Construct: ORF ccsbBroadEn_01091

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009752.1_s300c1, BRDN0000391684
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NFIB (4781)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4781 NFIB nuclear factor I B NM_005596.3 100% 100%
2 human 4781 NFIB nuclear factor I B NM_001369471.1 99.7% 99.5% 1057_1058insGTG
3 human 4781 NFIB nuclear factor I B NM_001369474.1 98.2% 97.3% (many diffs)
4 human 4781 NFIB nuclear factor I B NM_001369472.1 98.1% 98.3% (many diffs)
5 human 4781 NFIB nuclear factor I B NM_001369473.1 97.9% 97.8% (many diffs)
6 human 4781 NFIB nuclear factor I B NM_001369476.1 97.7% 97.6% 0_1ins28;2delT
7 human 4781 NFIB nuclear factor I B NM_001369477.1 95.4% 95.2% 560_561ins54;1003_1004insGTG
8 human 4781 NFIB nuclear factor I B NM_001190738.1 93.5% 92.3% (many diffs)
9 human 4781 NFIB nuclear factor I B NM_001369468.1 93.4% 93.2% (many diffs)
10 human 4781 NFIB nuclear factor I B NM_001369464.1 86.2% 85.4% 1244_1332del;1350_1461del
11 human 4781 NFIB nuclear factor I B NM_001369466.1 84.6% 83.9% (many diffs)
12 human 4781 NFIB nuclear factor I B NM_001190737.2 84.6% 84.2% (many diffs)
13 human 4781 NFIB nuclear factor I B NM_001369467.1 84.2% 83.3% (many diffs)
14 human 4781 NFIB nuclear factor I B XM_006716775.3 84% 83.5% (many diffs)
15 human 4781 NFIB nuclear factor I B XM_006716774.3 83.1% 82.7% (many diffs)
16 human 4781 NFIB nuclear factor I B NM_001369465.1 82.7% 82.1% (many diffs)
17 human 4781 NFIB nuclear factor I B NM_001369463.1 82.5% 82.1% (many diffs)
18 human 4781 NFIB nuclear factor I B NM_001369462.1 81.1% 80.1% (many diffs)
19 human 4781 NFIB nuclear factor I B NM_001369478.1 80.3% 80.4% (many diffs)
20 human 4781 NFIB nuclear factor I B NM_001369469.1 76.3% 75.5% 0_1ins144;1100_1188del;1206_1317del
21 human 4781 NFIB nuclear factor I B NM_001369461.1 74.5% 74.1% (many diffs)
22 human 4781 NFIB nuclear factor I B XM_005251467.3 73.4% 72.9% (many diffs)
23 human 4781 NFIB nuclear factor I B XM_006716773.3 73.2% 72.6% (many diffs)
24 human 4781 NFIB nuclear factor I B NM_001369460.1 72.1% 71.7% (many diffs)
25 human 4781 NFIB nuclear factor I B NM_001369475.1 70.8% 70% 336_337ins225;1019_1107del;1125_1236del
26 human 4781 NFIB nuclear factor I B NM_001369459.1 70.6% 69.9% (many diffs)
27 human 4781 NFIB nuclear factor I B NM_001369458.1 69.5% 68.9% (many diffs)
28 human 4781 NFIB nuclear factor I B NM_001369470.1 66.8% 66.4% (many diffs)
29 human 4781 NFIB nuclear factor I B NM_001369480.1 56.9% 55.4% (many diffs)
30 human 4781 NFIB nuclear factor I B NM_001369479.1 49.1% 47% (many diffs)
31 human 4781 NFIB nuclear factor I B NM_001369481.1 47.1% 45.2% (many diffs)
32 human 4781 NFIB nuclear factor I B NM_001282787.1 29.6% 29.2% (many diffs)
33 human 4781 NFIB nuclear factor I B XM_005251471.3 29.2% 28.7% (many diffs)
