Transcript: Human XM_006716840.2

PREDICTED: Homo sapiens regulatory factor X3 (RFX3), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RFX3 (5991)
Length:
4370
CDS:
147..2489

Additional Resources:

NCBI RefSeq record:
XM_006716840.2
NBCI Gene record:
RFX3 (5991)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006716840.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232129 TCTACTCTACGACGAATATAT pLKO_005 2057 CDS 100% 15.000 21.000 N RFX3 n/a
2 TRCN0000014875 GCGAAGATACACGTCGCTTAA pLKO.1 1664 CDS 100% 10.800 15.120 N RFX3 n/a
3 TRCN0000232128 GCGAAGATACACGTCGCTTAA pLKO_005 1664 CDS 100% 10.800 15.120 N RFX3 n/a
4 TRCN0000014874 CCGAACAACAACGTATCCTTA pLKO.1 392 CDS 100% 4.950 6.930 N RFX3 n/a
5 TRCN0000014877 GCGACAATTGAAATGGCGATT pLKO.1 645 CDS 100% 4.050 5.670 N RFX3 n/a
6 TRCN0000232127 GACCCAAGCCATTCGAAATTT pLKO_005 1541 CDS 100% 15.000 10.500 N RFX3 n/a
7 TRCN0000429600 GACCCAAGCCATTCGAAATTT pLKO_005 1541 CDS 100% 15.000 10.500 N Rfx3 n/a
8 TRCN0000431736 TTGGCAGCAGATGCATCAAAT pLKO_005 2925 3UTR 100% 13.200 9.240 N Rfx3 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006716840.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01393 pDONR223 100% 96% 96% None 1_30del;1999_2061del n/a
2 ccsbBroad304_01393 pLX_304 0% 96% 96% V5 1_30del;1999_2061del n/a
3 ccsbBroadEn_11098 pDONR223 100% 52.4% 50.9% None (many diffs) n/a
4 ccsbBroad304_11098 pLX_304 0% 52.4% 50.9% V5 (many diffs) n/a
5 TRCN0000481511 AACTACTTAATTAAATATAGCAAC pLX_317 36.4% 52.4% 50.9% V5 (many diffs) n/a
Download CSV