Construct: ORF ccsbBroadEn_01263

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017541.1_s300c1, BRDN0000395992
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP2R1A (5518)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5518 PPP2R1A protein phosphatase 2 scaff... NM_014225.6 100% 100%
2 human 5518 PPP2R1A protein phosphatase 2 scaff... NM_001363656.2 69.6% 69.6% 0_1ins537
3 human 5518 PPP2R1A protein phosphatase 2 scaff... NR_033500.2 31.1% 1_45del;213_214ins101;1712_5251del
4 mouse 51792 Ppp2r1a protein phosphatase 2, regu... NM_016891.3 90.5% 99.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1833
ORF length:
1767
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGCGGCCGAC GGCGACGACT CGCTGTACCC CATCGCGGTG CTCATAGACG
121AACTCCGCAA TGAGGACGTT CAGCTTCGCC TCAACAGCAT CAAGAAGCTG TCCACCATCG
181CCTTGGCCCT TGGGGTTGAA AGGACCCGAA GTGAGCTTCT GCCTTTCCTT ACAGATACCA
241TCTATGATGA AGATGAGGTC CTCCTGGCCC TGGCAGAACA GCTGGGAACC TTCACTACCC
301TGGTGGGAGG CCCAGAGTAC GTGCACTGCC TGCTGCCACC GCTGGAGTCG CTGGCCACAG
361TGGAGGAGAC AGTGGTGCGG GACAAGGCAG TGGAGTCCTT ACGGGCCATC TCACACGAGC
421ACTCGCCCTC TGACCTGGAG GCGCACTTTG TGCCGCTAGT GAAGCGGCTG GCGGGCGGCG
481ACTGGTTCAC CTCCCGCACC TCGGCCTGCG GCCTCTTCTC CGTCTGCTAC CCCCGAGTGT
541CCAGTGCTGT GAAGGCGGAA CTTCGACAGT ACTTCCGGAA CCTGTGCTCA GATGACACCC
601CCATGGTGCG GCGGGCCGCA GCCTCCAAGC TGGGGGAGTT TGCCAAGGTG CTGGAGCTGG
661ACAACGTCAA GAGTGAGATC ATCCCCATGT TCTCCAACCT GGCCTCTGAC GAGCAGGACT
721CGGTGCGGCT GCTGGCGGTG GAGGCGTGCG TGAACATCGC CCAGCTTCTG CCCCAGGAGG
781ATCTGGAGGC CCTGGTGATG CCCACTCTGC GCCAGGCCGC TGAAGACAAG TCCTGGCGCG
841TCCGCTACAT GGTGGCTGAC AAGTTCACAG AGCTCCAGAA AGCAGTGGGG CCTGAGATCA
901CCAAGACAGA CCTGGTCCCT GCCTTCCAGA ACCTGATGAA AGACTGTGAG GCCGAGGTGA
961GGGCCGCAGC CTCCCACAAG GTCAAAGAGT TCTGTGAAAA CCTCTCAGCT GACTGTCGGG
1021AGAATGTGAT CATGTCCCAG ATCTTGCCCT GCATCAAGGA GCTGGTGTCC GATGCCAACC
1081AACATGTCAA GTCTGCCCTG GCCTCAGTCA TCATGGGTCT CTCTCCCATC TTGGGCAAAG
1141ACAACACCAT CGAGCACCTC TTGCCCCTCT TCCTGGCTCA GCTGAAGGAT GAGTGCCCTG
1201AGGTACGGCT GAACATCATC TCTAACCTGG ACTGTGTGAA CGAGGTGATT GGCATCCGGC
1261AGCTGTCCCA GTCCCTGCTC CCTGCCATTG TGGAGCTGGC TGAGGACGCC AAGTGGCGGG
1321TGCGGCTGGC CATCATTGAG TACATGCCCC TCCTGGCTGG ACAGCTGGGA GTGGAGTTCT
1381TTGATGAGAA ACTTAACTCC TTGTGCATGG CCTGGCTTGT GGATCATGTA TATGCCATCC
1441GCGAGGCAGC CACCAGCAAC CTGAAGAAGC TAGTGGAAAA GTTTGGGAAG GAGTGGGCCC
1501ATGCCACAAT CATCCCCAAG GTCTTGGCCA TGTCCGGAGA CCCCAACTAC CTGCACCGCA
1561TGACTACGCT CTTCTGCATC AATGTGCTGT CTGAGGTCTG TGGGCAGGAC ATCACCACCA
1621AGCACATGCT ACCCACGGTT CTGCGCATGG CTGGGGACCC GGTTGCCAAT GTCCGCTTCA
1681ATGTGGCCAA GTCTCTGCAG AAGATAGGGC CCATCCTGGA CAACAGCACC TTGCAGAGTG
1741AAGTCAAGCC CATCCTAGAG AAGCTGACCC AGGACCAGGA TGTGGACGTC AAATACTTTG
1801CCCAGGAGGC TCTGACTGTT CTGTCTCTCG CCTGCCCAAC TTTCTTGTAC AAAGTtggca
1861ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)

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