Construct: ORF ccsbBroadEn_01264

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014278.1_s300c1, BRDN0000386327
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP2R2B (5521)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5521 PPP2R2B protein phosphatase 2 regul... NM_001271948.1 96.7% 95% (many diffs)
2 human 5521 PPP2R2B protein phosphatase 2 regul... NM_181678.2 96.7% 95% (many diffs)
3 human 5521 PPP2R2B protein phosphatase 2 regul... NM_181676.2 95.9% 93.8% (many diffs)
4 human 5521 PPP2R2B protein phosphatase 2 regul... NM_181677.2 95.1% 94.5% (many diffs)
5 human 5521 PPP2R2B protein phosphatase 2 regul... NM_001271899.1 95.1% 92.5% (many diffs)
6 human 5521 PPP2R2B protein phosphatase 2 regul... NM_001271900.2 88.4% 88.4% 1_174del
7 human 5521 PPP2R2B protein phosphatase 2 regul... NM_181674.2 87% 87% 1_198del
8 human 5521 PPP2R2B protein phosphatase 2 regul... NM_181675.3 80.6% 80.6% 1_318del
9 human 5521 PPP2R2B protein phosphatase 2 regul... NR_073527.1 62.3% 1_351del;1681_2130del
10 human 5521 PPP2R2B protein phosphatase 2 regul... NR_073526.2 11.8% 1_686del;2016_11223del
11 mouse 72930 Ppp2r2b protein phosphatase 2, regu... NM_028392.3 90.8% 93.8% (many diffs)
12 mouse 72930 Ppp2r2b protein phosphatase 2, regu... NR_073583.1 49.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1395
ORF length:
1329
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA GGAGGACATT GATACCCGCA AAATCAACAA CAGTTTCCTG CGCGACCACA
121GCTATGCGAC CGAAGCTGAC ATTATCTCTA CGGTAGAATT CAACCACACG GGAGAATTAC
181TAGCGACAGG GGACAAGGGG GGTCGGGTTG TAATATTTCA ACGAGAGCAG GAGAGTAAAA
241ATCAGGTTCA TCGTAGGGGT GAATACAATG TTTACAGCAC ATTCCAGAGC CATGAACCCG
301AGTTCGATTA CCTGAAGAGT TTAGAAATAG AAGAAAAAAT CAATAAAATA AGATGGCTCC
361CCCAGCAGAA TGCAGCTTAC TTTCTTCTGT CTACTAATGA TAAAACTGTG AAGCTGTGGA
421AAGTCAGCGA GCGTGATAAG AGGCCAGAAG GCTACAATCT GAAAGATGAG GAGGGCCGGC
481TCCGGGATCC TGCCACCATC ACAACCCTGC GGGTGCCTGT CCTGAGACCC ATGGACCTGA
541TGGTGGAGGC CACCCCACGA AGAGTATTTG CCAACGCACA CACATATCAC ATCAACTCCA
601TATCTGTCAA CAGCGACTAT GAAACCTACA TGTCCGCTGA TGACCTGAGG ATTAACCTAT
661GGAACTTTGA AATAACCAAT CAAAGTTTTA ATATTGTGGA CATTAAGCCA GCCAACATGG
721AGGAGCTCAC GGAGGTGATC ACAGCAGCCG AGTTCCACCC CCATCATTGC AACACCTTCG
781TGTACAGCAG CAGCAAAGGG ACAATCCGGC TGTGTGACAT GCGGGCATCT GCCCTGTGTG
841ACAGGCACAC CAAATTTTTT GAAGAGCCGG AAGATCCAAG CAACAGATCA TTTTTCTCTG
901AAATTATCTC TTCGATTTCG GATGTGAAGT TCAGCCACAG TGGGAGGTAT ATCATGACCA
961GGGACTACTT GACCGTCAAA GTCTGGGATC TCAACATGGA AAACCGCCCC ATCGAGACTT
1021ACCAGGTTCA TGACTACCTC CGCAGCAAGC TGTGTTCCCT CTATGAAAAT GACTGCATTT
1081TTGATAAATT TGAGTGTGTG TGGAATGGGT CAGACAGTGT CATCATGACA GGCTCCTACA
1141ACAACTTCTT CAGGATGTTC GACAGAAACA CCAAGCGTGA TGTGACCCTT GAGGCTTCGA
1201GGGAAAACAG CAAGCCCCGG GCTATCCTCA AACCCCGAAA AGTGTGTGTG GGGGGCAAGC
1261GGAGAAAAGA CGAGATCAGT GTCGACAGTC TGGACTTTAG CAAAAAGATC TTGCATACAG
1321CTTGGCATCC TTCAGAAAAT ATTATAGCAG TGGCGGCTAC AAATAACCTA TATATATTCC
1381AGGACAAGGT TAACTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct
1441tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)