Construct: ORF ccsbBroad304_16047
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009263.1_s304c1, BRDN0000409310
- Derived from:
- ccsbBroadEn_16047
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBH1 (84893)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16047
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84893 | FBH1 | F-box DNA helicase 1 | NM_001258453.1 | 80.4% | 80.4% | 1_567del |
| 2 | human | 84893 | FBH1 | F-box DNA helicase 1 | XM_024448241.1 | 80.4% | 80.4% | 1_567del |
| 3 | human | 84893 | FBH1 | F-box DNA helicase 1 | NM_001258452.1 | 79.1% | 79.1% | 1_567del;2372_2422del |
| 4 | human | 84893 | FBH1 | F-box DNA helicase 1 | XM_024448240.1 | 79.1% | 79.1% | 1_567del;2372_2422del |
| 5 | human | 84893 | FBH1 | F-box DNA helicase 1 | XM_017016846.2 | 75.7% | 75.7% | 0_1ins567 |
| 6 | human | 84893 | FBH1 | F-box DNA helicase 1 | NM_178150.2 | 74.7% | 74.7% | 1_789del |
| 7 | human | 84893 | FBH1 | F-box DNA helicase 1 | XM_017016845.1 | 73.6% | 73.6% | 1_786del;2591_2641del |
| 8 | human | 84893 | FBH1 | F-box DNA helicase 1 | XM_017016844.2 | 73.5% | 73.5% | 1_789del;2594_2644del |
| 9 | human | 84893 | FBH1 | F-box DNA helicase 1 | NM_032807.4 | 71.2% | 71.2% | 1_942del |
| 10 | human | 84893 | FBH1 | F-box DNA helicase 1 | XM_011519748.2 | 71.2% | 71.2% | 1_942del |
| 11 | human | 84893 | FBH1 | F-box DNA helicase 1 | XM_017016842.1 | 70.2% | 70.2% | 1_942del;2747_2797del |
| 12 | human | 84893 | FBH1 | F-box DNA helicase 1 | XM_017016843.1 | 70.2% | 70.2% | 1_942del;2747_2797del |
| 13 | human | 84893 | FBH1 | F-box DNA helicase 1 | XR_002957028.1 | 66.9% | 1_727del;2260_2261ins76;2992_3307del | |
| 14 | human | 84893 | FBH1 | F-box DNA helicase 1 | XR_002957026.1 | 64.8% | (many diffs) | |
| 15 | human | 84893 | FBH1 | F-box DNA helicase 1 | XR_001747243.2 | 63% | 1_932del;2465_2466ins76;3197_3512del | |
| 16 | human | 84893 | FBH1 | F-box DNA helicase 1 | XR_001747244.2 | 62.4% | 1_932del;2243_2244ins100;3173_3488del | |
| 17 | human | 84893 | FBH1 | F-box DNA helicase 1 | XR_001747242.2 | 58.9% | (many diffs) | |
| 18 | mouse | 50755 | Fbxo18 | F-box protein 18 | NM_001348234.1 | 71.9% | 76.6% | (many diffs) |
| 19 | mouse | 50755 | Fbxo18 | F-box protein 18 | NM_001348235.1 | 71.9% | 76.6% | (many diffs) |
| 20 | mouse | 50755 | Fbxo18 | F-box protein 18 | NM_001348236.1 | 71.9% | 76.6% | (many diffs) |
| 21 | mouse | 50755 | Fbxo18 | F-box protein 18 | NM_001348237.1 | 71.9% | 76.6% | (many diffs) |
| 22 | mouse | 50755 | Fbxo18 | F-box protein 18 | NM_015792.2 | 66.7% | 71.2% | (many diffs) |
| 23 | mouse | 50755 | Fbxo18 | F-box protein 18 | XM_006497505.3 | 60.9% | 61.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2406
- ORF length:
- 2340
