Construct: ORF ccsbBroad304_99985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORFC00001.1_s304c1, BRDN0000412222, pLX304-BFP
- Derived from:
- BRDN0000464762
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BFP (BFP)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_99985
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | BFP | BFP | Hahn Lab BFP | BFP.1 | 99.8% | 99.5% | 716A>N |
| 2 | CONTROL | eGFP | eGFP | Hahn Lab eGFP | eGFP.1 | 99% | 97.9% | (many diffs) |
| 3 | CONTROL | GFP | GFP | clonetechGfp | clonetechGfp.1 | 99% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 786
- ORF length:
- 717
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 121 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 181 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 241 ggcccaccct cgtgaccacc cTGAGCCACG GCGTCCAGTG CTTCAGCCGC TACCCCGACC 301 ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA 361 CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG 421 ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC 481 TGGGGCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT CTATATCATG GCCGACAAGC 541 AGAAGAACGG CATCAAGGCG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC 601 AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG 661 ACAGCCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC 721 ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT 781 ACANGNNGCC NANTNTNTTN TGATTGCCTT TTTAAAAAAN AAAGTGGTGG GTACCCCCCC 841 CCCCCCCCCT CTCTTTGGTT TCTATTTAAG AAAATAATAA GGAACACCAG TGGGGTGTTA 901 AGTAAAGTCA ATCATCAACT TGAAATAAAA AAAGAAAAGA AAGAAATGGT ATTTTTATTA 961 ATTTTGCTCC TCTTATGCTA TGAGGATAAT ACGCTAATAT AATGCAAAAT ATTTCTTTAA 1021 ATA