Construct: ORF ccsbBroadEn_00043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004804.1_s300c1, BRDN0000388781
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIF1 (199)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 199 | AIF1 | allograft inflammatory fact... | NM_001623.5 | 100% | 100% | |
| 2 | human | 199 | AIF1 | allograft inflammatory fact... | NM_001318970.2 | 63.2% | 63.2% | 0_1ins162 |
| 3 | human | 199 | AIF1 | allograft inflammatory fact... | NM_032955.3 | 63.2% | 63.2% | 0_1ins162 |
| 4 | human | 199 | AIF1 | allograft inflammatory fact... | XM_005248870.4 | 56.3% | 56.3% | 196_393del;558_559ins81 |
| 5 | human | 199 | AIF1 | allograft inflammatory fact... | XM_017010332.1 | 48.9% | 30.9% | (many diffs) |
| 6 | mouse | 11629 | Aif1 | allograft inflammatory fact... | NM_019467.2 | 89.1% | 89.1% | (many diffs) |
| 7 | mouse | 11629 | Aif1 | allograft inflammatory fact... | XM_006523503.3 | 89.1% | 89.1% | (many diffs) |
| 8 | mouse | 11629 | Aif1 | allograft inflammatory fact... | XM_006523504.3 | 89.1% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAG CCAAACCAGG GATTTACAGG GAGGAAAAGC TTTCGGACTG CTGAAGGCCC 121 AGCAGGAAGA GAGGCTGGAT GAGATCAACA AGCAATTCCT AGACGATCCC AAATATAGCA 181 GTGATGAGGA TCTGCCCTCC AAACTGGAAG GCTTCAAAGA GAAATACATG GAGTTTGACC 241 TTAATGGAAA TGGCGATATT GATATCATGT CCCTGAAACG AATGCTGGAG AAACTTGGAG 301 TCCCCAAGAC TCACCTAGAG CTAAAGAAAT TAATTGGAGA GGTGTCCAGT GGCTCCGGGG 361 AGACGTTCAG CTACCCTGAC TTTCTCAGGA TGATGCTGGG CAAGAGATCT GCCATCCTAA 421 AAATGATCCT GATGTATGAG GAAAAAGCGA GAGAAAAGGA AAAGCCAACA GGCCCCCCAG 481 CCAAGAAAGC TATCTCTGAG TTGCCCTGGC CCACTTTCTT GTACAAAGTt ggcattataa 541 gaaagcattg cttatcaatt tgttgcaacg aac