Transcript: Mouse XM_006506919.3

PREDICTED: Mus musculus Kirsten rat sarcoma viral oncogene homolog (Kras), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Kras (16653)
Length:
4847
CDS:
255..938

Additional Resources:

NCBI RefSeq record:
XM_006506919.3
NBCI Gene record:
Kras (16653)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006506919.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000034385 CTATACATTAGTCCGAGAAAT pLKO.1 839 CDS 100% 13.200 10.560 N Kras n/a
2 TRCN0000301687 CTATACATTAGTCCGAGAAAT pLKO_005 839 CDS 100% 13.200 10.560 N Kras n/a
3 TRCN0000055355 CCTATGGTCCTGGTAGGGAAT pLKO.1 582 CDS 100% 4.050 3.240 N Kras n/a
4 TRCN0000033260 GAGGGCTTTCTTTGTGTATTT pLKO.1 480 CDS 100% 13.200 9.240 N KRAS n/a
5 TRCN0000363721 GAGGGCTTTCTTTGTGTATTT pLKO_005 480 CDS 100% 13.200 9.240 N KRAS n/a
6 TRCN0000055354 GTGTTGACGATGCCTTCTATA pLKO.1 823 CDS 100% 13.200 9.240 N Kras n/a
7 TRCN0000301812 GTGTTGACGATGCCTTCTATA pLKO_005 823 CDS 100% 13.200 9.240 N Kras n/a
8 TRCN0000369099 TGAAGATATTCACCATTATAG pLKO_005 524 CDS 100% 13.200 9.240 N KRAS n/a
9 TRCN0000034386 CCTACAGGAAACAAGTAGTAA pLKO.1 370 CDS 100% 5.625 3.938 N Kras n/a
10 TRCN0000055356 GAGAAACCTGTCTCTTGGATA pLKO.1 397 CDS 100% 4.950 3.465 N Kras n/a
11 TRCN0000055357 CAAGTAGTAATTGATGGAGAA pLKO.1 381 CDS 100% 4.050 2.835 N Kras n/a
12 TRCN0000301869 CAAGTAGTAATTGATGGAGAA pLKO_005 381 CDS 100% 4.050 2.835 N Kras n/a
13 TRCN0000034387 CTCAGGAGTTAGCAAGGAGTT pLKO.1 643 CDS 100% 4.050 2.835 N Kras n/a
14 TRCN0000301612 CTCAGGAGTTAGCAAGGAGTT pLKO_005 643 CDS 100% 4.050 2.835 N Kras n/a
15 TRCN0000377271 CCTTGACGATACAGCTAATTC pLKO_005 307 CDS 100% 13.200 7.920 N KRAS n/a
16 TRCN0000034384 GCCTTGACGATACAGCTAATT pLKO.1 306 CDS 100% 13.200 7.920 N Kras n/a
17 TRCN0000034388 GATGAGCAAAGATGGGAAGAA pLKO.1 878 CDS 100% 4.950 2.970 N Kras n/a
18 TRCN0000301688 GATGAGCAAAGATGGGAAGAA pLKO_005 878 CDS 100% 4.950 2.970 N Kras n/a
19 TRCN0000040149 GATGCCTTCTATACATTAGTT pLKO.1 831 CDS 100% 5.625 3.375 N KRAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006506919.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488169 GAACCACTCCTATCCCCCATGTTT pLX_317 59.8% 79% 81.9% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000488343 CTATAAAAACTCAAGTCCGGTCCC pLX_317 54.9% 77.5% 80.6% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_16173 pDONR223 0% 77.3% 80.1% None (many diffs) n/a
4 ccsbBroad304_16173 pLX_304 0% 77.3% 80.1% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489180 TCGGCGACTATGAATTTCTAAAAT pLX_317 67.2% 77.3% 80.1% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000487752 TAGTTCACCCCAGACCCGAATCAA pLX_317 36.1% 77.2% 79.8% V5 (many diffs) n/a
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