Construct: ORF ccsbBroad304_16173
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019061.1_s304c1, BRDN0000464782
- Derived from:
- ccsbBroadEn_16173
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KRAS (3845)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16173
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3845 | KRAS | KRAS proto-oncogene, GTPase | NM_004985.3:c.35G>T | 100% | 100% | |
| 2 | human | 3845 | KRAS | KRAS proto-oncogene, GTPase | NM_001369787.1 | 99.8% | 99.4% | 35G>T |
| 3 | human | 3845 | KRAS | KRAS proto-oncogene, GTPase | NM_004985.5 | 99.8% | 99.4% | 35G>T |
| 4 | human | 3845 | KRAS | KRAS proto-oncogene, GTPase | NM_001369786.1 | 90.3% | 89.4% | (many diffs) |
| 5 | human | 3845 | KRAS | KRAS proto-oncogene, GTPase | NM_033360.4 | 90.3% | 89.4% | (many diffs) |
| 6 | mouse | 16653 | Kras | Kirsten rat sarcoma viral o... | NM_021284.6 | 93.4% | 96.8% | (many diffs) |
| 7 | mouse | 16653 | Kras | Kirsten rat sarcoma viral o... | XM_006506918.3 | 86.3% | 89.4% | (many diffs) |
| 8 | mouse | 16653 | Kras | Kirsten rat sarcoma viral o... | XM_006506919.3 | 77.3% | 80.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 633
- ORF length:
- 564
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gactgaatat aaacttgtgg tagttggagc tgttggcgta ggcaagagtg 121 ccttgacgat acagctaatt cagaatcatt ttgtggacga atatgatcca acaatagagg 181 attcctacag gaagcaagta gtaattgatg gagaaacctg tctcttggat attctcgaca 241 cagcaggtca agaggagtac agtgcaatga gggaccagta catgaggact ggggagggct 301 ttctttgtgt atttgccata aataatacta aatcatttga agatattcac cattatagag 361 aacaaattaa aagagttaag gactctgaag atgtacctat ggtcctagta ggaaataaat 421 gtgatttgcc ttctagaaca gtagacacaa aacaggctca ggacttagca agaagttatg 481 gaattccttt tattgaaaca tcagcaaaga caagacaggg tgttgatgat gccttctata 541 cattagttcg agaaattcga aaacataaag aaaagatgag caaagatggt aaaaagaaga 601 aaaagaagtc aaagacaaag tgtgtaatta tgtaaccaac tttcttgtac aaagtggttg 661 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 721 accggtggcg cgttaagtcg acaatcaacc tctggatta