Construct: ORF ccsbBroadEn_01393
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005965.1_s300c1, BRDN0000386596
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFX3 (5991)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5991 | RFX3 | regulatory factor X3 | NM_001282116.1 | 100% | 100% | |
| 2 | human | 5991 | RFX3 | regulatory factor X3 | NM_134428.3 | 100% | 100% | |
| 3 | human | 5991 | RFX3 | regulatory factor X3 | XM_006716843.2 | 97.2% | 97.2% | 1969_2031del |
| 4 | human | 5991 | RFX3 | regulatory factor X3 | XM_006716844.2 | 97.2% | 97.2% | 1969_2031del |
| 5 | human | 5991 | RFX3 | regulatory factor X3 | XM_006716845.2 | 97.2% | 97.2% | 1969_2031del |
| 6 | human | 5991 | RFX3 | regulatory factor X3 | XM_011517998.2 | 97.2% | 97.2% | 1969_2031del |
| 7 | human | 5991 | RFX3 | regulatory factor X3 | XM_011517999.2 | 97.2% | 97.2% | 1969_2031del |
| 8 | human | 5991 | RFX3 | regulatory factor X3 | XM_017015000.2 | 97.2% | 97.2% | 1969_2031del |
| 9 | human | 5991 | RFX3 | regulatory factor X3 | XM_024447634.1 | 97.2% | 97.2% | 1969_2031del |
| 10 | human | 5991 | RFX3 | regulatory factor X3 | XM_024447635.1 | 97.2% | 97.2% | 1969_2031del |
| 11 | human | 5991 | RFX3 | regulatory factor X3 | XM_017015001.1 | 96.6% | 96.6% | 474_475ins75 |
| 12 | human | 5991 | RFX3 | regulatory factor X3 | XM_024447632.1 | 96.5% | 96.5% | 1_18del;1987_2049del |
| 13 | human | 5991 | RFX3 | regulatory factor X3 | XM_006716840.2 | 96% | 96% | 1_30del;1999_2061del |
| 14 | human | 5991 | RFX3 | regulatory factor X3 | XM_024447633.1 | 94% | 94% | 474_475ins75;1894_1956del |
| 15 | human | 5991 | RFX3 | regulatory factor X3 | XM_024447630.1 | 93.6% | 93.6% | 1_90del;2059_2121del |
| 16 | human | 5991 | RFX3 | regulatory factor X3 | XM_024447631.1 | 93.6% | 93.6% | 1_90del;2059_2121del |
| 17 | human | 5991 | RFX3 | regulatory factor X3 | NM_002919.3 | 90.8% | 87.9% | (many diffs) |
| 18 | human | 5991 | RFX3 | regulatory factor X3 | XM_011518001.1 | 86.4% | 86.4% | 0_1ins249;1720_1782del |
| 19 | human | 5991 | RFX3 | regulatory factor X3 | XM_017015002.2 | 61.9% | 60% | (many diffs) |
| 20 | human | 5991 | RFX3 | regulatory factor X3 | XM_006716847.3 | 60.9% | 60.6% | (many diffs) |
| 21 | human | 5991 | RFX3 | regulatory factor X3 | NM_001282117.1 | 54.6% | 53.1% | (many diffs) |
| 22 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | NM_001166414.1 | 92.4% | 98.9% | (many diffs) |
| 23 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | NM_011265.3 | 92.4% | 98.9% | (many diffs) |
| 24 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_006526782.3 | 92.4% | 98.9% | (many diffs) |
| 25 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_011247169.2 | 92.4% | 98.9% | (many diffs) |
| 26 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_006526780.3 | 91.2% | 97.6% | (many diffs) |
| 27 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_006526778.3 | 89.3% | 95.6% | (many diffs) |
| 28 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_011247167.2 | 89.3% | 95.6% | (many diffs) |
| 29 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_006526784.3 | 89.2% | 95.5% | (many diffs) |
| 30 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_017318087.1 | 88.8% | 95% | (many diffs) |
| 31 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_017318086.1 | 87.6% | 93.7% | (many diffs) |
| 32 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_011247168.2 | 86.2% | 92.3% | (many diffs) |
| 33 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XM_006526785.3 | 63.9% | 67.8% | (many diffs) |
| 34 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XR_877668.2 | 25.4% | (many diffs) | |
| 35 | mouse | 19726 | Rfx3 | regulatory factor X, 3 (inf... | XR_001782562.1 | 21.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2313
- ORF length:
- 2247
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCA GACATCAGAG ACTGGGTCGG ACACAGGCTC GACAGTGACC TTACAAACAT 121 CTGTGGCTAG TCAAGCAGCA GTGCCTACGC AGGTGGTACA GCAAGTACCA GTACAACAAC 181 AGGTACAGCA GGTACAGACT GTGCAGCAGG TACAACATGT CTATCCCGCT CAGGTGCAGT 241 ATGTGGAAGG AAGCGATACT GTCTATACCA ATGGAGCAAT CCGAACAACA ACGTATCCTT 301 ACACAGAGAC ACAGATGTAC AGCCAAAATA CTGGAGGGAA TTACTTTGAT ACTCAAGGGA 361 GTTCCGCCCA GGTGACTACC GTGGTCTCAT CCCACAGTAT GGTGGGCACT GGTGGGATTC 421 AGATGGGCGT CACAGGAGGA CAACTCATCA GCAGCTCTGG AGGAACCTAT CTGATCGGCA 481 ACTCAATGGA GAATTCTGGT CACTCAGTGA CACACACAAC TCGGGCCTCC CCAGCGACAA 541 TTGAAATGGC GATTGAGACG CTGCAAAAGT CTGACGGTCT GTCCACTCAC AGAAGCTCTC 601 TTCTCAACAG CCATCTCCAG TGGCTGTTGG ACAATTATGA GACAGCAGAA GGAGTGAGCC 661 TTCCCAGAAG CACTCTGTAC AACCACTACC TTCGACACTG TCAGGAACAC AAACTGGACC 721 CAGTCAATGC TGCCTCTTTT GGAAAATTAA TAAGATCAAT TTTTATGGGG CTACGAACCA 781 GGAGATTGGG CACTAGAGGA AACTCCAAAT ACCACTACTA TGGGATTCGT GTCAAGCCAG 841 ATTCCCCTCT TAATCGTCTG CAAGAAGACA TGCAGTATAT GGCTATGAGA CAACAACCCA 901 TGCAACAGAA ACAAAGGTAC AAGCCTATGC AGAAAGTGGA TGGGGTTGCA GATGGTTTCA 961 CAGGAAGTGG TCAACAGACA GGCACATCTG TTGAGCAAAC TGTAATTGCC CAAAGCCAAC 1021 ATCATCAACA GTTTTTAGAT GCATCTCGAG CACTTCCAGA GTTTGGAGAA GTTGAAATCT 1081 CTTCTCTGCC AGATGGTACT ACCTTTGAGG ATATCAAGTC ACTGCAGAGT CTTTATAGAG 1141 AGCACTGTGA GGCAATATTG GACGTTGTTG TGAATCTTCA ATTTAGCCTG ATAGAAAAAT 1201 TGTGGCAAAC ATTCTGGCGC TATTCTCCCT CTACTCCAAC TGATGGCACT ACCATTACCG 1261 AATCGAGCAA TCTGAGTGAA ATAGAAAGTC GACTTCCGAA AGCAAAGCTG ATAACTCTGT 1321 GCAAACATGA GTCTATCCTG AAATGGATGT GTAACTGTGA CCATGGGATG TACCAGGCTT 1381 TGGTGGAGAT TCTCATCCCC GACGTCCTTA GACCTATTCC TAGTGCCTTG ACCCAAGCCA 1441 TTCGAAATTT TGCAAAAAGC CTTGAAGGTT GGCTTTCCAA TGCCATGAAC AATATTCCAC 1501 AGAGAATGAT ACAAACCAAG GTTGCCGCTG TAAGTGCCTT TGCCCAGACT CTGCGAAGAT 1561 ACACGTCGCT TAATCACCTG GCCCAGGCAG CTCGTGCAGT GCTTCAGAAC ACTTCCCAAA 1621 TCAACCAGAT GCTTAGTGAC CTCAACCGTG TCGACTTTGC CAATGTCCAG GAGCAGGCTT 1681 CCTGGGTGTG CCAGTGTGAT GACAACATGG TTCAGAGACT AGAAACAGAC TTCAAGATGA 1741 CTCTTCAGCA GCAGAGCACC CTGGAGCAGT GGGCTGCGTG GCTTGACAAT GTGATGATGC 1801 AAGCACTGAA ACCCTATGAA GGAAGACCCA GTTTTCCTAA AGCCGCCAGG CAGTTTCTGC 1861 TAAAATGGTC TTTCTACAGC TCAATGGTTA TTCGGGACTT AACCTTACGC AGTGCTGCTA 1921 GCTTTGGCTC CTTCCACCTG ATCCGTCTAC TCTACGACGA ATATATGTTT TACTTAGTAG 1981 AACATCGTGT TGCTCAGGCA ACAGGAGAGA CTCCTATAGC AGTCATGGGC GAGTTTGGTG 2041 ATTTAAATGC CGTGTCTCCT GGAAATCTGG ATAAAGATGA AGGCAGTGAA GTAGAAAGTG 2101 AAATGGATGA AGAACTGGAT GACTCTTCAG AGCCTCAAGC CAAAAGAGAG AAAACAGAGC 2161 TGAGCCAGGC ATTTCCAGTG GGCTGCATGC AGCCTGTTCT CGAGACTGGC GTGCAACCAA 2221 GCCTCCTGAA TCCAATTCAC AGCGAGCACA TTGTCACAAG TACTCAGACT ATCAGACAGT 2281 GCAGCGCTAC AGGAAATACC TACACTGCAG TCTACCCAAC TTTCTTGTAC AAAGTtggca 2341 ttataagaaa gcattgctta tcaatttgtt gcaacgaac