Transcript: Human NM_004485.4

Homo sapiens G protein subunit gamma 4 (GNG4), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
GNG4 (2786)
Length:
4722
CDS:
71..298

Additional Resources:

NCBI RefSeq record:
NM_004485.4
NBCI Gene record:
GNG4 (2786)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004485.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000008805 CGGGAAGATCCTCTCATCATT pLKO.1 218 CDS 100% 5.625 4.500 N GNG4 n/a
2 TRCN0000278013 CGGGAAGATCCTCTCATCATT pLKO_005 218 CDS 100% 5.625 4.500 N GNG4 n/a
3 TRCN0000008806 GCTAAAGATGGAAGCCTGTAT pLKO.1 136 CDS 100% 4.950 3.465 N GNG4 n/a
4 TRCN0000277951 GCTAAAGATGGAAGCCTGTAT pLKO_005 136 CDS 100% 4.950 3.465 N GNG4 n/a
5 TRCN0000008804 GCAGTAATATAAGGACCTGTT pLKO.1 1397 3UTR 100% 4.050 2.835 N GNG4 n/a
6 TRCN0000277950 GCAGTAATATAAGGACCTGTT pLKO_005 1397 3UTR 100% 4.050 2.835 N GNG4 n/a
7 TRCN0000008807 TCATCATTCCAGTGCCTGCAT pLKO.1 231 CDS 100% 2.640 1.848 N GNG4 n/a
8 TRCN0000277952 TCATCATTCCAGTGCCTGCAT pLKO_005 231 CDS 100% 2.640 1.848 N GNG4 n/a
9 TRCN0000008808 CCACTAGCATCTCCCAAGCCA pLKO.1 99 CDS 100% 0.250 0.175 N GNG4 n/a
10 TRCN0000286066 CCACTAGCATCTCCCAAGCCA pLKO_005 99 CDS 100% 0.250 0.175 N GNG4 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3454 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000172742 GAGACAGAGTCTTGCTCTGTT pLKO.1 1607 3UTR 100% 0.495 0.248 Y C11orf44 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3454 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004485.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00657 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00657 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000470212 ACCTGTCAGCCCGGTTGATGGGTA pLX_317 100% 100% 100% V5 n/a
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