Construct: ORF ccsbBroadEn_00566

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013541.1_s300c1, BRDN0000385798
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FHIT (2272)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2272 FHIT fragile histidine triad dia... NM_001166243.3 100% 100%
2 human 2272 FHIT fragile histidine triad dia... NM_001320899.2 100% 100%
3 human 2272 FHIT fragile histidine triad dia... NM_001320900.2 100% 100%
4 human 2272 FHIT fragile histidine triad dia... NM_002012.4 100% 100%
5 human 2272 FHIT fragile histidine triad dia... NM_001354589.2 84.3% 84.3% 277_278ins69
6 human 2272 FHIT fragile histidine triad dia... NM_001354590.2 84.3% 84.3% 277_278ins69
7 human 2272 FHIT fragile histidine triad dia... NM_001320901.2 61.4% 59.1% (many diffs)
8 human 2272 FHIT fragile histidine triad dia... NR_135491.2 6.9% (many diffs)
9 mouse 14198 Fhit fragile histidine triad gene NM_001308286.1 85.5% 89.3% (many diffs)
10 mouse 14198 Fhit fragile histidine triad gene NM_010210.3 85.5% 89.3% (many diffs)
11 mouse 14198 Fhit fragile histidine triad gene XM_006517927.3 85.5% 89.3% (many diffs)
12 mouse 14198 Fhit fragile histidine triad gene XM_011244732.2 85.5% 89.3% (many diffs)
13 mouse 14198 Fhit fragile histidine triad gene XM_017315847.1 85.5% 89.3% (many diffs)
14 mouse 14198 Fhit fragile histidine triad gene XM_006517924.1 69.3% 72.4% (many diffs)
15 mouse 14198 Fhit fragile histidine triad gene NM_001308285.1 60.2% 62.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
507
ORF length:
441
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC GTTCAGATTT GGCCAACATC TCATCAAGCC CTCTGTAGTG TTTCTCAAAA
121CAGAACTGTC CTTCGCTCTT GTGAATAGGA AACCTGTGGT ACCAGGACAT GTCCTTGTGT
181GCCCGCTGCG GCCAGTGGAG CGCTTCCATG ACCTGCGTCC TGATGAAGTG GCCGATTTGT
241TTCAGACGAC CCAGAGAGTC GGGACAGTGG TGGAAAAACA TTTCCATGGG ACCTCTCTCA
301CCTTTTCCAT GCAGGATGGC CCCGAAGCCG GACAGACTGT GAAGCACGTT CACGTCCATG
361TTCTTCCCAG GAAGGCTGGA GACTTTCACA GGAATGACAG CATCTATGAG GAGCTCCAGA
421AACATGACAA GGAGGACTTT CCTGCCTCTT GGAGATCAGA GGAGGAAATG GCAGCAGAAG
481CCGCAGCTCT GCGGGTCTAC TTTCAGTGCC CAACTTTCTT GTACAAAGTt ggcattataa
541gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)