Construct: ORF ccsbBroadEn_00065

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011871.1_s300c1, BRDN0000388440
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AMY2A (279)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 279 AMY2A amylase alpha 2A NM_000699.3 100% 100%
2 human 280 AMY2B amylase alpha 2B NM_020978.4 98.3% 98.8% (many diffs)
3 human 277 AMY1B amylase alpha 1B NM_001008218.1 97.6% 96.8% (many diffs)
4 human 278 AMY1C amylase alpha 1C NM_001008219.2 97.6% 96.8% (many diffs)
5 human 276 AMY1A amylase alpha 1A NM_001008221.1 97.6% 96.8% (many diffs)
6 human 278 AMY1C amylase alpha 1C NM_001346780.1 97.6% 96.8% (many diffs)
7 human 276 AMY1A amylase alpha 1A NM_004038.3 97.6% 96.8% (many diffs)
8 human 277 AMY1B amylase alpha 1B XM_011541262.1 97.6% 96.8% (many diffs)
9 human 278 AMY1C amylase alpha 1C XM_017001058.1 97.6% 96.8% (many diffs)
10 mouse 109959 Amy2a5 amylase 2a5 NM_001042711.2 83.8% 85.1% (many diffs)
11 mouse 100043684 Amy2a4 amylase 2a4 NM_001160150.1 83.8% 85.1% (many diffs)
12 mouse 100043686 Amy2a3 amylase 2a3 NM_001160151.1 83.8% 85.1% (many diffs)
13 mouse 100043688 Amy2a2 amylase 2a2 NM_001160152.1 83.8% 85.1% (many diffs)
14 mouse 545562 Amy2b amylase 2b NM_001190403.1 74.9% 76.1% (many diffs)
15 mouse 545562 Amy2b amylase 2b NM_001190404.1 71.2% 72.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1599
ORF length:
1533
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA GTTCTTTCTG TTGCTTTTCA CCATTGGGTT CTGCTGGGCT CAGTATTCCC
121CAAATACACA ACAAGGACGG ACATCTATTG TTCATCTGTT TGAATGGCGA TGGGTTGATA
181TTGCTCTTGA ATGTGAGCGA TATTTAGCTC CGAAGGGATT TGGAGGGGTT CAGGTCTCTC
241CACCAAATGA AAATGTTGCA ATTTACAACC CTTTCAGACC TTGGTGGGAA AGATACCAAC
301CAGTTAGCTA TAAATTATGC ACAAGATCTG GAAATGAAGA TGAATTTAGA AACATGGTGA
361CTAGATGTAA CAATGTTGGG GTTCGTATTT ATGTGGATGC TGTAATTAAT CATATGTGTG
421GTAACGCTGT GAGTGCAGGA ACAAGCAGTA CCTGTGGAAG TTACTTCAAC CCTGGAAGTA
481GGGACTTTCC AGCAGTCCCA TATTCTGGAT GGGATTTCAA TGATGGTAAA TGTAAAACTG
541GAAGTGGAGA TATCGAGAAC TACAATGATG CTACTCAGGT CAGAGATTGT CGTCTGACTG
601GTCTTCTTGA TCTTGCACTG GAGAAGGATT ACGTGCGTTC TAAGATTGCC GAATATATGA
661ACCATCTCAT TGACATTGGT GTTGCAGGGT TCAGACTTGA TGCTTCCAAG CACATGTGGC
721CTGGAGACAT AAAGGCAATT TTGGACAAAC TGCATAATCT AAACAGTAAC TGGTTCCCTG
781CAGGAAGTAA ACCTTTCATT TACCAGGAGG TAATTGATCT GGGTGGTGAG CCAATTAAAA
841GCAGTGACTA CTTTGGTAAT GGCCGGGTGA CAGAATTCAA GTATGGTGCA AAACTCGGCA
901CAGTTATTCG CAAGTGGAAT GGAGAGAAGA TGTCTTACTT AAAGAACTGG GGAGAAGGTT
961GGGGTTTCGT ACCTTCTGAC AGAGCGCTTG TCTTTGTGGA TAACCATGAC AATCAACGAG
1021GACATGGGGC TGGAGGAGCC TCTATTCTTA CCTTCTGGGA TGCTAGGCTG TACAAAATGG
1081CAGTTGGATT TATGCTTGCT CATCCTTACG GATTTACACG AGTAATGTCA AGCTACCGTT
1141GGCCAAGACA GTTTCAAAAT GGAAACGATG TTAATGATTG GGTTGGGCCA CCAAATAATA
1201ATGGAGTAAT TAAAGAAGTT ACTATTAATC CAGACACTAC TTGTGGCAAT GACTGGGTCT
1261GTGAACATCG ATGGCGCCAA ATAAGGAACA TGGTTATTTT CCGCAATGTA GTGGATGGCC
1321AGCCTTTTAC AAATTGGTAT GATAATGGGA GCAACCAAGT GGCTTTTGGG AGAGGAAACA
1381GAGGATTCAT TGTTTTCAAC AATGATGACT GGTCATTTTC TTTAACTTTG CAAACTGGTC
1441TTCCTGCTGG CACATACTGT GATGTCATTT CTGGAGATAA AATTAATGGC AATTGCACAG
1501GCATTAAAAT TTACGTTTCT GATGATGGCA AAGCTCATTT TTCTATTAGT AACTCTGCTG
1561AAGATCCATT TATTGCAATT CATGCTGAAT CTAAATTGTA CCCAACTTTC TTGTACAAAG
1621Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac

Download FASTA (ORF) (Full)

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