Construct: ORF ccsbBroad304_16023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014983.1_s304c1, BRDN0000408094
- Derived from:
- ccsbBroadEn_16023
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YPEL3 (83719)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16023
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83719 | YPEL3 | yippee like 3 | NM_001145524.2 | 100% | 100% | |
| 2 | human | 29799 | YPEL1 | yippee like 1 | NM_013313.5 | 80.2% | 88.2% | (many diffs) |
| 3 | human | 83719 | YPEL3 | yippee like 3 | NM_031477.5 | 75.7% | 75.7% | 1_114del |
| 4 | human | 83719 | YPEL3 | yippee like 3 | XR_950857.1 | 32.5% | 1_249del;520_610del;698_1096del | |
| 5 | mouse | 66090 | Ypel3 | yippee-like 3 (Drosophila) | NM_025347.2 | 95.5% | 100% | (many diffs) |
| 6 | mouse | 66090 | Ypel3 | yippee-like 3 (Drosophila) | NM_026875.2 | 95.5% | 100% | (many diffs) |
| 7 | mouse | 77864 | Ypel2 | yippee-like 2 (Drosophila) | NM_001005341.3 | 79.9% | 89% | (many diffs) |
| 8 | mouse | 66090 | Ypel3 | yippee-like 3 (Drosophila) | XM_017312227.1 | 76.8% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 423
- ORF length:
- 357
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gcggatttca aagcccaaga cgtttcaggc ctacttggat gattgtcacc 121 ggaggtatag ctgtgcccac tgccgcgctc acctggccaa ccacgacgac ctcatctcca 181 agtccttcca gggcagtcag gggcgtgcct acctcttcaa ctcagtggtg aacgtgggct 241 gcgggccagc cgaggagcgg gtgctgctga ccggcctcca tgctgtcgcc gacatccact 301 gcgagaactg caagaccact ttgggctgga aatatgaaca ggcctttgag agcagccaga 361 agtacaaaga ggggaagtac atcattgaac tcaaccacat gatcaaagac aacggctggg 421 actnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 481 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 541 tctggatta