Construct: ORF ccsbBroadEn_01018

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008643.1_s300c1, BRDN0000392212
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MLF1 (4291)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4291 MLF1 myeloid leukemia factor 1 NM_022443.5 100% 100%
2 human 4291 MLF1 myeloid leukemia factor 1 NM_001369783.1 94.6% 94.6% 196_240del
3 human 4291 MLF1 myeloid leukemia factor 1 NM_001130156.3 90.6% 90.6% 0_1ins75
4 human 4291 MLF1 myeloid leukemia factor 1 NM_001130157.3 90.6% 90.6% 0_1ins75
5 human 4291 MLF1 myeloid leukemia factor 1 NM_001369784.1 90.6% 90.6% 0_1ins75
6 human 4291 MLF1 myeloid leukemia factor 1 NM_001195432.2 86.1% 84.3% (many diffs)
7 human 4291 MLF1 myeloid leukemia factor 1 NM_001195434.2 85.8% 85.8% 0_1ins75;121_165del
8 human 4291 MLF1 myeloid leukemia factor 1 NM_001369785.1 85.8% 85.8% 0_1ins75;121_165del
9 human 4291 MLF1 myeloid leukemia factor 1 XM_011512852.3 85.8% 85.8% 0_1ins75;121_165del
10 human 4291 MLF1 myeloid leukemia factor 1 NM_001369781.1 81.1% 78.7% (many diffs)
11 human 4291 MLF1 myeloid leukemia factor 1 NM_001195433.2 74.6% 74.6% 0_1ins75;204_205ins129
12 human 4291 MLF1 myeloid leukemia factor 1 NM_001369782.1 72.3% 72.3% 0_1ins222
13 mouse 17349 Mlf1 myeloid leukemia factor 1 NM_010801.3 85.1% 80.2% (many diffs)
14 mouse 17349 Mlf1 myeloid leukemia factor 1 NM_001039543.2 80.6% 76% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
870
ORF length:
804
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTT CAGGATGCTG AACAGCAGTT TTGAGGATGA CCCCTTCTTC TCTGAGTCCA
121TTCTTGCACA CCGAGAAAAT ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA
181GAGACTTGCT CAGTATCTCT GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG
241ATGGTGAAGA TTCTTTGACT CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG
301TGTCAAATAT GAGAAACTAT ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG
361ATCCAAATGG ACATTCATTT TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG
421AACCGCCAAA GGTTTTTCAG GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG
481AAACCAGGAA AGCAATGAGA GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC
541ATATCCATGA CCGAGCTCAT GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG
601AGGTCAACCA GGAGTTCATC AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT
661GGCAAAGTGA GGTTTTGAAG TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA
721GAAGTGTTGG CCATGAGAAT CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC
781AAAGTCCAGC CATTGAACAT GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA
841AAGGCTCATC TGTGAAAAGC AACAAAAAAT ACCCAACTTT CTTGTACAAA GTtggcatta
901taagaaagca ttgcttatca atttgttgca acgaac

Download FASTA (ORF) (Full)