Construct: ORF ccsbBroadEn_00065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011871.1_s300c1, BRDN0000388440
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMY2A (279)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 279 | AMY2A | amylase alpha 2A | NM_000699.3 | 100% | 100% | |
| 2 | human | 280 | AMY2B | amylase alpha 2B | NM_020978.4 | 98.3% | 98.8% | (many diffs) |
| 3 | human | 277 | AMY1B | amylase alpha 1B | NM_001008218.1 | 97.6% | 96.8% | (many diffs) |
| 4 | human | 278 | AMY1C | amylase alpha 1C | NM_001008219.2 | 97.6% | 96.8% | (many diffs) |
| 5 | human | 276 | AMY1A | amylase alpha 1A | NM_001008221.1 | 97.6% | 96.8% | (many diffs) |
| 6 | human | 278 | AMY1C | amylase alpha 1C | NM_001346780.1 | 97.6% | 96.8% | (many diffs) |
| 7 | human | 276 | AMY1A | amylase alpha 1A | NM_004038.3 | 97.6% | 96.8% | (many diffs) |
| 8 | human | 277 | AMY1B | amylase alpha 1B | XM_011541262.1 | 97.6% | 96.8% | (many diffs) |
| 9 | human | 278 | AMY1C | amylase alpha 1C | XM_017001058.1 | 97.6% | 96.8% | (many diffs) |
| 10 | mouse | 109959 | Amy2a5 | amylase 2a5 | NM_001042711.2 | 83.8% | 85.1% | (many diffs) |
| 11 | mouse | 100043684 | Amy2a4 | amylase 2a4 | NM_001160150.1 | 83.8% | 85.1% | (many diffs) |
| 12 | mouse | 100043686 | Amy2a3 | amylase 2a3 | NM_001160151.1 | 83.8% | 85.1% | (many diffs) |
| 13 | mouse | 100043688 | Amy2a2 | amylase 2a2 | NM_001160152.1 | 83.8% | 85.1% | (many diffs) |
| 14 | mouse | 545562 | Amy2b | amylase 2b | NM_001190403.1 | 74.9% | 76.1% | (many diffs) |
| 15 | mouse | 545562 | Amy2b | amylase 2b | NM_001190404.1 | 71.2% | 72.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1599
- ORF length:
- 1533
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA GTTCTTTCTG TTGCTTTTCA CCATTGGGTT CTGCTGGGCT CAGTATTCCC 121 CAAATACACA ACAAGGACGG ACATCTATTG TTCATCTGTT TGAATGGCGA TGGGTTGATA 181 TTGCTCTTGA ATGTGAGCGA TATTTAGCTC CGAAGGGATT TGGAGGGGTT CAGGTCTCTC 241 CACCAAATGA AAATGTTGCA ATTTACAACC CTTTCAGACC TTGGTGGGAA AGATACCAAC 301 CAGTTAGCTA TAAATTATGC ACAAGATCTG GAAATGAAGA TGAATTTAGA AACATGGTGA 361 CTAGATGTAA CAATGTTGGG GTTCGTATTT ATGTGGATGC TGTAATTAAT CATATGTGTG 421 GTAACGCTGT GAGTGCAGGA ACAAGCAGTA CCTGTGGAAG TTACTTCAAC CCTGGAAGTA 481 GGGACTTTCC AGCAGTCCCA TATTCTGGAT GGGATTTCAA TGATGGTAAA TGTAAAACTG 541 GAAGTGGAGA TATCGAGAAC TACAATGATG CTACTCAGGT CAGAGATTGT CGTCTGACTG 601 GTCTTCTTGA TCTTGCACTG GAGAAGGATT ACGTGCGTTC TAAGATTGCC GAATATATGA 661 ACCATCTCAT TGACATTGGT GTTGCAGGGT TCAGACTTGA TGCTTCCAAG CACATGTGGC 721 CTGGAGACAT AAAGGCAATT TTGGACAAAC TGCATAATCT AAACAGTAAC TGGTTCCCTG 781 CAGGAAGTAA ACCTTTCATT TACCAGGAGG TAATTGATCT GGGTGGTGAG CCAATTAAAA 841 GCAGTGACTA CTTTGGTAAT GGCCGGGTGA CAGAATTCAA GTATGGTGCA AAACTCGGCA 901 CAGTTATTCG CAAGTGGAAT GGAGAGAAGA TGTCTTACTT AAAGAACTGG GGAGAAGGTT 961 GGGGTTTCGT ACCTTCTGAC AGAGCGCTTG TCTTTGTGGA TAACCATGAC AATCAACGAG 1021 GACATGGGGC TGGAGGAGCC TCTATTCTTA CCTTCTGGGA TGCTAGGCTG TACAAAATGG 1081 CAGTTGGATT TATGCTTGCT CATCCTTACG GATTTACACG AGTAATGTCA AGCTACCGTT 1141 GGCCAAGACA GTTTCAAAAT GGAAACGATG TTAATGATTG GGTTGGGCCA CCAAATAATA 1201 ATGGAGTAAT TAAAGAAGTT ACTATTAATC CAGACACTAC TTGTGGCAAT GACTGGGTCT 1261 GTGAACATCG ATGGCGCCAA ATAAGGAACA TGGTTATTTT CCGCAATGTA GTGGATGGCC 1321 AGCCTTTTAC AAATTGGTAT GATAATGGGA GCAACCAAGT GGCTTTTGGG AGAGGAAACA 1381 GAGGATTCAT TGTTTTCAAC AATGATGACT GGTCATTTTC TTTAACTTTG CAAACTGGTC 1441 TTCCTGCTGG CACATACTGT GATGTCATTT CTGGAGATAA AATTAATGGC AATTGCACAG 1501 GCATTAAAAT TTACGTTTCT GATGATGGCA AAGCTCATTT TTCTATTAGT AACTCTGCTG 1561 AAGATCCATT TATTGCAATT CATGCTGAAT CTAAATTGTA CCCAACTTTC TTGTACAAAG 1621 Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac