Construct: ORF ccsbBroad304_16027

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016991.1_s304c1, BRDN0000399950
Derived from:
ccsbBroadEn_16027
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACAD11 (84129)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_16027
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84129 ACAD11 acyl-CoA dehydrogenase fami... NM_032169.5 50.1% 50.1% 1_1167del
2 human 84129 ACAD11 acyl-CoA dehydrogenase fami... NR_132428.1 32.7% (many diffs)
3 human 84129 ACAD11 acyl-CoA dehydrogenase fami... NR_132426.1 28% 1_1547del;2067_2676del;3331_4180del
4 human 84129 ACAD11 acyl-CoA dehydrogenase fami... NR_132427.1 24.3% (many diffs)
5 human 100532724 NPHP3-ACAD11 NPHP3-ACAD11 readthrough (N... NR_037804.1 15% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1239
ORF length:
1173
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa acaacacatt cttccagctg aaaaggaggt aactgagttc tatgttcaaa
121atgaaaattc agtggacaag tggggaaaac ctttagtgat tgataaactc aaggaaatgg
181ccaaagtcga gggtctctgg aacttgtttt tgccagctgt cagcggactc agccacgtgg
241actatgcctt gattgctgaa gaaacaggaa aatgcttttt tgctccagat gtctttaact
301gccaagcacc agacacaggg aatatggagg ttctgcacct gtatggaagt gaggaacaga
361agaaacagtg gcttgagcct cttcttcaag ggaacattac ctcttgcttc tgtatgacag
421aacctgatgt agcttcaagt gatgccacga atattgaatg cagcatccaa cgagatgaag
481atagctatgt aattaacggc aaaaaatggt ggagcagtgg agctgggaat cccaagtgca
541aaattgcaat tgttttggga agaactcaaa atacttctct ctccagacac aaacagcaca
601gcatgattct tgttcccatg aacacacctg gagtaaaaat aataaggcct ttgtcagttt
661ttggctacac agataatttt catggaggac attttgagat ccattttaat caagtgcgag
721ttcctgccac aaatctaata ctaggtgaag gtaggggatt tgaaatttcc caaggccgcc
781ttggacctgg cagaatccac cactgtatga gaacagtagg tttggcggaa cgcgctttgc
841agatcatgtg tgagcgggca acacaaagga tagctttcaa gaagaagttg tatgcacatg
901aggttgtggc tcactggatt gctgaaagcc gcattgccat tgagaagatc cgcttgttga
961ctctgaaagc tgctcacagc atggacactc tgggcagtgc tggcgctaag aaagagattg
1021caatgatcaa agtggctgcc ccacgggctg tcagcaaaat cgttgactgg gccatccagg
1081tgtgcggagg tgctggtgtt tcccaggatt accctctggc taacatgtat gctataaccc
1141gagttttgcg tttagcagat ggacctgacg aagttcatct ttcagcaatc gcaacaatgg
1201agctgcggga ccaagccaaa agactgacag ccaagatatn nccaactttc ttgtacaaag
1261tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1321gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)