Construct: ORF ccsbBroadEn_01362

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013559.1_s300c1, BRDN0000385661
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RAB27B (5874)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5874 RAB27B RAB27B, member RAS oncogene... NM_004163.4 100% 100%
2 human 5874 RAB27B RAB27B, member RAS oncogene... XM_005266733.1 100% 100%
3 human 5874 RAB27B RAB27B, member RAS oncogene... XM_017025913.1 100% 100%
4 human 5874 RAB27B RAB27B, member RAS oncogene... XM_017025914.1 100% 100%
5 human 5874 RAB27B RAB27B, member RAS oncogene... XM_024451232.1 100% 100%
6 human 5874 RAB27B RAB27B, member RAS oncogene... XM_006722518.1 78.4% 73.8% (many diffs)
7 human 5874 RAB27B RAB27B, member RAS oncogene... XM_011526131.1 78.4% 73.8% (many diffs)
8 human 5874 RAB27B RAB27B, member RAS oncogene... XR_935245.1 11.1% 1_521del;1176_5858del
9 mouse 80718 Rab27b RAB27B, member RAS oncogene... NM_001082553.2 88.9% 94.4% (many diffs)
10 mouse 80718 Rab27b RAB27B, member RAS oncogene... NM_001302798.1 88.9% 94.4% (many diffs)
11 mouse 80718 Rab27b RAB27B, member RAS oncogene... NM_030554.4 88.9% 94.4% (many diffs)
12 mouse 80718 Rab27b RAB27B, member RAS oncogene... NM_001302799.1 61.3% 61% (many diffs)
13 mouse 80718 Rab27b RAB27B, member RAS oncogene... XM_006526364.2 61.3% 61% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
720
ORF length:
654
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAC CGATGGAGAC TATGATTATC TGATCAAACT CCTGGCCCTC GGGGATTCAG
121GGGTGGGGAA GACAACATTT CTTTATAGAT ACACAGATAA TAAATTCAAT CCCAAATTCA
181TCACTACAGT AGGAATAGAC TTTCGGGAAA AACGTGTGGT TTATAATGCA CAAGGACCGA
241ATGGATCTTC AGGGAAAGCA TTTAAAGTGC ATCTTCAGCT TTGGGACACT GCGGGACAAG
301AGCGGTTCCG GAGTCTCACC ACTGCATTTT TCAGAGACGC CATGGGCTTC TTATTAATGT
361TTGACCTCAC CAGTCAACAG AGCTTCTTAA ATGTCAGAAA CTGGATGAGC CAACTGCAAG
421CAAATGCTTA TTGTGAAAAT CCAGATATAG TATTAATTGG CAACAAGGCA GACCTACCAG
481ATCAGAGGGA AGTCAATGAA CGGCAAGCTC GGGAACTGGC TGACAAATAT GGCATACCAT
541ATTTTGAAAC AAGTGCAGCA ACTGGACAGA ATGTGGAGAA AGCTGTAGAA ACCCTTTTGG
601ACTTAATCAT GAAGCGAATG GAACAGTGTG TGGAGAAGAC ACAAATCCCT GATACTGTCA
661ATGGTGGAAA TTCTGGAAAC TTGGATGGGG AAAAGCCACC AGAGAAGAAA TGTATCTGCT
721ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
781acgaac

Download FASTA (ORF) (Full)