Construct: ORF ccsbBroadEn_00657
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017569.1_s300c1, BRDN0000389364
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNG4 (2786)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2786 | GNG4 | G protein subunit gamma 4 | NM_001098721.1 | 100% | 100% | |
| 2 | human | 2786 | GNG4 | G protein subunit gamma 4 | NM_001098722.2 | 100% | 100% | |
| 3 | human | 2786 | GNG4 | G protein subunit gamma 4 | NM_004485.4 | 100% | 100% | |
| 4 | human | 2786 | GNG4 | G protein subunit gamma 4 | XM_006711761.2 | 100% | 100% | |
| 5 | human | 2786 | GNG4 | G protein subunit gamma 4 | XM_011544167.2 | 100% | 100% | |
| 6 | mouse | 14706 | Gng4 | guanine nucleotide binding ... | NM_001302997.1 | 89.7% | 98.6% | (many diffs) |
| 7 | mouse | 14706 | Gng4 | guanine nucleotide binding ... | NM_010317.3 | 89.7% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 291
- ORF length:
- 225
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA AGAGGGCATG TCTAATAACA GCACCACTAG CATCTCCCAA GCCAGGAAAG 121 CTGTGGAGCA GCTAAAGATG GAAGCCTGTA TGGACAGGGT CAAGGTCTCC CAGGCAGCTG 181 CGGACCTCCT GGCCTACTGT GAAGCTCACG TGCGGGAAGA TCCTCTCATC ATTCCAGTGC 241 CTGCATCAGA AAACCCCTTT CGCGAGAAGA AGTTCTTTTG TACCATTCTC TACCCAACTT 301 TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac