Construct: ORF ccsbBroad304_99987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORFC00001.3_s304c1, BRDN0000412224
- Derived from:
- BRDN0000464762
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BFP (BFP)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_99987
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | BFP | BFP | Hahn Lab BFP | BFP.1 | 99.7% | 23.8% | 99delG;116delC |
| 2 | CONTROL | eGFP | eGFP | Hahn Lab eGFP | eGFP.1 | 98.8% | 23.5% | (many diffs) |
| 3 | CONTROL | GFP | GFP | clonetechGfp | clonetechGfp.1 | 98.8% | 23.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 786
- ORF length:
- 717
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 121 agctggacgg cgacgtaaaC GGCCACAAGT TCAGCGTGTC CGGCGAGGCG AGGGCGATGC 181 CACTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG 241 CCCACCCTCG TGACCACCCT GAGCCACGGC GTCCAGTGCT TCAGCCGCTA CCCCGACCAC 301 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC 361 ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC 421 ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG 481 GGGCACAAGC TGGAGTACAA CTTCAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG 541 AAGAACGGCA TCAAGGCGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG 601 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC 661 AGCCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC 721 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC 781 AAGTTGCCAA CTTTCTTGTA Caaagtggtt ggtaagccta tccctaaccc tctcctcggt 841 ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac 901 ctctggatta