Transcript: Human NR_135491.2

Homo sapiens fragile histidine triad diadenosine triphosphatase (FHIT), transcript variant 6, non-coding RNA.

Source:
NCBI, updated 2019-08-18
Taxon:
Homo sapiens (human)
Gene:
FHIT (2272)
Length:
5557
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_135491.2
NBCI Gene record:
FHIT (2272)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_135491.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000303423 TCATCTCACCATCCTGTATTC pLKO_005 3364 3UTR 100% 10.800 7.560 N FHIT n/a
2 TRCN0000303421 ACGTTCACGTCCATGTTCTTC pLKO_005 377 3UTR 100% 4.950 3.465 N FHIT n/a
3 TRCN0000051174 CTTGGAGATCAGAGGAGGAAA pLKO.1 547 3UTR 100% 4.950 3.465 N FHIT n/a
4 TRCN0000051173 GCTGGAGACTTTCACAGGAAT pLKO.1 406 3UTR 100% 4.950 3.465 N FHIT n/a
5 TRCN0000299004 GCTGGAGACTTTCACAGGAAT pLKO_005 406 3UTR 100% 4.950 3.465 N FHIT n/a
6 TRCN0000380185 GCTATTGCCAACCAGTTTGAA pLKO_005 3287 3UTR 100% 5.625 3.375 N FHIT n/a
7 TRCN0000303422 CATGGGACCTCTCTCACCTTT pLKO_005 316 3UTR 100% 4.950 2.970 N FHIT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_135491.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00566 pDONR223 100% 6.9% None (many diffs) n/a
2 ccsbBroad304_00566 pLX_304 0% 6.9% V5 (many diffs) n/a
3 TRCN0000468451 GGAAATTAGAAAAGTCTCATCAGG pLX_317 83.7% 6.9% V5 (many diffs) n/a
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