Construct: ORF ccsbBroad304_15979
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002863.1_s304c1, BRDN0000407956
- Derived from:
- ccsbBroadEn_15979
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FTO (79068)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15979
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363905.1 | 15.9% | 15.8% | 1_839del;850_851insGGGCAGAGAGGAGTGTT |
| 2 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363900.1 | 11.7% | 11.5% | 1_1208del;1219_1220insGGGCAGAGAGGAGTGTT |
| 3 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363901.1 | 11.7% | 11.5% | 1_1208del;1219_1220insGGGCAGAGAGGAGTGTT |
| 4 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363898.1 | 11.4% | 11.3% | 1_1238del;1249_1250insGGGCAGAGAGGAGTGTT |
| 5 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363899.1 | 11.4% | 11.3% | 1_1238del;1249_1250insGGGCAGAGAGGAGTGTT |
| 6 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363896.1 | 11.4% | 10.6% | (many diffs) |
| 7 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363897.1 | 11.2% | 11% | 1_1274del;1285_1286insGGGCAGAGAGGAGTGTT |
| 8 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001080432.3 | 10.6% | 10.4% | 1_1352del;1363_1364insGGGCAGAGAGGAGTGTT |
| 9 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363891.1 | 10.4% | 10.2% | 1_1382del;1393_1394insGGGCAGAGAGGAGTGTT |
| 10 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NM_001363894.1 | 10.2% | 10% | 1_1415del;1426_1427insGGGCAGAGAGGAGTGTT |
| 11 | human | 79068 | FTO | FTO alpha-ketoglutarate dep... | NR_156761.1 | 4.8% | 1_602del;613_614insGGGCAGAGAGGAGTGTT;766_3320del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 246
- ORF length:
- 180
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc atgccagggc agagaggagt gttggtgcca gtcacgaatt gcccgaacat 121 tacctgctga tcagaagcca gaatgtcggc catactggga aaaggatgat gcttcgatgc 181 ctctgccgtt tgacctcaca gacatcgttt cagaactcag aggtcagctt ctggaagcaa 241 aaccctnncc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 301 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 361 acctctggat ta