Transcript: Mouse XM_006537653.3

PREDICTED: Mus musculus nuclear factor I/B (Nfib), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Nfib (18028)
Length:
8567
CDS:
373..2058

Additional Resources:

NCBI RefSeq record:
XM_006537653.3
NBCI Gene record:
Nfib (18028)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006537653.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273782 ACGTCCGTGCAATTGCCTATA pLKO_005 413 CDS 100% 10.800 15.120 N Nfib n/a
2 TRCN0000012088 CCACAACCATAGTATAAGAAA pLKO.1 2069 3UTR 100% 5.625 7.875 N Nfib n/a
3 TRCN0000273781 CCACAACCATAGTATAAGAAA pLKO_005 2069 3UTR 100% 5.625 7.875 N Nfib n/a
4 TRCN0000012092 CCAACCATACTATCATGACAT pLKO.1 1083 CDS 100% 4.950 6.930 N Nfib n/a
5 TRCN0000273780 CAACTTCCCAATCGGAGAAAT pLKO_005 1056 CDS 100% 13.200 10.560 N Nfib n/a
6 TRCN0000012089 CCATTTATTGAGGCACTTCTT pLKO.1 388 CDS 100% 4.950 3.960 N Nfib n/a
7 TRCN0000273732 CAGACCAGAAGGGTAAGATTA pLKO_005 671 CDS 100% 13.200 9.240 N Nfib n/a
8 TRCN0000273731 CCACATCACAGTATCAGTTAA pLKO_005 846 CDS 100% 13.200 9.240 N Nfib n/a
9 TRCN0000014680 CCGTGCTGTGTCTTATCCAAT pLKO.1 649 CDS 100% 4.950 3.465 N NFIB n/a
10 TRCN0000274065 CCGTGCTGTGTCTTATCCAAT pLKO_005 649 CDS 100% 4.950 3.465 N NFIB n/a
11 TRCN0000012090 CCTTCCAGCTACTTCTCTCAT pLKO.1 1477 CDS 100% 4.950 3.465 N Nfib n/a
12 TRCN0000012091 CCTGTTCAAAGGCATCCCTTT pLKO.1 753 CDS 100% 0.405 0.284 N Nfib n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006537653.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01091 pDONR223 100% 67.1% 70.7% None (many diffs) n/a
2 ccsbBroad304_01091 pLX_304 0% 67.1% 70.7% V5 (many diffs) n/a
3 TRCN0000468617 CCCGTGCAGTTGAGCGCTAGTGAT pLX_317 31.4% 67.1% 70.7% V5 (many diffs) n/a
Download CSV