Construct: ORF ccsbBroad304_16069
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014092.1_s304c1, BRDN0000412044
- Derived from:
- ccsbBroadEn_16069
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM44 (93109)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16069
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93109 | TMEM44 | transmembrane protein 44 | NM_001166306.2 | 99.7% | 99.4% | 70C>A;198G>A;710A>G |
| 2 | human | 93109 | TMEM44 | transmembrane protein 44 | NM_001011655.3 | 92.2% | 77.1% | (many diffs) |
| 3 | human | 93109 | TMEM44 | transmembrane protein 44 | NM_138399.5 | 90.1% | 75.4% | (many diffs) |
| 4 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_005269371.4 | 89.9% | 75.3% | (many diffs) |
| 5 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513322.2 | 86.2% | 72.3% | (many diffs) |
| 6 | human | 93109 | TMEM44 | transmembrane protein 44 | NM_001166305.2 | 83.1% | 69.9% | (many diffs) |
| 7 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_017007518.1 | 83% | 64.1% | (many diffs) |
| 8 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_017007517.1 | 81% | 62.7% | (many diffs) |
| 9 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513320.2 | 78.2% | 65.9% | (many diffs) |
| 10 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513321.2 | 77.8% | 60.4% | (many diffs) |
| 11 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513319.2 | 76.7% | 59.6% | (many diffs) |
| 12 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513318.2 | 75.1% | 58.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1257
- ORF length:
- 1188
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggaggcg cccagccccg cgcccgcgct ctgggactgg gactacctgg 121 accgctgctt cgcccgcaac cgcgtctgca tctccttcgg cctgtggatc tgcgcctcct 181 cctgctggat cgccgcccac gcgctgcttc tctatctgag atgtgcacag aaacccagac 241 aggaccagtc ggcactgtgt gctgcatgct gcctcctgac cagtctgtgt gacaccgtcg 301 gggctcttct ggccagacag ctcacaatcc aggttttcac tggtgcctac ctagcagcta 361 ttgacttagt gaactttatg ttcattctct tcccagtctg tggatccaaa ttcaagtcta 421 attcagatcg ggaagcccga gagaggaaga ggaggcggca gctcagggcc agtgtgtttg 481 ccctggccct gccgctgagc ctgggcccgt gctgggctct gtgggttgct gtcccgaagg 541 cttcagccac catccggggg ccacagcgga ggctgctagc gagcctgctg caggaaaata 601 ctgagatcct cggctacctg ctgggtagcg ttgctgcctt tggctcctgg gcttctcgga 661 tcccccctct ctccagaatt tgccggggga agacatttcc ctccatccac ctgtggaccc 721 ggctcctgtc ggccctggct ggcctcctct atgcctcggc cattgtggcc cacgaccggc 781 accctgagta cctgctgcgg gccacaccct ggttcctgac ctccctcggc cgtgcggcac 841 tggacctcgc tattattttc ctttcgtgtg tgatgaagag caagatgaga caggccttag 901 gatttgccaa ggaagccaga gagagccctg acacccaagc ccttttgacc tgtgcagaga 961 aagaggaaga aaaccaggag aatttggatt gggtgcctct caccacactg tcacactgca 1021 agtcactgag gacaatgaca gcaatcagtc gctacatgga gctgaccatc gagcctgtgc 1081 agcaggctgc agtgccacca ggctgccagg tgacgggcag acgagcgccg gagatgcgtc 1141 cctgcaggac cccccgtcgt accctcccgt tcaggtcatc cgggcccggg tgtcttccgg 1201 cagctcctct gaggtctcct ccatcaactc cgacctggag tgggaccctg aagatgtnnc 1261 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1321 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1381 tta