa ggcaaagtaa
2101atttctatga tatggatcag atgaaatgcc tttatgaacg ccaagtggac tgttcacata
2161cactgtatcc agcatttgca ttaatgggca gtggaggaat tcagcttgaa gaacccatca
2221cagcaaaata tctggaatac caagaggaca tgtnnccaac tttcttgtac aaagtggttg
2281gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2341accggtggcg cgttaagtcg acaatcaacc tctggatta

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