Construct: ORF ccsbBroadEn_01281

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000904.1_s300c1, BRDN0000398121
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKCA (5578)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5578 PRKCA protein kinase C alpha NM_002737.3 99.9% 99.8% 1702G>A
2 human 5578 PRKCA protein kinase C alpha XM_017024836.2 93.3% 92.5% (many diffs)
3 human 5578 PRKCA protein kinase C alpha XM_017024837.1 91.3% 89.5% (many diffs)
4 human 5578 PRKCA protein kinase C alpha XM_024450829.1 86.4% 85.8% (many diffs)
5 human 5578 PRKCA protein kinase C alpha XM_024450830.1 86.4% 85.8% (many diffs)
6 human 5578 PRKCA protein kinase C alpha XR_001752558.1 55.7% (many diffs)
7 human 5578 PRKCA protein kinase C alpha XM_017024841.1 47.1% 45.6% (many diffs)
8 mouse 18750 Prkca protein kinase C, alpha NM_011101.3 91% 99.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2085
ORF length:
2016
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCTGACGTT TTCCCGGGCA ACGACTCCAC GGCGTCTCAG GACGTGGCCA
121ACCGCTTCGC CCGCAAAGGG GCGCTGAGGC AGAAGAACGT GCACGAGGTG AAGGACCACA
181AATTCATCGC GCGCTTCTTC AAGCAGCCCA CCTTCTGCAG CCACTGCACC GACTTCATCT
241GGGGGTTTGG GAAACAAGGC TTCCAGTGCC AAGTTTGCTG TTTTGTGGTC CACAAGAGGT
301GCCATGAATT TGTTACTTTT TCTTGTCCGG GTGCGGATAA GGGACCCGAC ACTGATGACC
361CCAGGAGCAA GCACAAGTTC AAAATCCACA CTTACGGAAG CCCCACCTTC TGCGATCACT
421GTGGGTCACT GCTCTATGGA CTTATCCATC AAGGGATGAA ATGTGACACC TGCGATATGA
481ACGTTCACAA GCAATGCGTC ATCAATGTCC CCAGCCTCTG CGGAATGGAT CACACTGAGA
541AGAGGGGGCG GATTTACCTA AAGGCTGAGG TTGCTGATGA AAAGCTCCAT GTCACAGTAC
601GAGATGCAAA AAATCTAATC CCTATGGATC CAAACGGGCT TTCAGATCCT TATGTGAAGC
661TGAAACTTAT TCCTGATCCC AAGAATGAAA GCAAGCAAAA AACCAAAACC ATCCGCTCCA
721CACTAAATCC GCAGTGGAAT GAGTCCTTTA CATTCAAATT GAAACCTTCA GACAAAGACC
781GACGACTGTC TGTAGAAATC TGGGACTGGG ATCGAACAAC AAGGAATGAC TTCATGGGAT
841CCCTTTCCTT TGGAGTTTCG GAGCTGATGA AGATGCCGGC CAGTGGATGG TACAAGTTGC
901TTAACCAAGA AGAAGGTGAG TACTACAACG TACCCATTCC GGAAGGGGAC GAGGAAGGAA
961ACATGGAACT CAGGCAGAAA TTCGAGAAAG CCAAACTTGG CCCTGCTGGC AACAAAGTCA
1021TCAGTCCCTC TGAAGACAGG AAACAACCTT CCAACAACCT TGACCGAGTG AAACTCACGG
1081ACTTCAATTT CCTCATGGTG TTGGGAAAGG GGAGTTTTGG AAAGGTGATG CTTGCCGACA
1141GGAAGGGCAC AGAAGAACTG TATGCAATCA AAATCCTGAA GAAGGATGTG GTGATTCAGG
1201ATGATGACGT GGAGTGCACC ATGGTAGAAA AGCGAGTCTT GGCCCTGCTT GACAAACCCC
1261CGTTCTTGAC GCAGCTGCAC TCCTGCTTCC AGACAGTGGA TCGGCTGTAC TTCGTCATGG
1321AATATGTCAA CGGTGGGGAC CTCATGTACC ACATTCAGCA AGTAGGAAAA TTTAAGGAAC
1381CACAAGCAGT ATTCTATGCG GCAGAGATTT CCATCGGATT GTTCTTTCTT CATAAAAGAG
1441GAATCATTTA TAGGGATCTG AAGTTAGATA ACGTCATGTT GGATTCAGAA GGACATATCA
1501AAATTGCTGA CTTTGGGATG TGCAAGGAAC ACATGATGGA TGGAGTCACG ACCAGGACCT
1561TCTGTGGGAC TCCAGATTAT ATCGCCCCAG AGATAATCGC TTATCAGCCG TATGGAAAAT
1621CTGTGGACTG GTGGGCCTAT GGCGTCCTGT TGTATGAAAT GCTTGCCGGG CAGCCTCCAT
1681TTGATGGTGA AGATGAAGAC GAGCTATTTC AGTCTATCAT GGAGCACAAC GTTTCCTATC
1741CAAAATCCTT GTCCAAGGAG GCTGTTTCTA TCTGCAAAGG ACTGATGACC AAACACCCAG
1801CCAAGCGGCT GGGCTGTGGG CCTGAGGGGG AGAGGGACGT GAGAGAGCAT GCCTTCTTCC
1861GGAGGATCGA CTGGGAAAAA CTGGAGAACA GGGAGATCCA GCCACCATTC AAGCCCAAAG
1921TGTGTGGCAA AGGAGCAGAG AACTTTGACA AGTTCTTCAC ACGAGGACAG CCCGTCTTAA
1981CACCACCTGA TCAGCTGGTT ATTGCTAACA TAGACCAGTC TGATTTTGAA GGGTTCTCGT
2041ATGTCAACCC CCAGTTTGTG CACCCCATCT TACAGAGTGC AGTATTGCCA ACTTTCTTGT
2101ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)