Construct: ORF ccsbBroadEn_00517
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014745.1_s300c1, BRDN0000394948
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ESR1 (2099)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2099 | ESR1 | estrogen receptor 1 | NM_000125.3 | 100% | 100% | |
| 2 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001122740.1 | 100% | 100% | |
| 3 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001122741.1 | 100% | 100% | |
| 4 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001122742.1 | 100% | 100% | |
| 5 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535543.2 | 99.8% | 100% | 729T>C;975G>C |
| 6 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535544.2 | 99.8% | 100% | 729T>C;975G>C |
| 7 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535545.2 | 99.8% | 100% | 729T>C;975G>C |
| 8 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010376.1 | 99.8% | 100% | 729T>C;975G>C |
| 9 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010377.1 | 99.8% | 100% | 729T>C;975G>C |
| 10 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010378.1 | 99.8% | 100% | 729T>C;975G>C |
| 11 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010379.1 | 99.8% | 100% | 729T>C;975G>C |
| 12 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010380.1 | 99.8% | 100% | 729T>C;975G>C |
| 13 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010381.1 | 99.8% | 100% | 729T>C;975G>C |
| 14 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001291241.1 | 99.8% | 99.8% | 756_757insGGT |
| 15 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001291230.1 | 99.6% | 99.6% | 644_649delGTAATA |
| 16 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535547.2 | 79.5% | 76.4% | (many diffs) |
| 17 | human | 2099 | ESR1 | estrogen receptor 1 | XM_006715374.3 | 78.2% | 76.8% | (many diffs) |
| 18 | human | 2099 | ESR1 | estrogen receptor 1 | XM_006715375.3 | 70.8% | 70.9% | 0_1ins519;210T>C;456G>C |
| 19 | human | 2099 | ESR1 | estrogen receptor 1 | XR_002956266.1 | 65.5% | (many diffs) | |
| 20 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010382.2 | 63% | 63.1% | 0_1ins657;72T>C;318G>C |
| 21 | human | 2099 | ESR1 | estrogen receptor 1 | XM_011535549.2 | 58% | 57.1% | (many diffs) |
| 22 | human | 2099 | ESR1 | estrogen receptor 1 | XM_017010383.1 | 55.7% | 55.7% | 0_1ins789;186G>C |
| 23 | human | 2099 | ESR1 | estrogen receptor 1 | XR_001743223.2 | 52.9% | (many diffs) | |
| 24 | human | 2099 | ESR1 | estrogen receptor 1 | NM_001328100.2 | 50.6% | 47.9% | (many diffs) |
| 25 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | NM_001302531.1 | 85.6% | 88.9% | (many diffs) |
| 26 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | NM_001302532.1 | 85.6% | 88.9% | (many diffs) |
| 27 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | NM_007956.5 | 85.6% | 88.9% | (many diffs) |
| 28 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_006512433.3 | 85.6% | 88.9% | (many diffs) |
