Construct: ORF ccsbBroadEn_01616

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001418.1_s300c1, BRDN0000386125
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STX3 (6809)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6809 STX3 syntaxin 3 NM_004177.5 100% 100%
2 human 6809 STX3 syntaxin 3 XM_005274195.4 100% 100%
3 human 6809 STX3 syntaxin 3 XM_017018188.1 97% 96.5% 1_1delAins22;5T>A;6_7insGCC
4 human 6809 STX3 syntaxin 3 XM_017018189.1 97% 96.5% 1_1delAins22;5T>A;6_7insGCC
5 human 6809 STX3 syntaxin 3 XM_017018190.1 97% 96.5% 1_1delAins22;5T>A;6_7insGCC
6 human 6809 STX3 syntaxin 3 XM_017018191.1 97% 96.5% 1_1delAins22;5T>A;6_7insGCC
7 human 6809 STX3 syntaxin 3 XM_005274198.3 95.2% 93.7% (many diffs)
8 human 6809 STX3 syntaxin 3 NM_001178040.2 93.4% 92.3% (many diffs)
9 human 6809 STX3 syntaxin 3 XM_017018192.1 92.2% 90.3% (many diffs)
10 human 6809 STX3 syntaxin 3 XM_017018193.1 92.2% 90.3% (many diffs)
11 human 6809 STX3 syntaxin 3 XM_017018194.1 90.4% 88.9% (many diffs)
12 human 6809 STX3 syntaxin 3 XM_005274200.4 87.1% 87.1% 673_674ins111
13 human 6809 STX3 syntaxin 3 XM_017018195.1 84.1% 83.7% (many diffs)
14 human 6809 STX3 syntaxin 3 XM_011545221.2 66.4% 66.4% 0_1ins291
15 human 6809 STX3 syntaxin 3 XM_017018196.1 53.6% 53.6% 0_1ins291;382_383ins111
16 human 6809 STX3 syntaxin 3 XR_001747944.2 26.6% 1_228del;1096_3251del
17 mouse 20908 Stx3 syntaxin 3 NM_152220.2 91.6% 97.9% (many diffs)
18 mouse 20908 Stx3 syntaxin 3 NM_001025307.1 82.7% 87.9% (many diffs)
19 mouse 20908 Stx3 syntaxin 3 XM_006526844.3 78.9% 81.8% (many diffs)
20 mouse 20908 Stx3 syntaxin 3 XM_006526845.3 52.6% 51.8% (many diffs)
21 mouse 20908 Stx3 syntaxin 3 XM_017318108.1 52.6% 51.8% (many diffs)
22 mouse 20908 Stx3 syntaxin 3 XR_001782568.1 20.9% (many diffs)
23 mouse 20908 Stx3 syntaxin 3 XR_001782570.1 18.3% (many diffs)
24 mouse 20908 Stx3 syntaxin 3 XR_001782569.1 14.1% (many diffs)
25 mouse 20908 Stx3 syntaxin 3 XR_001782567.1 13.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
933
ORF length:
867
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA GGACCGTCTG GAGCAGCTGA AGGCCAAGCA GCTGACACAG GATGATGATA
121CTGATGCGGT TGAGATTGCT ATCGACAACA CGGCTTTTAT GGACGAGTTC TTTTCTGAGA
181TTGAGGAAAC TCGGCTTAAC ATTGACAAGA TCTCAGAACA TGTAGAGGAG GCTAAGAAAC
241TCTACAGTAT CATTCTCTCT GCACCGATTC CAGAGCCAAA AACCAAGGAT GACCTAGAGC
301AGCTCACGAC TGAGATTAAG AAAAGGGCCA ACAACGTCCG GAACAAACTG AAGAGCATGG
361AGAAGCATAT TGAAGAAGAT GAGGTCAGGT CATCGGCAGA CCTTCGGATT CGGAAATCCC
421AGCACTCTGT CCTTTCTCGG AAGTTTGTGG AGGTGATGAC CAAATACAAT GAAGCTCAAG
481TGGACTTCCG AGAACGCAGC AAAGGGCGAA TCCAGCGGCA GCTCGAAATT ACTGGCAAAA
541AGACAACCGA TGAGGAGCTG GAGGAGATGT TGGAGAGTGG CAACCCGGCC ATCTTCACTT
601CTGGGATCAT TGACTCACAG ATTTCCAAGC AAGCCCTCAG TGAGATTGAG GGACGACACA
661AGGACATTGT GAGGCTGGAG AGCAGCATCA AGGAGCTTCA CGACATGTTT ATGGACATCG
721CCATGCTGGT GGAGAATCAG GGTGAGATGT TAGATAACAT AGAGTTGAAT GTCATGCACA
781CAGTGGACCA CGTGGAGAAG GCACGAGATG AAACGAAAAA AGCTGTGAAA TACCAGAGTC
841AGGCCCGGAA GAAATTGATA ATTATCATTG TGCTAGTAGT TGTGTTGCTG GGCATTTTAG
901CATTGATTAT TGGACTTTCC GTTGGGCTGA ATTACCCAAC TTTCTTGTAC AAAGTtggca
961ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)