Construct: ORF ccsbBroad304_15964
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000615.1_s304c1, BRDN0000402164
- Derived from:
- ccsbBroadEn_15964
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TINAGL1 (64129)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15964
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | NM_001204415.1 | 60.2% | 60.2% | 1_432del |
| 2 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | XM_005271107.2 | 60.2% | 60.2% | 1_432del |
| 3 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | XM_011541946.1 | 53.4% | 53.4% | 1_570del |
| 4 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | NM_001204414.1 | 50% | 50% | 1_654del |
| 5 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | NM_022164.3 | 46.6% | 46.6% | 1_747del |
| 6 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | XM_005271106.3 | 36.7% | 26.2% | 1_747del;1092_1093ins124;1278_1320del |
| 7 | mouse | 94242 | Tinagl1 | tubulointerstitial nephriti... | NM_001168333.1 | 39.6% | 41.4% | (many diffs) |
| 8 | mouse | 94242 | Tinagl1 | tubulointerstitial nephriti... | NM_023476.3 | 39.6% | 41.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac gcctgtcctg tcgccccaga acctgctgtc ttgtgacacc caccagcagc 121 agggctgccg cggtgggcgt ctcgatggtg cctggtggtt cctgcgtcgc cgaggggtgg 181 tgtctgacca ctgctacccc ttctcgggcc gtgaacgaga cgaggctggc cctgcgcccc 241 cctgtatgat gcacagccga gccatgggtc ggggcaagcg ccaggccact gcccactgcc 301 ccaacagcta tgttaataac aatgacatct accaggtcac tcctgtctac cgcctcggct 361 ccaacgacaa ggagatcatg aaggagctga tggagaatgg ccctgtccaa gccctcatgg 421 aggtgcatga ggacttcttc ctatacaagg gaggcatcta cagccacacg ccagtgagcc 481 ttgggaggcc agagagatac cgccggcatg ggacccactc agtcaagatc acaggatggg 541 gagaggagac gctgccagat ggaaggacgc tcaaatactg gactgcggcc aactcctggg 601 gcccagcctg gggcgagagg ggccacttcc gcatcgtgcg cggcgtcaat gagtgcgaca 661 tcgagagctt cgtgctgggc gtctggggcc gcgtgggcat ggaggacatg ggtcatcact 721 nnccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 781 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 841 ggatta