| 29 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_006512434.3 | 85.6% | 88.9% | (many diffs) |
| 30 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_006512435.2 | 85.6% | 88.9% | (many diffs) |
| 31 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_011243066.2 | 85.6% | 88.9% | (many diffs) |
| 32 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_011243067.2 | 85.6% | 88.9% | (many diffs) |
| 33 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_011243068.2 | 85.6% | 88.9% | (many diffs) |
| 34 | mouse | 13982 | Esr1 | estrogen receptor 1 (alpha) | XM_017313797.1 | 54.2% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1854
- ORF length:
- 1785
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GACCATGACC CTCCACACCA AAGCATCTGG GATGGCCCTA CTGCATCAGA 121 TCCAAGGGAA CGAGCTGGAG CCCCTGAACC GTCCGCAGCT CAAGATCCCC CTGGAGCGGC 181 CCCTGGGCGA GGTGTACCTG GACAGCAGCA AGCCCGCCGT GTACAACTAC CCCGAGGGCG 241 CCGCCTACGA GTTCAACGCC GCGGCCGCCG CCAACGCGCA GGTCTACGGT CAGACCGGCC 301 TCCCCTACGG CCCCGGGTCT GAGGCTGCGG CGTTCGGCTC CAACGGCCTG GGGGGTTTCC 361 CCCCACTCAA CAGCGTGTCT CCGAGCCCGC TGATGCTACT GCACCCGCCG CCGCAGCTGT 421 CGCCTTTCCT GCAGCCCCAC GGCCAGCAGG TGCCCTACTA CCTGGAGAAC GAGCCCAGCG 481 GCTACACGGT GCGCGAGGCC GGCCCGCCGG CATTCTACAG GCCAAATTCA GATAATCGAC 541 GCCAGGGTGG CAGAGAAAGA TTGGCCAGTA CCAATGACAA GGGAAGTATG GCTATGGAAT 601 CTGCCAAGGA GACTCGCTAC TGTGCAGTGT GCAATGACTA TGCTTCAGGC TACCATTATG 661 GAGTCTGGTC CTGTGAGGGC TGCAAGGCCT TCTTCAAGAG AAGTATTCAA GGACATAACG 721 ACTATATGTG TCCAGCCACC AACCAGTGCA CCATTGATAA AAACAGGAGG AAGAGCTGCC 781 AGGCCTGCCG GCTCCGCAAA TGCTACGAAG TGGGAATGAT GAAAGGTGGG ATACGAAAAG 841 ACCGAAGAGG AGGGAGAATG TTGAAACACA AGCGCCAGAG AGATGATGGG GAGGGCAGGG 901 GTGAAGTGGG GTCTGCTGGA GACATGAGAG CTGCCAACCT TTGGCCAAGC CCGCTCATGA 961 TCAAACGCTC TAAGAAGAAC AGCCTGGCCT TGTCCCTGAC GGCCGACCAG ATGGTCAGTG 1021 CCTTGTTGGA TGCTGAGCCC CCCATACTCT ATTCCGAGTA TGATCCTACC AGACCCTTCA 1081 GTGAAGCTTC GATGATGGGC TTACTGACCA ACCTGGCAGA CAGGGAGCTG GTTCACATGA 1141 TCAACTGGGC GAAGAGGGTG CCAGGCTTTG TGGATTTGAC CCTCCATGAT CAGGTCCACC 1201 TTCTAGAATG TGCCTGGCTA GAGATCCTGA TGATTGGTCT CGTCTGGCGC TCCATGGAGC 1261 ACCCAGGGAA GCTACTGTTT GCTCCTAACT TGCTCTTGGA CAGGAACCAG GGAAAATGTG 1321 TAGAGGGCAT GGTGGAGATC TTCGACATGC TGCTGGCTAC ATCATCTCGG TTCCGCATGA 1381 TGAATCTGCA GGGAGAGGAG TTTGTGTGCC TCAAATCTAT TATTTTGCTT AATTCTGGAG 1441 TGTACACATT TCTGTCCAGC ACCCTGAAGT CTCTGGAAGA GAAGGACCAT ATCCACCGAG 1501 TCCTGGACAA GATCACAGAC ACTTTGATCC ACCTGATGGC CAAGGCAGGC CTGACCCTGC 1561 AGCAGCAGCA CCAGCGGCTG GCCCAGCTCC TCCTCATCCT CTCCCACATC AGGCACATGA 1621 GTAACAAAGG CATGGAGCAT CTGTACAGCA TGAAGTGCAA GAACGTGGTG CCCCTCTATG 1681 ACCTGCTGCT GGAGATGCTG GACGCCCACC GCCTACATGC GCCCACTAGC CGTGGAGGGG 1741 CATCCGTGGA GGAGACGGAC CAAAGCCACT TGGCCACTGC GGGCTCTACT TCATCGCATT 1801 CCTTGCAAAA GTATTACATC ACGGGGGAGG CAGAGGGTTT CCCTGCCACG GTCTTGCCAA 1861 CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac