Construct: ORF ccsbBroad304_15959

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000670.1_s304c1, BRDN0000412025
Derived from:
ccsbBroadEn_15959
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC22A23 (63027)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15959
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 63027 SLC22A23 solute carrier family 22 me... NM_001286455.1 99.9% 100% 678A>G
2 human 63027 SLC22A23 solute carrier family 22 me... NM_021945.6 99.9% 100% 678A>G
3 human 63027 SLC22A23 solute carrier family 22 me... XM_017011181.1 99.9% 100% 678A>G
4 human 63027 SLC22A23 solute carrier family 22 me... XM_017011186.1 99.9% 100% 678A>G
5 human 63027 SLC22A23 solute carrier family 22 me... XM_017011182.1 95.1% 94.3% (many diffs)
6 human 63027 SLC22A23 solute carrier family 22 me... XM_017011184.1 91.5% 91.6% 0_1ins102;576A>G
7 human 63027 SLC22A23 solute carrier family 22 me... XM_017011180.1 79% 79.1% 1_321del;999A>G
8 human 63027 SLC22A23 solute carrier family 22 me... NM_015482.1 58.9% 59% 1_843del;1521A>G
9 human 63027 SLC22A23 solute carrier family 22 me... XM_011514801.2 56.9% 57% 1_915del;1593A>G
10 human 63027 SLC22A23 solute carrier family 22 me... NR_104448.1 22.4% (many diffs)
11 mouse 73102 Slc22a23 solute carrier family 22, m... XM_006516794.3 62.7% 67.2% (many diffs)
12 mouse 73102 Slc22a23 solute carrier family 22, m... XM_006516793.3 52.9% 56.6% (many diffs)
13 mouse 73102 Slc22a23 solute carrier family 22, m... NM_001033167.3 52.2% 56% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1284
ORF length:
1215
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gttcagcaca ctcaggttct ttgaaggatt ttgcctggct ggaatcattc
121tcaccttgta tgctttacga atagagctgt gcccccctgg aaaacggttc atgattacga
181tggtggcgag cttcgtggcc atggcgggcc agttcctcat gcctgggcta gccgccctgt
241gccgggattg gcaggtgctg caggccctca tcatctgccc cttcctgctc atgctgctct
301actggtcgat attccccgag tccctccggt ggctaatggc cacccagcag tttgagtctg
361caaagaggct gatcctccac ttcacacaga agaatcgcat gaaccctgag ggcgacatca
421agggtgtgat accagagctg gagaaagagc tttcccggag gcccaagaag gtctgcatcg
481tgaaggtggt ggggacacgg aacctgtgga agaacattgt ggtcctgtgt gtgaactcgc
541tgacggggta cgggatccac cactgctttg ccaggagcat gatgggccac gaggtgaagg
601tgccgctcct ggagaacttc tatgctgact actataccac ggccagcatc gcgctggtgt
661cctgcctggc catgtgcgtg gtggtccgat tcctcgggcg caggggaggg ctgctgctct
721tcatgatcct caccgccctg gcctcgctcc tgcagctcgg cctcctcaac ctgattggaa
781agtacagcca gcacccagac tcagggatga gtgacagcgt caaggacaaa ttttccatcg
841cgttttccat cgtgggcatg tttgcctccc atgcggtggg gagcctcagc gtgttcttct
901gtgcggagat caccccgacg gtgataaggt gtggcgggct ggggctggtg ctggccagcg
961cgggcttcgg catgctgacg gcacccatca tcgagctgca caaccagaaa ggctacttcc
1021tgcaccacat catctttgcc tgctgcacgc tcatctgcat catctgcatc ctcctgctgc
1081ccgagagcag ggaccagaac ctgcctgaga acatttctaa cggggagcac tacacgcgcc
1141agccgctgct gccgcacaag aagggggagc agccactgct gctcaccaac gccgagctca
1201aggactactc gggcctccac gatgccgcag ccgcgggtga cacactgccc gagggtgcca
1261cggccaacgg catgaaggcc atgtnnccaa ctttcttgta caaagtggtt ggtaagccta
1321tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1381gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)