34 human 4781 NFIB nuclear factor I B XR_001746309.2 12.5% (many diffs)
35 human 4781 NFIB nuclear factor I B XR_001746308.2 12.5% (many diffs)
36 human 4781 NFIB nuclear factor I B NR_161383.1 12.2% (many diffs)
37 human 4781 NFIB nuclear factor I B NR_161385.1 9.9% (many diffs)
38 human 4781 NFIB nuclear factor I B NR_161382.1 9.9% (many diffs)
39 human 4781 NFIB nuclear factor I B NR_161384.1 9.7% (many diffs)
40 mouse 18028 Nfib nuclear factor I/B NM_008687.6 93.8% 99.2% (many diffs)
41 mouse 18028 Nfib nuclear factor I/B XM_017320021.1 90.8% 95.6% (many diffs)
42 mouse 18028 Nfib nuclear factor I/B XM_017320022.1 90.8% 95.6% (many diffs)
43 mouse 18028 Nfib nuclear factor I/B NM_001286131.1 80.9% 84.8% (many diffs)
44 mouse 18028 Nfib nuclear factor I/B NM_001286127.1 79.4% 83.6% (many diffs)
45 mouse 18028 Nfib nuclear factor I/B XM_006537654.2 78% 82.1% (many diffs)
46 mouse 18028 Nfib nuclear factor I/B NM_001113210.2 69.9% 73.6% (many diffs)
47 mouse 18028 Nfib nuclear factor I/B NM_001113209.2 68.8% 72.4% (many diffs)
48 mouse 18028 Nfib nuclear factor I/B XM_006537653.3 67.1% 70.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1326
ORF length:
1260
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAT GTATTCTCCC ATCTGTCTCA CTCAGGATGA ATTTCACCCA TTCATCGAGG
121CACTTCTTCC ACATGTCCGT GCAATTGCCT ATACTTGGTT CAACCTGCAG GCTCGAAAAC
181GCAAGTACTT TAAAAAGCAT GAGAAGCGAA TGTCAAAGGA TGAAGAAAGA GCAGTCAAAG
241ATGAGCTTCT CAGTGAAAAG CCTGAAATCA AACAGAAGTG GGCATCCAGG CTCCTTGCCA
301AACTGCGCAA AGATATTCGC CAGGAGTATC GAGAGGACTT TGTGCTCACC GTGACTGGCA
361AGAAGCACCC GTGCTGTGTC TTATCCAATC CCGACCAGAA GGGTAAGATT AGGAGAATCG
421ACTGCCTGCG ACAGGCAGAC AAAGTCTGGC GTCTGGATCT AGTCATGGTG ATCCTGTTCA
481AAGGCATCCC CTTGGAAAGT ACCGATGGAG AGCGGCTCAT GAAATCCCCA CATTGCACAA
541ACCCAGCACT TTGTGTCCAG CCACATCATA TCACAGTATC AGTTAAGGAG CTTGATTTGT
601TTTTGGCATA CTACGTGCAG GAGCAAGATT CTGGACAATC AGGAAGTCCA AGCCACAATG
661ATCCTGCCAA GAATCCTCCA GGTTACCTTG AGGATAGTTT TGTAAAATCT GGAGTCTTCA
721ATGTATCAGA ACTTGTAAGA GTATCCAGAA CGCCCATAAC CCAGGGAACT GGAGTCAACT
781TCCCAATTGG AGAAATCCCA AGCCAACCAT ACTATCATGA CATGAACTCG GGGGTCAATC
841TTCAGAGGTC TCTGTCTTCT CCACCAAGCA GCAAAAGACC CAAAACTATA TCCATAGATG
901AAAATATGGA ACCAAGTCCT ACAGGAGACT TTTACCCCTC TCCAAGTTCA CCAGCTGCTG
961GAAGTCGAAC ATGGCACGAA AGAGATCAAG ATATGTCTTC TCCGACTACT ATGAAGAAGC
1021CTGAAAAGCC ATTGTTCAGC TCTGCATCTC CACAGGATTC TTCCCCAAGA CTGAGCACTT
1081TCCCCCAGCA CCACCATCCC GGAATACCTG GAGTTGCACA CAGTGTCATC TCAACTCGAA
1141CTCCACCTCC ACCTTCACCG TTGCCATTTC CAACACAAGC TATCCTTCCT CCAGCCCCAT
1201CGAGCTACTT TTCTCATCCA ACAATCAGAT ATCCTCCCCA CCTGAATCCT CAGGATACTC
1261TGAAGAACTA TGTACCTTCT TATGACCCAT CCAGTCCACA AACCAGCCAG TCCTGGTACC
1321TGGGCTACCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt
1381gttgcaacga ac

Download FASTA (ORF) (Full)