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgaagagcaa gctgtcagca aagtggacgg catcctgtct aactgtggca 121 tagaaaagga gtcagacctg tgtgtgctga acctcatacg atacacagcc accactaagt 181 gctctccgag tgtggatccc gagagggtgc tgtggagtct gagggaccac cccctcctcc 241 ccgaggctga ggcgtgtgtg cggcaacacc tccccgacct ctacgctgct gccgggggtg 301 tcaacatctg ggccctggtg gcggctgtgg tgctcctctc cagcagtgtg aatgacatcc 361 agcgactgct cttctgcctc cggagaccca gctccacggt gaccatgcca gatgtcaccg 421 agaccctgta ctgcatagcc gtgcttctct acgccatgag ggagaagggg attaacatca 481 gcaataggat tcactacaac attttctatt gcctatatct tcaggagaat tcctgcactc 541 aggccacaaa agttaaagag gagccatctg tctggccagg caagaaaacc atccaactta 601 cacatgaaca acagctgatt ctgaatcaca agatggaacc tctccaggtg gtgaaaatta 661 tggcctttgc cggcactggg aagacctcaa cgctggtcaa gtatgcagag aagtggtctc 721 agagcaggtt tctgtatgtg acattcaaca agagcatcgc aaagcaggcc gaacgcgtct 781 tccccagcaa cgtcatctgc aaaaccttcc actccatggc ctacgggcac atagggcgga 841 agtaccagtc aaagaagaag ttgaatctct tcaagttaac acccttcatg gtcaactccg 901 tccttgctga agggaagggt ggattcataa gagccaagct tgtgtgtaag actctagaaa 961 acttctttgc ctcggctgac gaagagctga ccattgatca cgtgcctatt tggtgtaaga 1021 acagccaagg acagagagtc atggttgagc agagtgaaaa actgaatggt gtccttgaag 1081 cgagccgcct ctgggataac atgcggaagc tgggggagtg cacagaagag gcgcaccaga 1141 tgactcatga cggctacttg aaactctggc agctgagcaa gccttcgctg gcctcttttg 1201 acgccatctt tgtggatgag gcccaggact gcacaccagc tatcatgaac atagttctgt 1261 ctcagccatg tgggaaaatc tttgtagggg acccgcacca gcagatctat accttccggg 1321 gtgcggtcaa cgccctgttc acagtgcccc acacccacgt cttctatctc acgcagagtt 1381 ttcggtttgg tgtggaaata gcttatgtgg gagctactat cttggatgtt tgcaagagag 1441 tcaggaaaaa gactttggtt ggaggaaacc atcagagtgg cattagaggt gacgcaaagg 1501 ggcaagtggc cttgttgtcc cggaccaacg ccaacgtgtt tgatgaggcc gtacgggtga 1561 cggaagggga attcccttca aggatacatt tgattggggg gattaaatca tttggattgg 1621 acagaatcat tgatatttgg atccttcttc agccagagga agaacggagg aaacaaaacc 1681 tcgtcattaa agacaaattt atcagaagat gggtgcacaa agaaggcttt agtggcttca 1741 agaggtatgt gaccgctgcc gaggacaagg agcttgaagc caagatcgca gttgttgaaa 1801 agtataacat caggattcca gagctggtgc aaaggataga aaaatgccat atagaagatt 1861 tggactttgc agagtacatt ctgggcactg tgcacaaagc caaaggcctg gagtttgaca 1921 ctgtgcatgt tttggatgat tttgtgaaag tgccttgtgc ccggcataac ctgccccagc 1981 ttccgcactt cagagttgag tcattttctg aggatgaatg gaatttactg tatgttgcag 2041 taactcgagc caagaagcgt ctcatcatga ccaaatcatt ggaaaacatt ttgactttgg 2101 ctggggagta cttcttgcaa gcagagctga caagcaacgt cttaaaaaca ggcgtggtgc 2161 gctgctgcgt gggacagtgc aacaatgcca tccctgttga caccgtcctt accatgaaga 2221 agctgcccat cacctatagc aacaggaagg aaaacaaggg gggctacctc tgccactcct 2281 gtgcggagca gcgcatcggg cccctggcgt tcctgacagc ctccccggag caggtgcgcg 2341 ccatggagcg cactgtggag aacatcgtac tgccccggca tgaggccctg ctcttcctcg 2401 tcttctnncc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2461 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2521 acctctggat ta