Transcript: Mouse XM_011244732.2

PREDICTED: Mus musculus fragile histidine triad gene (Fhit), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fhit (14198)
Length:
1124
CDS:
371..823

Additional Resources:

NCBI RefSeq record:
XM_011244732.2
NBCI Gene record:
Fhit (14198)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011244732.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415899 TGAGAGTAACTCAAACGATTC pLKO_005 821 CDS 100% 6.000 8.400 N Fhit n/a
2 TRCN0000080935 CGTGACCTACATCCTGATGAA pLKO.1 512 CDS 100% 4.950 6.930 N Fhit n/a
3 TRCN0000436982 GGCCGATTTGTTTCAAGTGAC pLKO_005 535 CDS 100% 4.050 5.670 N Fhit n/a
4 TRCN0000081331 CGATTTGTTTCAAGTGACCCA pLKO.1 538 CDS 100% 0.660 0.924 N LOC435386 n/a
5 TRCN0000427007 ATGAAGCTAACCACTCTTTAT pLKO_005 893 3UTR 100% 13.200 9.240 N Fhit n/a
6 TRCN0000080933 GCCTTGTACAAAGAACTTTAT pLKO.1 933 3UTR 100% 13.200 9.240 N Fhit n/a
7 TRCN0000080934 CGGAATGACAACATCTATGAT pLKO.1 695 CDS 100% 5.625 3.938 N Fhit n/a
8 TRCN0000080937 CAGACTGTGAAGCATGTACAT pLKO.1 638 CDS 100% 4.950 3.465 N Fhit n/a
9 TRCN0000080936 GTGAATAGGAAACCCGTTGTA pLKO.1 446 CDS 100% 4.950 3.465 N Fhit n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011244732.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00566 pDONR223 100% 85.5% 89.3% None (many diffs) n/a
2 ccsbBroad304_00566 pLX_304 0% 85.5% 89.3% V5 (many diffs) n/a
3 TRCN0000468451 GGAAATTAGAAAAGTCTCATCAGG pLX_317 83.7% 85.5% 89.3% V5 (many diffs) n/a
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